Four Events of Host Switching in Aspidoderidae (Nematoda) Involve Convergent Lineages of Mammals

Jiménez, Francisco; Gardner, Scott Lyell; Navone, Graciela Teresa; Ortı́, Guillermo

doi:10.1645/ge-3045.1

Cited by 17 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first genetic sequences of A. raillieti from D. albiventris and the first sequences of the 18S rRNA and 28S rRNA gene for aspidoderids are presented herein. Previous sequences of A. raillieti were performed for mitochondrial cox-1 and 16S genes and ribosomal ITS1, 5.8S and ITS2 region from different hosts and localities, such as Didelphis pernigra from Bolivia, D. marsupialis from Guatemala and D. virginia from United States (Jiménez-Ruiz et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study performed with fecal samples from dasypodid hosts such as D. novemcinctus, Dasypus septemcinctus (Linnaeus 1758), Euphractus sexcinctus (Linnaeus 1758) and Tolypeutes tricinctus (Linnaeus 1758) collected in the park reported the presence of morphotypes of nematode eggs identified as Aspidoderidae Skrjabin and Schikhobalova, 1947and Trichostrongyloidea Cram, 1927(Brandão et al 2009. The family Aspidoderidae currently includes 17 species divided into four genera, and species of the genus Aspidodera have been reported in a wide range of hosts (Santos et al 1990;Jiménez-Ruiz et al 2012). These parasites are found in the cecum and large intestine of mammals of the orders Cingulata, Didelphimorphia, andRodentia (Jimenez-Ruiz et al 2012, Chagas-Moutinho et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the species of the genus Aspidodera include A. scoleciformis (Diesing, 1851), A. subulata (Molin, 1860), A. fasciata (Schneider, 1866), A. binansata Railliet and Henry, 1913, A. raillieti Travassos, 1913, A. ansirupta Proença, 1937, A. vazi Proença, 1937, A. lacombae Vicente, 1964, A. esperanzae Fujita et al 1995, A. sogandaresi Jiménez-Ruiz, Gardner & Varela-Stokes, 2006, A. kinsellai Jiménez-Ruiz, Carreno & Gardner, 2013and A. lanfredi Chagas-Moutinho et al 2014. Among these species, the presence of A. raillieti has been reported in several localities in a wide variety of hosts (Jiménez-Ruiz et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Previous molecular studies of A. raillieti were performed by Jiménez-Ruiz et al (2012) and Jiménez-Ruiz et al (2013), who obtained sequences of spanning mitochondrial cytochrome c-oxidase subunit 1 (cox-1), 16S rRNA and ITS1, 5.8S and ITS2. On the other hand, A. binansata was studied for 16S rRNA and ITS1, 5.8S and ITS2 genes (Jiménez-Ruiz et al 2012). However, there are no sequences for A. vazi available in the GenBank database so far.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

MORPHOLOGICAL AND GENETIC CHARACTERIZATION OF NEMATODES OF THE OPOSSUM Didelphis albiventris AND THE ARMADILLO Dasypus novemcinctus FROM SERRA DA CAPIVARA NATIONAL PARK

et al. 2019

View full text Add to dashboard Cite

Serra da Capivara National Park (SCNP) in Northeast Brazil has the largest concentration of prehistoric sites in the Americas where studies of coprolites of ancestral parasitic fauna reported the presence of morphotypes of nematode eggs identified as Aspidoderidae and Trichostrongyloidea from dasypodid hosts. Among the current mammals inhabiting the park, the opossum Didelphis albiventris and the armadillo Dasypus novemcinctus were found roadkill in the nearby area. The aim of this work was to perform an integrative taxonomic analysis of adult nematode parasites of D. albiventris (N = 3) and D. novemcinctus (N = 2) found roadkill in the SCNP using morphological, ultrastructural and genetic approaches. The nematodes studied included Aspidodera raillieti and Aspidodera subulata (Aspidoderidae) collected from D. albiventris, and Aspidodera binansata, Aspidodera vazi (Aspidoderidae) and Hadrostrongylus speciosum (Trichostrongylidae) from D. novemcinctus. A new geographic locality of four species of Aspidoderidae and one Trichostrongylidae species are reported with new ultrastructural data using scanning electron microscopy of A. binansata and A. vazi. New genetic data of aspidoderids include sequences of partial 18S rRNA (SSU) and partial 28S rRNA (LSU) genes of A. raillieti, the first sequence of 16S rRNA of A. vazi and additional sequences of mitochondrial DNA cytochrome c-oxidase subunit 1, internal transcribed spacer 1, 2 and 5.8S rRNA gene of A. raillieti. These are the first partial sequences of 18S rRNA (SSU) and 28S rRNA (LSU) genes of species of the family Aspidoderidae, linked to a vouchered specimen.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MORPHOLOGICAL AND GENETIC CHARACTERIZATION OF NEMATODES OF THE OPOSSUM Didelphis albiventris AND THE ARMADILLO Dasypus novemcinctus FROM SERRA DA CAPIVARA NATIONAL PARK

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was extracted, isolated, and purified from three vouchered nematodes following standard protocols [ 12 , 16 ]. These aliquots were used as a template to amplify a fragment of the mitochondrial gene coding for the large ribosomal subunit RNA ( rrnL ); the primers and thermal profile used to complete the reactions, as well as the postamplification processing of these fragments, are identical to those described elsewhere [ 11 , 16 ]. Published sequences of available herpetostrongyles, heligmosomoids, heligmonellids, and viannaids were downloaded from GenBank, aligned using Clustal Omega ( http://www.ebi.ac.uk/Tools/msa/clustalo/ ) and analyzed for phylogenetic signal using Parsimony and Maximum Likelihood as optimality criteria in PAUP* v4.b10 [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Guerrerostrongylus marginalisn. sp. (Trichostrongyloidea: Heligmonellidae) from the Guianan arboreal mouse (Oecomys auyantepui) from French Guiana

2016

Self Cite

View full text Add to dashboard Cite

Based on the number and arrangement of cuticular ridges and configuration of the dorsal ray, nematode specimens collected from the small intestine of eight Guianan arboreal mice, Oecomys auyantepui (Rodentia: Sigmodontinae), in French Guiana are herein described and characterized. Guerrerostrongylus marginalis n. sp. (Heligmosomoidea: Heligmonellidae) shows a synlophe consisting of more than 40 ridges and a unique bursal arrangement with ray 8 (externo-dorsal) extending to the edge of the bursal margin, and appearing more prominent than the dorsal ray. This bursal arrangement is common in members of Hassalstrongylus Durette-Desset, 1971, but uncommon in the other four species in Guerrerostrongylus Sutton & Durette-Desset, 1991. The placement of the new species in Guerrerostrongylus is based on the number and nature of cuticular ridges and the ray arrangement and symmetry of the caudal bursa. Diagnostic characteristics of Guerrerostrongylus marginalis n. sp. include the length of ray 8 relative to bursal margin, the relative size of the spicules and vestibule, and the number of eggs in the uterus. We propose an amendment to the generic diagnosis of Guerrerostrongylus to modify the characters of the long rays 6 (postero-lateral), rays 8 (externo-dorsal), and dorsal ray as diagnostic, since at least ray 6 appears to be short in two different species in the genus, namely G. ulysi Digiani, Notarnicola & Navone, 2012 and G. marginalis n. sp.

show abstract

Molecular identification of Heterakis spumosa obtained from brown rats (Rattus norvegicus) in Japan and its infectivity in experimental mice

et al. 2014

View full text Add to dashboard Cite

Heterakis spumosa is a nematode of invasive rodents, mainly affiliated with Rattus spp. of Asian origin. Despite the ecological importance and cosmopolitan distribution, little information is available on the genetic characteristics and infectivity to experimental animals of this roundworm. Heterakis isolates obtained from naturally infected brown rats caught in 2007 in the city of Sagamihara, east central Honshu, Japan, and maintained by laboratory passages were subjected to mitochondrial sequence analysis and experimental infection in mice. Sequencing of the cox1 gene revealed that nucleotides of H. spumosa and previously examined Heterakis isolonche isolates from gallinaceous birds in Japan differed by 11.2-12.2% that conforms to the range expected for interspecific differences. The two H. spumosa isolates differed by a single 138T/C non-synonymous substitution in the 393-bp mt sequence. In a dendrogram, the H. spumosa samples formed a subcluster with members of the nematode superfamily Heterakoidea, H. isolonche and Ascaridia galli. In an experimental infection study, ICR, AKR, B10.BR and C57BL/6 mice strains were inoculated with 200 H. spumosa eggs/head and necropsied at 14 and 90 days post-inoculation (DPI) when the number of worms was recorded. Eggs were initially detected in faeces from 32-35 DPI in ICR, AKR and B10.BR mice and the highest mean number of eggs per gram of faeces (EPG) was 4,800 at 38 DPI, 2,200 at 58 DPI and 800 at 44 and 72 DPI in ICR, AKR and B10.BR mice, respectively. No eggs were observed in faeces of the C57BL/6 mouse strain during the experiment. A similar number of juvenile worms were isolated from all mouse strains at 14 DPI, whereas no adult worms were detected in C57BL/6 mice at 90 DPI.

show abstract

Four Events of Host Switching in Aspidoderidae (Nematoda) Involve Convergent Lineages of Mammals

Cited by 17 publications

References 49 publications

MORPHOLOGICAL AND GENETIC CHARACTERIZATION OF NEMATODES OF THE OPOSSUM Didelphis albiventris AND THE ARMADILLO Dasypus novemcinctus FROM SERRA DA CAPIVARA NATIONAL PARK

MORPHOLOGICAL AND GENETIC CHARACTERIZATION OF NEMATODES OF THE OPOSSUM Didelphis albiventris AND THE ARMADILLO Dasypus novemcinctus FROM SERRA DA CAPIVARA NATIONAL PARK

Guerrerostrongylus marginalisn. sp. (Trichostrongyloidea: Heligmonellidae) from the Guianan arboreal mouse (Oecomys auyantepui) from French Guiana

Molecular identification of Heterakis spumosa obtained from brown rats (Rattus norvegicus) in Japan and its infectivity in experimental mice

Contact Info

Product

Resources

About