Four flavonoid glycosyltransferases present in tea overexpressed in model plants Arabidopsis thaliana and Nicotiana tabacum for functional identification

Jiang, Xiaolan; Dai, Xinlong; Zhuang, Juhua; Fu, Zhouping; Zhao, Xianqian; Liu, Yajun; Gao, Liping; Xia, Tao

doi:10.1016/j.jchromb.2018.09.033

Cited by 20 publications

(15 citation statements)

References 32 publications

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“…Total flavonol levels responded to supplementary UV-B exposure in ‘AJBC’ and ‘SCZ’ leaves but not in ‘HK’ and ‘ZJ’ leaves (Figure B). Six flavonol glycosides were identified by LC–MS analysis, , with M-3- O -glu, Q-3- O -gal, and K-3- O -gal-rut as more UV-B responsive than M-3- O -gal, Q-3- O -glu, and K-3- O -gal (Figure C). In comparison to flavonols, the major catechins (C, EC, GC, EGC, CG, EGCG, GCG, and ECG) were not very responsive to supplementary UV-B exposure (panels D and E of Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, hy5 mutants contained relatively lower flavonol levels and had no significant UV-B response. Five flavonol glycosides were identified from 21-day-old seedlings by LC−MS, 4,45 and UV-B induction was detected in the accumulation of Q-3-O-rut and K-3-O-rut (Figure 5D and Figure S3 and Table S5 of the Supporting Information). Supplementary UV-B Stimulates CsHY5 and CsMYB12 Expression and Flavonol Accumulation in Tea Plants.…”

Section: Journal Ofmentioning

confidence: 99%

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Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis

Lin

Liu

Zhu

et al. 2021

J. Agric. Food Chem.

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Tea leaves contain an extraordinarily high level of flavonoids that contribute to tea health benefits and flavor characteristics, but the regulatory mechanism of ambient ultraviolet B (UV-B) on tea flavonoid enrichment remains unclear. Here, we report that ambient UV-B modulates tea quality by inducing a metabolic flux in flavonoid biosynthesis. UV-B absence decreased bitter- and astringent-tasting flavonol glycosides (kaempferol-7-O-glucoside, myricetin-3-O-glucoside, and quercetin-7-O-glucoside) but increased non-galloylated catechins. Conversely, supplementary UV-B increased flavonols and decreased catechins in tea leaves. These responses were achieved via CsHY5, which mediates the UV-B-induced MYB12 activation and binds to the promoters of flavonoid biosynthetic genes (CsFLS, CsLARa, and CsDFRa), leading to flavonoid changes. Transcriptomic data indicated that UV-B-induced tea flavonoid regulation is responsive to multiple biotic and abiotic environmental stresses. These findings improve our understanding of light-regulated tea astringency and bitterness underlying shading effects and seasonal light changes and provide novel insights into tea cultivation management and processing.

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Section: Resultsmentioning

confidence: 99%

Section: Journal Ofmentioning

confidence: 99%

Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis

Lin

Liu

Zhu

et al. 2021

J. Agric. Food Chem.

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“…As one of the world’s three largest nonalcoholic beverages, tea is known as a healthy beverage in the 21st century [ 20 ]. Today, nearly half of the world’s people drink tea.…”

Section: Introductionmentioning

confidence: 99%

Implications of endophytic microbiota in Camellia sinensis : a review on current understanding and future insights

et al. 2020

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Endophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host's growth but also holobiont resilience to abiotic and biotic stresses, providing a natural reservoir and a promising solution for sustainable agricultural development challenged by global climate change. Moreover, possessing the talent to produce a wide array of high-value natural products, plant endophytic microbiota also serve as an alternative way for novel drug discovery. In this review, tea, one of the world's three largest nonalcoholic beverages and a worldwide economic woody crop, was highlighted in the context of endophytic microbiota. We explore the recent studies regarding isolation approaches, distribution characteristics and diversity, and also biological functions of endophytic microbiota in Camellia sinensis (L.) O. Kuntze. Profoundly, the future insight into interaction mechanism between endophytic microbiota and tea plants will shed light on in-depth exploration of tea microbial resources.

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“…The metabolites in leaves of Camellia plants, Chinese bayberry plants, and Arabidopsis plants and strawberry fruits were extracted using previously described methods (Jiang et al., 2018). Freeze‐dried sample (0.05 g) was extracted four times with 4 ml of 80% methanol.…”

Section: Methodsmentioning

confidence: 99%

New insights into the function of plant tannase with promiscuous acyltransferase activity

et al. 2022

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SUMMARY Plant tannases (TAs) or tannin acyl hydrolases, a class of recently reported carboxylesterases in tannin‐rich plants, are involved in the degalloylation of two important groups of secondary metabolites: flavan‐3‐ol gallates and hydrolyzable tannins. In this paper, we have made new progress in studying the function of tea (Camellia sinensis) (Cs) TA—it is a hydrolase with promiscuous acyltransferase activity in vitro and in vivo and promotes the synthesis of simple galloyl glucoses and flavan‐3‐ol gallates in plants. We studied the functions of CsTA through enzyme analysis, protein mass spectrometry, and metabolic analysis of genetically modified plants. Firstly, CsTA was found to be not only a hydrolase but also an acyltransferase. In the two‐step catalytic reaction where CsTA hydrolyzes the galloylated compounds epigallocatechin‐3‐gallate or 1,2,3,4,6‐penta‐O‐galloyl‐β‐d‐glucose into their degalloylated forms, a long‐lived covalently bound Ser159‐linked galloyl–enzyme intermediate is also formed. Under nucleophilic attack, the galloyl group on the intermediate is transferred to the nucleophilic acyl acceptor (such as water, methanol, flavan‐3‐ols, and simple galloyl glucoses). Then, metabolic analysis suggested that transient overexpression of TAs in young strawberry (Fragaria × ananassa) fruits, young leaves of tea plants, and young leaves of Chinese bayberry (Myrica rubra) actually increased the total contents of simple galloyl glucoses and flavan‐3‐ol gallates. Overall, these findings provide new insights into the promiscuous acyltransferase activity of plant TA.

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Four flavonoid glycosyltransferases present in tea overexpressed in model plants Arabidopsis thaliana and Nicotiana tabacum for functional identification

Cited by 20 publications

References 32 publications

Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis

Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis

Implications of endophytic microbiota in Camellia sinensis : a review on current understanding and future insights

New insights into the function of plant tannase with promiscuous acyltransferase activity

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