Fos and serotonin immunoreactivity in the raphe nuclei of the cat during carbachol-induced active sleep: A double-labeling study

Yamuy, Jack; Sampogna, Sharon; Rodríguez, Fernando López; Luppi, Pierre‐Hervé; Morales, F.R.; Chase, Michael H.

doi:10.1016/0306-4522(94)00633-g

Cited by 43 publications

(29 citation statements)

References 49 publications

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“…It is possible that such a localized increase might have been masked in the present, which used a single count for the entire nucleus. However, consistently with our data, few dorsal raphe serotonergic neurons expressed c-Fos during wakefulness in cats (Yamuy et al, 1995) or gerbils (Januǒnis and Fite, 2001), and none expressed c-Fos after spontaneous or forced wakefulness in rats (Lu et al, 2006). Taken together, these findings suggest that the lack of c-Fos induction in dorsal raphe serotonergic neurons after the low doses of caffeine probably does not indicate the absence of activity of these neurons.…”

Section: Caffeine-induced Neuronal Activation In Brainstem Arousal Resupporting

confidence: 89%

Differential c‐Fos immunoreactivity in arousal‐promoting cell groups following systemic administration of caffeine in rats

Deurveilher

Murphy

et al. 2006

J of Comparative Neurology

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Despite the widespread use of caffeine, the neuronal mechanisms underlying its stimulatory effects are not completely understood. By using c-Fos immunohistochemistry as a marker of neuronal activation, we recently showed that stimulant doses of caffeine activate arousal-promoting hypothalamic orexin (hypocretin) neurons. In the present study, we investigated whether other key neurons of the arousal system are also activated by caffeine, via dual immunostaining for c-Fos and transmitter markers. Rats were administered three doses of caffeine or saline vehicle during the light phase. Caffeine at 10 and 30 mg/kg, i.p., increased motor activities, including locomotion, compared with after saline or a higher dose, 75 mg/kg. The three doses of caffeine induced distinct dose-related patterns of c-Fos immunoreactivity in several arousal-promoting areas, including orexin neurons and adjacent neurons containing neither orexin nor melanin-concentrating hormone; tuberomammillary histaminergic neurons; locus coeruleus noradrenergic neurons; noncholinergic basal forebrain neurons that do not contain parvalbumin; and nondopaminergic neurons in the ventral tegmental area. At any dose used, caffeine induced little or no c-Fos expression in cholinergic neurons of the basal forebrain and mesopontine tegmentum; dopaminergic neurons of the ventral tegmental area, central gray, and substantia nigra pars compacta; and serotonergic neurons in the dorsal raphe nucleus. Saline controls exhibited only few c-Fos-positive cells in most of the cell groups examined. These results indicate that motor-stimulatory doses of caffeine induce a remarkably restricted pattern of c-Fos expression in the arousal-promoting system and suggest that this specific neuronal activation may be involved in the behavioral arousal by caffeine.

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Section: Caffeine-induced Neuronal Activation In Brainstem Arousal Resupporting

confidence: 89%

Differential c‐Fos immunoreactivity in arousal‐promoting cell groups following systemic administration of caffeine in rats

Deurveilher

Murphy

et al. 2006

J of Comparative Neurology

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“…It is well accepted that inhibition of serotonergic cells in the dorsal raphe nucleus (DRN) and noradrenergic neurons in the locus ceruleus (LC) is a necessary prerequisite for REM sleep generation. These monoaminergic cells are active during waking, decrease activity during non-REM sleep, and become inactive during REM sleep (Heym et al, 1982;Fornal et al, 1985;Sakai, 1986;Reiner and McGeer, 1987;Yamuy et al, 1995Yamuy et al, , 1998Thakkar et al, 1998;Gervasoni et al, 2000). Evidence suggests that the quiescence of these cells during REM sleep is attributable to GABA-mediated inhibition (Levine and Jacobs, 1992;Wang et al, 1992;Siegel, 1996, 1997a,b;Gervasoni et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Preoptic Area Neurons and the Homeostatic Regulation of Rapid Eye Movement Sleep

Gvilia¹,

Turner²,

McGinty³

et al. 2006

J. Neurosci.

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“…The activity of serotonergic neurons of the DRN changes according to vigilance states; i.e., their average firing rate decreases from waking to slow wave sleep to rapid-eye-movement (REM) sleep (McGinty and Harper 1976). Recent c-fos studies indicated that the MRN have a similar pattern of discharge (Maloney et al 1999;Yamuy et al 1995). It has been demonstrated that during REM sleep when 5-HT neurons are virtually silent, extracellular GABA level increased in the DRN (Nitz and Siegel 1997) and GABAergic cells were activated in both DRN and MRN (Maloney et al 1999).…”

Section: Introductionmentioning

confidence: 99%

GABAergic Control of the Ascending Input From the Median Raphe Nucleus to the Limbic System

Varga

Sı́k

et al. 2005

Journal of Neurophysiology

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Li, Shaomin, Viktor Varga, Attila Sik, and Bernat Kocsis. GABAergic control of the ascending input from the median raphe nucleus to the limbic system. J Neurophysiol 94: 2561-2574, 2005. First published June 8, 2005 doi:10.1152/jn.00379.2005. The median raphe nucleus (MRN) is the primary source of serotonergic afferents to the limbic system that are generally considered to suppress hippocampal theta oscillations. GABA receptors are expressed in the MRN by serotonergic and nonserotonergic cells, including GABAergic and glutamatergic neurons. This study investigated the mechanisms by which the fluctuating GABA tone in the MRN leads to induction or suppression of hippocampal theta rhythm. We found that MRN application of the GABA A agonist muscimol (0.05-1.0 mM) or GABA B agonist baclofen (0.2 mM) by reverse microdialysis had strong theta promoting effects. The GABA A antagonist bicuculline infused in low concentrations (0.1, 0.2 mM) eliminated theta rhythm. A short period of theta activity of higher than normal frequency preceded hippocampal desynchronization in 46% of rats. Bicuculline in larger concentrations (0.5, 1.0, 2.0 mM) resulted in a biphasic response of an initial short (Ͻ10 min) hippocampal desynchronization followed by stable theta rhythm that lasted as long as the infusion continued. The frequency and amplitude of theta waves were higher than in control recordings and the oscillations showed a conspicuous intermittent character. Hippocampal theta rhythm elicited by MRN administration of bicuculline could be completely (0.5 mM bicuculline) or partially (1.0 mM bicuculline) blocked by simultaneous infusion of the GABA B antagonist CGP35348. Our findings suggest that the GABAergic network may have two opposing functions in the MRN: relieving the theta-generators from serotonergic inhibition and regulating the activity of a theta-promoting circuitry by the fluctuating GABA tone. The two mechanisms may be involved in different functions.

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Fos and serotonin immunoreactivity in the raphe nuclei of the cat during carbachol-induced active sleep: A double-labeling study

Cited by 43 publications

References 49 publications

Differential c‐Fos immunoreactivity in arousal‐promoting cell groups following systemic administration of caffeine in rats

Differential c‐Fos immunoreactivity in arousal‐promoting cell groups following systemic administration of caffeine in rats

Preoptic Area Neurons and the Homeostatic Regulation of Rapid Eye Movement Sleep

GABAergic Control of the Ascending Input From the Median Raphe Nucleus to the Limbic System

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