Forward Wound Closure with Regenerated Silk Fibroin and Polylysine-Modified Chitosan Composite Bioadhesives as Dressings

Abstract

Wound dressing has been used for decades to be effective for accelerating skin wound healing. However, practical applications are still limited due to their lower cell affinity, tissue adhesiveness, and biocompatibility. Natural polymers are the important biomaterials because of their excellent biodegradability, biocompatibility, and low immunogenicity. In this work, the composite bioadhesives (PLS-CS/RSF) were prepared from regenerated silk fibroin (RSF) and polylysine-modified chitosan (PLS-CS) that were cross-linked by Ca 2+ ions. The adhesion property tests showed that the PLS-CS/RSF exhibited excellent bonding potentials for various substrates, and the adhesive strength was up to 70 kPa for isolated porcine skin by the extension test. The as-prepared PLS-CS/RSF was nontoxic, displayed obvious antibacterial effects against Staphylococcus aureus and Escherichia coli in vitro, and their bacteriostasis rates were 100% after 120 min treatment. In addition, the PLS-CS/RSF exhibited favorable cytocompatibility by cell counting kit-8 assay. The animal model of wound closure results showed that PLS-CS/ RSF can promote wound closure and the integrity of wound healing, inhibiting the secretion of inflammatory factor and tumor necrosis factor and stimulating vascular factor and α-smooth muscle actin to the release of vascular growth factor and promote angiogenesis during the process of wound healing by immunohistochemical assay.

Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue

“…Importantly, PL functionalised CS polymer may enhance the physical and chemical properties of CS with improved anti‐bacterial activity. 30 , 31 …”

Bioactive Silk Fibroin-Based Hybrid Biomaterials for Musculoskeletal Engineering: Recent Progress and Perspectives

“…Apart from musculoskeletal tissue engineering, SF-based hybrid biomaterials could also be the reasonable choice for various tissue regeneration including cardiac, vascular, hepatic, ocular tissue, and so on . To put the information in perspective, it should be mentioned that silk fibroin introduced above also have their roles in other fields such as wound dressing, drug delivery, and even gene therapy. − For example, Maity et al have investigated the injectable SF-based hydrogel to use as the active storage of insulin, releasing insulin continuously for diabetic patients . Moreover, Chen et al have applied dexamethasone-loaded injectable SF-based hydrogel to counter the cisplatin-induced ototoxicity in chemotherapy .…”

Hydrogel adhesives for generalized wound treatment: Design and applications

“…The strength of electrostatic bonding depends on the charge density, and the charge density can be adjusted by the ion concentration around the hydrogel adhesive, so the electrostatic adhesion mechanism has the potential to develop pH‐responsive hydrogel adhesives. [ 78 ] The hydrophobic interaction enhances its adhesion by fixing the hydrogel adhesive on the tissue. For example, researchers add tissue‐adhesive hydrophobized microparticles to the hydrogel adhesive to enhance its wet adhesion properties.…”