The use of X-ray microtomography (micro-CT) to evaluate the contrasts in permeability associated with bioturbation in twoand three-dimensions (2D and 3D) is investigated in this article. Core analysis, spot-permeametry measurements, and petrographic thin-sections of the six different datasets from Western Canada (Debolt Formation, Wabamun Group, and Medicine Hat Member) and offshore Norway (Ula Formation, Lysing Formation, and Nise Formation) show that the trace fossils commonly modify the permeability and porosity of the reservoir through sediment reworking and diagenesis of the sediment. In this study, micro-CT techniques are used to analyze the X-ray attenuation associated with bioturbation in siliciclastics and carbonates. Because X-ray attenuation represents the transmissivity or absorptivity of a sample to the incident X-rays, each of which is related to sediment composition and sediment densities, micro-CT images are integrated with sedimentological data and petrographic data to identify the trace fossils and matrix in 2D and 3D. Due to the difference in sediment grain size and sorting (i.e., porosity) within the burrows and matrix, analysis of the X-ray attenuations allows for their delineation in 2D cross-sectional images. When processed as 3D volumes, the micro-CT images show the complexity in burrow connectivity and burrow orientations within a sample. More importantly, the 3D volumes help show the distribution of porosity that can be linked to measured permeability values within a sample. Quantification of the heterogeneous burrow fabric on reservoir and resource quality is made from these processed micro-CT images.