Four differentially expressed cDNAs in Callinectes sapidus containing the Rebers–Riddiford consensus sequence

Wynn, Anna; Shafer, Thomas H.

doi:10.1016/j.cbpc.2005.04.002

Cited by 24 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The proteins of most of the differentially expressed cuticle genes have either been isolated from uncalcified, arthrodial membranes of calcifying crustaceans, such as AMP8.1, AMP16.3 and AMP16.5 from Callinectes sapidus [22,23], or found to have an inhibitory role in calcification, for example the calcification associated peptides 1 and 2, isolated from the crayfish Procambarus clarkii [24,25]. There were only four cuticle proteins that contained the cuticle_1 domain, and although proteins with this domain have been isolated from the calcified cuticle of the crab, Cancer pagurus [26] and lobster, Homarus americanus [27], they did not include these four.…”

Section: Resultsmentioning

confidence: 99%

Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)

et al. 2010

View full text Add to dashboard Cite

BackgroundAll crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in non-calcifying organisms such as krill. To address this we constructed an Antarctic krill cDNA microarray in order to generate gene expression profiles across the moult cycle and identify possible activation pathways.ResultsA total of 26 different cuticle genes were identified that showed differential gene expression across the moult cycle. Almost all cuticle genes were up regulated during premoult and down regulated during late intermoult. There were a number of transcripts with significant sequence homology to genes potentially involved in the synthesis, breakdown and resorption of chitin. During early premoult glutamine synthetase, a gene involved in generating an amino acid used in the synthesis of glucosamine, a constituent of chitin, was up regulated more than twofold. Mannosyltransferase 1, a member of the glycosyltransferase family of enzymes that includes chitin synthase was also up regulated during early premoult. Transcripts homologous to a β-N-acetylglucosaminidase (β-NAGase) precursor were expressed at a higher level during late intermoult (prior to apolysis) than during premoult. This observation coincided with the up regulation during late intermoult, of a coatomer subunit epsilon involved in the production of vesicles that maybe used to transport the β-NAGase precursors into the exuvial cleft. Trypsin, known to activate the β-NAGase precursor, was up regulated more than fourfold during premoult. The up regulation of a predicted oligopeptide transporter during premoult may allow the transport of chitin breakdown products across the newly synthesised epi- and exocuticle layers.ConclusionWe have identified many genes differentially expressed across the moult cycle of krill that correspond with known phenotypic structural changes. This study has provided a better understanding of the processes involved in krill moulting and how they may be controlled at the gene expression level.

show abstract

Section: Resultsmentioning

confidence: 99%

Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)

et al. 2010

View full text Add to dashboard Cite

show abstract

“…For example, the Rebers-Riddiford chitin-binding domain was identified in proteins from the exoskeleton of www.chembiochem.org crustaceans. [33,34] Additionally, the Gly/Tyr-rich region of Prismalin-14 from the prismatic layer of P. fucata has a chitin-binding ability. [35,36] BMSP 120 contained the Peritrophin A chitin-binding domain that is conserved in various animal species; [37] this was first identified in Pif 97 among many matrix proteins from the nacreous layer.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterisation of a Calcium Carbonate‐Binding Protein, Blue Mussel Shell Protein (BMSP), from the Nacreous Layer

et al. 2011

View full text Add to dashboard Cite

The nacreous layer of molluscan shells consists of a highly organised, layered structure comprising calcium carbonate aragonite crystals, each surrounded by an organic matrix. In the Japanese pearl oyster Pinctada fucata, the Pif protein from the nacreous layer functions in aragonite binding, and plays a key role in nacre formation. Here, we investigated whether the blue mussel Mytilus galloprovincialis also has a protein with similar functions in the nacreous layer. By using a calcium carbonate-binding assay, we identified the novel protein blue mussel shell protein (BMSP) 100 that can bind calcium carbonate crystals of both aragonite and calcite. When the entire sequence of a cDNA encoding BMSP 100 was determined, it was found that BMSP is a preproprotein consisting of a signal peptide and two proteins, BMSP 120 and BMSP 100. BMSP 120 contains four von Willebrand factor A (VWA) domains and one chitin-binding domain, thus suggesting that it has a role in maintaining structure within the matrix. Immunohistochemical analysis revealed that BMSP 100 is present throughout the nacreous layer with dense localisation in the myostracum. Posttranslational modification analysis indicated that BMSP 100 is phosphorylated and glycosylated. These results suggest that there is a common molecular mechanism between P. fucata and M. galloprovincialis that underlies the nacreous layer formation.

show abstract

“…Biomineralization is a universal process, occurring in both prokaryotes and eukaryotes, including mammals. Biomineralization research is also a diverse and multidisciplinary field, encompassing microscopic analyses of biominerals and tissues (21,23), mineralogy (25), organic chemistry (30), paleontology (3), and cellular biochemistry (19).…”

mentioning

confidence: 99%

Catalytic Biomineralization of Fluorescent Calcite by the Thermophilic Bacterium Geobacillus thermoglucosidasius

Yoshida

Higashimura

Saeki

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Four differentially expressed cDNAs in Callinectes sapidus containing the Rebers–Riddiford consensus sequence

Cited by 24 publications

References 26 publications

Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)

Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)

Identification and Characterisation of a Calcium Carbonate‐Binding Protein, Blue Mussel Shell Protein (BMSP), from the Nacreous Layer

Catalytic Biomineralization of Fluorescent Calcite by the Thermophilic Bacterium Geobacillus thermoglucosidasius

Contact Info

Product

Resources

About