Fos‐Like Immunoreactivity Induced by Seizure in Mice Is Specifically Associated With Euchromatin in Neurons

Mugnaini, Enrico; Berrebi, Albert S.; Morgan, James I.; Curran, Tom

doi:10.1111/j.1460-9568.1989.tb00773.x

Cited by 67 publications

(13 citation statements)

References 30 publications

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“…However, our results are consistent with the investigations of Cohen et al [27], who reported a complex distribution of Fos-IR in the testis of the European red fox. The localization of Fos-IR at the ultrastructural level differs somewhat from findings of previous studies [40,41]. While those investigators found Fos-IR diffusely in euchromatin and as granular aggregates of 15-20 nm in diameter, we observed Fos-IR mainly in larger, dot-like areas.…”

Section: Expression Of C-fos Mrna and Proteincontrasting

confidence: 83%

Expression of Immediate Early Genes in Tubular Cells of Rat Testis

Schultz¹,

Penttilä²,

Parvinen³

et al. 1995

Biology of Reproduction

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In this study we investigated the expression of the immediate early genes (IEGs) c-fos, c-jun, and junD on mRNA and protein levels during the spermatogenic cycle of the rat using Northern blotting, in situ hybridization, and immunocytochemistry. The expression of these IEG mRNAs and proteins exhibited stage-specific variations. The results suggest that IEGs take part in transcriptional events that are involved in regulating the proliferation and differentiation of spermatogenic cells during specific stages of the cycle of the seminiferous epithelium.

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Section: Expression Of C-fos Mrna and Proteincontrasting

confidence: 83%

Expression of Immediate Early Genes in Tubular Cells of Rat Testis

Schultz¹,

Penttilä²,

Parvinen³

et al. 1995

Biology of Reproduction

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“…Our immuno-ultrastructural observations add to a small group of studies examining c-Fos at the electron microscope level in the central nervous system: in the mouse hippocampus after Metrazole-induced seizures (Mugnaini et al, 1989), in the rat neostriatum after intrastriatal quinolinic acid (Aronin et al, 1991) and in the rat supraoptic nucleus after chronic intermittent salt loading (Lafarga et a i , 1993). Immunoelectron microscopy also showed c-Fos immunoreactivity in cortical cells of the zona fasciculata of ACTH-injected rats (Yang et al, 1990).…”

Section: Technical Considerationsmentioning

confidence: 59%

Light‐induced c‐Fos Expression in the Mouse Suprachiasmatic Nucleus: Immunoelectron Microscopy Reveals Co‐localization in Multiple Cell Types

Castel¹,

Belenky²,

Cohen³

et al. 1997

Eur J of Neuroscience

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Although light is known to regulate the level of c-fos gene expression in the suprachiasmatic nucleus (SCN), the site of an endogenous circadian clock, little is known about the identities of the photically activated cells. We used light-microscopic immunocytochemistry and immunoelectron microscopy to detect c-Fos protein in the SCN of Sabra mice exposed to brief nocturnal light pulses at zeitgeber time 15-16. Stimulation with light pulses that saturated the phase-shifting response of the circadian locomotor rhythm revealed an upper limit to the number of photo-inducible c-Fos cells at about one-fifth of the estimated total SCN cell population. This functionally defined set was morphologically and phenotypically heterogeneous. About 24% could be labelled for vasoactive intestinal polypeptide, 13% for vasopressin-neurophysin, and 7% for glial fibrillary acidic protein. The remaining 56% of c-Fos-positive cells were largely of unknown phenotype, although many were presumptive interneurons, some of which were immunoreactive for nitric oxide synthase.

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“…20 Electron microscopy has shown that neuronal excitation induces Fos immunoreactivity within the nuclei of neurons but not within glial or endothelial cells. 21 Therefore, Fos expression could be a useful marker of neuronal activation at the single cell level. In the present study, we found that pulsatile compression of the RVLM increased the number of RVLM neurons with Fos immunoreactivity in the nuclei.…”

Section: Discussionmentioning

confidence: 99%

Pressor Response to Compression of the Ventrolateral Medulla Mediated by Glutamate Receptors

et al. 1999

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Abstract-The rostral ventrolateral medulla (RVLM) is considered a major center for the regulation of sympathetic and cardiovascular activities. Several clinical studies have indicated a possible causal relationship between neurovascular contact of the left RVLM and essential hypertension, and some investigators have suggested that the left RVLM is more sensitive to pulsatile compression than the right RVLM. Previously, we reported that pulsatile compression of the RVLM elevates arterial pressure by enhancing sympathetic outflow in rats; however, we have not investigated the laterality of the responses to the compression. In addition, it remains to be elucidated whether RVLM neurons are activated by compression and, if so, how they are activated. Therefore, we performed compression experiments in rats to investigate these issues. Pulsatile compression was performed on the unilateral RVLM with a pulsating probe in anesthetized and artificially ventilated rats. Pulsatile compression of the unilateral RVLM increased arterial pressure, heart rate, and sympathetic nerve activity. The pressor response to compression was inhibited significantly after local microinjection of glutamate receptor antagonists. Pulsatile compression of the RVLM increased Fos immunoreactivitiy, a marker of neuronal activation, within the nuclei of postsynaptic RVLM neurons. All results were observed symmetrically. The data indicate that the responses to pulsatile compression of the unilateral RVLM are similar on both sides. They also suggest that pulsatile compression of the RVLM increases sympathetic and cardiovascular activities by activating postsynaptic RVLM neurons through the stimulation of the local glutamate receptors in rats. (Hypertension. 1999;33:1207-1213.)Key Words: neurons Ⅲ receptors, glutamate Ⅲ ventrolateral medulla Ⅲ sympathetic nervous system Ⅲ arterial pressure T he rostral ventrolateral medulla (RVLM) contains neurons that are the major tonic source of supraspinal sympathoexcitatory outflow 1,2 ; thus, this area is considered an important center that regulates sympathetic and cardiovascular activities.It has been reported that the posterior inferior cerebellar artery, anterior inferior cerebellar artery, or vertebral artery occasionally compresses the medulla oblongata in humans. Several clinical and necropsy studies have indicated a possible association between essential hypertension and neurovascular contact of the ventrolateral medulla at the root-entry zone of the glossopharyngeal and vagus nerves (ie, at the RVLM). [3][4][5] We reported previously that the incidence of observed neurovascular contact of the RVLM in an essential hypertension group was significantly higher than that in a secondary hypertension group or a normotensive group by use of MRI with a high-resolution matrix. 6,7 Thus, we hypothesized that neurovascular contact of the RVLM might be, at least in part, causally related to essential hypertension.It has been shown that microvascular decompression of the RVLM on the left but not the right side improve...

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Fos‐Like Immunoreactivity Induced by Seizure in Mice Is Specifically Associated With Euchromatin in Neurons

Cited by 67 publications

References 30 publications

Expression of Immediate Early Genes in Tubular Cells of Rat Testis

Expression of Immediate Early Genes in Tubular Cells of Rat Testis

Light‐induced c‐Fos Expression in the Mouse Suprachiasmatic Nucleus: Immunoelectron Microscopy Reveals Co‐localization in Multiple Cell Types

Pressor Response to Compression of the Ventrolateral Medulla Mediated by Glutamate Receptors

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