2018
DOI: 10.3389/fmicb.2018.02269
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Forward Genetics by Genome Sequencing Uncovers the Central Role of the Aspergillus niger goxB Locus in Hydrogen Peroxide Induced Glucose Oxidase Expression

Abstract: Aspergillus niger is an industrially important source for gluconic acid and glucose oxidase (GOx), a secreted commercially important flavoprotein which catalyses the oxidation of β-D-glucose by molecular oxygen to D-glucolactone and hydrogen peroxide. Expression of goxC, the GOx encoding gene and the concomitant two step conversion of glucose to gluconic acid requires oxygen and the presence of significant amounts of glucose in the medium and is optimally induced at pH 5.5. The molecular mechanisms underlying … Show more

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Cited by 14 publications
(24 citation statements)
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“…Within this 9kb region, three open reading frames (ORFs) (NRRL3_03857, NRRL3_03858, and NRRL3_03859) are located. As noted in a previous report (Laothanachareon et al, 2018), NRRL3_03857…”
Section: Genome Sequencing Of N400 N401 and N402supporting
confidence: 63%
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“…Within this 9kb region, three open reading frames (ORFs) (NRRL3_03857, NRRL3_03858, and NRRL3_03859) are located. As noted in a previous report (Laothanachareon et al, 2018), NRRL3_03857…”
Section: Genome Sequencing Of N400 N401 and N402supporting
confidence: 63%
“…Strains N401 and N402 are derivatives of wild-type A. niger strain N400 and were obtained by two consecutive rounds of UV mutagenesis [Laothanachareon et al, 2018; F. Debets (Wageningen University), personal communication]. Stalk lengths of N400, N401, and N402 were determined as described in the "Materials and Methods" section.…”
Section: Analyses Of Conidiophore Stalk Length and Radial Growth Of N400 N401 And N402mentioning
confidence: 99%
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“…Genomic comparison revealed approximately 98% similarity between the six A. flavus species and 81% similarity between A. flavus and A. parasiticus species (Faustinelli et al , 2016). Fourteen strains of A. niger have been registered, the majority of which have been related to citrate production or sugar metabolism (Aguilar‐Pontes et al , 2018; Laothanachareon et al , 2018). A. carbonarius is another ochratoxin A‐producing member of this genus, for which one strain has been sequenced (NCBI genome ID 947).…”
Section: Wgs and Genetic Marker Identification Of Related Microbes Onmentioning
confidence: 99%
“…Second, identify the causative mutation by whole exome sequencing or whole genome sequencing [21][22][23] . This method is relatively quick and avoids the need for positional cloning.…”
Section: Forward Geneticsmentioning
confidence: 99%