Fos and Jun oncogenes transactivate chimeric or native promoters containing AP1/GCN4 binding sites in plant cells.

Hilson, Pierre; Froidmont, D. de; Lejour, C.; Hirai, Syu-ichi; Jacquemin, Jean-Marie; Yaniv, Moshe

doi:10.1105/tpc.2.7.651

Cited by 14 publications

(2 citation statements)

References 37 publications

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“…Although they are not suited for complex organism studies, transient expression assays (TEAs) in plant protoplasts have proven very useful for dissecting a broad range of molecular mechanisms, including signalling and metabolic pathways as well as transcriptional regulatory networks (Asai et al. , 2002; Hilson et al. , 1990; Hwang and Sheen, 2001; Kovtun et al.…”

Section: Introductionmentioning

confidence: 99%

Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells

et al. 2005

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SummaryAlthough sequence information and genome annotation are improving at an impressive pace, functional ontology is still non-existent or rudimentary for most genes. In this regard, transient expression assays are very valuable for identification of short functional segments in particular pathways, because they can be performed rapidly and at a scale unattainable in stably transformed tissues. Vectors were constructed and protocols developed for systematic transient assays in plant protoplasts. To enhance throughput and reproducibility, protoplast treatments were performed entirely by a liquid-handling robot in multiwell plates, including polyethylene glycol/Ca 2þ cell transfection with plasmid mixtures, washes and lysis. All transcriptional readouts were measured using a dual firefly/Renilla luciferase assay, in which the former was controlled by a reporter promoter and the latter by the 35S CaMV promoter, which served as internal normalization standard. The automated protocols were suitable for transient assays in protoplasts prepared from cell cultures of Nicotiana tabacum Bright Yellow-2 and Arabidopsis thaliana. They were implemented in a screen to discover potential regulators of genes coding for key enzymes in nicotine biosynthesis. Two novel tobacco transcription factors were found, NtORC1 and NtJAP1, that positively regulate the putrescine N-methyltransferase (PMT) promoter. In addition, combinatorial tests showed that these two factors act synergistically to induce PMT transcriptional activity. The development and use of high-throughput plant transient expression assays are discussed.

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Section: Introductionmentioning

confidence: 99%

Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells

et al. 2005

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“…The palindromic DNA motif ATGAGTCAT, the binding site of the AP1 products of oncogenes Fos and Jun, is incomplete in the 5' flanking region of the prolamin 4a gene published by Kim and Okita (1988b consensus sequence was found intact in -3 0 0 elements of the 5' upstream region ( -4 4 3 to -4 3 5 ) , as in other cereal seed storage protein genes (Hilson et al, 1990). As for its function, Hilson et al (1990) have successfully activated the transcription of the wheat glutenin gene in tobacco suspension cells by introducing A P 1 / G C N 4 .…”

Section: Discussionmentioning

confidence: 99%

The endosperm-specific expression of a rice prolamin chimaeric gene in transgenic tobacco plants

Zhou

Fan

1993

Transgenic Research

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The 5' upstream region (-680 to +40), containing the potential promoter and complete signal peptide coding sequence of the rice seed storage prolamin gene was amplified in vitro using the polymerase chain reaction from the genome of Chinese rice cultivar Zhonghua 8. The physical map and DNA sequence analysis show strong homology with the 5' flanking region of the rice prolamin gene published by Kim and Okita (1988a). No change in the signal peptide coding sequence and a long leader sequence with several small open reading frames were found. The chimaeric gene containing the 5' flanking region of the prolamin gene (-680 to -18) was transcriptionally fused with the beta-glucuronidase (GUS) reporter gene and the fusion junction was confirmed by both physical mapping and DNA sequence analysis. The resultant chimaeric gene was used to transform tobacco explants, using the Ti binary system of Agrobacterium tumefaciens LBA4404. Three transgenic tobacco plants with as many as 20 copies of the chimaeric GUS gene (confirmed by dot and Southern hybridization) were analysed further. Histochemical analysis revealed GUS activity in the endosperm tissue of tobacco seed at the developmental stage about 20 days after flowering (DAF). No GUS activity was found in leaves, stems, roots and flowers of the transgenic tobacco plants. Therefore, we conclude that the 5' upstream region from -680 to -18 was sufficient to confer the endosperm-specific expression of the rice prolamin gene.

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A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida

et al. 1995

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The mitogen activated protein (MAP) kinase pathway of eukaryotes is stimulated by many growth factors and is required for the integration of multiple cellular signals. In order to study the function of MAP kinases during plant ovule development we have synthesized a Petunia hybrida ovule-specific cDNA library and screened for MAP protein kinase-related sequences using a DNA probe obtained by PCR. A full-length cDNA clone was identified (PMEK for Petunia hybrida MAP/ERK-related protein kinase) and shown to encode a protein related to the family of MAP/ERK protein kinases. Southern blot analysis showed that PMEK is a member of a small multigene family in P. hybrida. The cDNA codes for a protein (PMEK1) of 44.4 kDa with an overall sequence identity of 44% to the products of the mammalian ERK/MAP kinase gene, and the budding yeast KSS1 and FUS3 genes. PMEK1 displays 96 and 80% identity respectively with the tobacco NTF3 and Arabidopsis ATMPK1 kinases, and only 50% to the more distantly related plant MAP kinase MsERK1 from alfalfa. The two phosphorylation sites found in the loop between subdomain VII and VIII in all the other MAP kinases are also present in PMEK1. RNA gel blot and RT-PCR analyses demonstrated that PMEK1 is expressed in vegetative organs and preferentially accumulated in female reproductive organs of P. hybrida. In situ hybridization experiments showed that in the reproductive organs PMEK1 is expressed only in the ovary and not in the stamen.

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Fos and Jun oncogenes transactivate chimeric or native promoters containing AP1/GCN4 binding sites in plant cells.

Cited by 14 publications

References 37 publications

Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells

Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells

The endosperm-specific expression of a rice prolamin chimaeric gene in transgenic tobacco plants

A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida

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