Four compartment method as an efficacious and simplified technique for autologous non‐cultured epidermal cell suspension preparation in vitiligo surgery: A randomized, active‐controlled study

Abstract

Four compartment method is a simple and effective technique for vitiligo surgery in routine clinical practice.

Vitiligo surgery: A journey from tissues via cells to the stems!

“…In this method, the laborious procedure that needs to be conducted in a sophisticated laboratory was simplified to undergo in a simple petri dish. We have found a similar repigmentation outcome with 4C method compared to the conventional lab‐based method . Benzekri et al.…”

Autologous Non-Cultured Epidermal Cellular Grafting in the Surgical Treatment of Stable Vitiligo: The Skin Hospital Protocol

“…The modified approach using an intravenous giving set with filter provided adequate filtration of a mixed cell population with high viability and proliferative potential, without having to perform repeated syringing, as has been previously described [3, 5]. Less than 1 mL of PBS solution was used to prepare the cell suspension, in order to minimise dilution and increase the viscosity to a gel-like paste.…”

Comparison of efficacy of cold trypsinization versus warm trypsinization in preparation of autologous non‐cultured epidermal cell suspension for treatment of stable vitiligo

“…However, most of the studies of vitiligo surgery have similar sample sizes and follow-up period. 5,9 Our study suggests that NCES is an effective and safe procedure for the management of stable vitiligo with cold trypsinization being superior to warm trypsinization. Editor Necrotizing fasciitis (NF) is a severe soft-tissue infection, with significant morbidity and mortality.…”