Fortschritte in der optischen Einzelmoleküldetektion: Auf dem Weg zu höchstempfindlichen Bioaffinitätsassays

Mickert, Matthias J.; Pastucha, Matěj; Mikušová, Zuzana; Skládal, Petr; Gorris, Hans H.

doi:10.1002/ange.201913924

“…Meanwhile, trace amounts of cancer biomarkers can indicate the very beginning of a malignant transformation but many biomarkers are only measurable when the disease has progressed due to the sensitivity limitation [25] . Therefore, detecting biomarkers with high sensitivity is essential for earlier cancer diagnosis and prevention [26] . Compared with traditional bioassays, single‐molecule ECL bioassay will be more promising in detecting trace biomarkers that present at the early stages of cancer.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Single‐Molecule Electrochemiluminescence Bioassay

Zhu

¹

,

Dong

²

,

Ruan

³

et al. 2023

Angewandte Chemie

0

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A single-molecule electrochemiluminescence bioassay is developed here which allows imaging and direct quantification of single biomolecules. Imaging single biomolecules is realized by localizing the electrochemiluminescence events of the labeled molecules. Such an imaging system allows mapping the spatial distribution of biomolecules with electrochemiluminescence and contains quantitative single-molecule insights. We further quantify biomolecules by spatiotemporally merging the repeated reactions at one molecule site and then counting the clustered molecules. The proposed single-molecule electrochemiluminescence bioassay is used to detect carcinoembryonic antigen, showing a limit of detection of 67 attomole concentration which is 10 000 times better than conventional electrochemiluminescence bioassays. This spatial resolution and sensitivity enable single-molecule electrochemiluminescence bioassay a new toolbox for both specific bioimaging and ultrasensitive quantitative analysis.

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A Universal Boronate‐Affinity Crosslinking‐Amplified Dynamic Light Scattering Immunoassay for Point‐of‐Care Glycoprotein Detection

Chen

¹

,

Hao

²

,

Hu

³

et al. 2021

Angewandte Chemie

1

0

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Herein, we report a universal boronate-affinity crosslinking-amplified dynamic light scattering (DLS) immunoassay for point-of-care (POC) glycoprotein detection in complex samples. This enhanced DLS immunoassay consists of two elements, i.e., antibody-coated magnetic nanoparticles (MNP@mAb) for target capture and DLS signal transduction, and phenylboronic acid-based boronate-affinity materials as crosslinking amplifiers. Upon the addition of targets, glycoproteins are first captured by MNP@mAb and amplified by target-induced crosslinking stemming from the selective binding between the boronic acid ligand and cisdiol-containing glycoprotein, thereby resulting in a remarkably increased DLS signal in the average nanoparticle size. Benefiting from the multivalent binding and fast boronateaffinity reaction between glycoproteins and crosslinkers, the proposed immunosensing strategy has achieved the ultrasensitive and rapid quantitative assay of glycoproteins at the fM level within 15 min. Overall, this work provides a promising and versatile design strategy for extending the DLS technique to detect glycoproteins even in the field or at POC.

show abstract

A Universal Boronate‐Affinity Crosslinking‐Amplified Dynamic Light Scattering Immunoassay for Point‐of‐Care Glycoprotein Detection

Chen

¹

,

Hao

²

,

Hu

³

et al. 2021

View full text Add to dashboard Cite

Herein, we report a universal boronate-affinity crosslinking-amplified dynamic light scattering (DLS) immunoassay for point-of-care (POC) glycoprotein detection in complex samples. This enhanced DLS immunoassay consists of two elements, i.e., antibody-coated magnetic nanoparticles (MNP@mAb) for target capture and DLS signal transduction, and phenylboronic acid-based boronate-affinity materials as crosslinking amplifiers. Upon the addition of targets, glycoproteins are first captured by MNP@mAb and amplified by target-induced crosslinking stemming from the selective binding between the boronic acid ligand and cisdiol-containing glycoprotein, thereby resulting in a remarkably increased DLS signal in the average nanoparticle size. Benefiting from the multivalent binding and fast boronateaffinity reaction between glycoproteins and crosslinkers, the proposed immunosensing strategy has achieved the ultrasensitive and rapid quantitative assay of glycoproteins at the fM level within 15 min. Overall, this work provides a promising and versatile design strategy for extending the DLS technique to detect glycoproteins even in the field or at POC.

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Fortschritte in der optischen Einzelmoleküldetektion: Auf dem Weg zu höchstempfindlichen Bioaffinitätsassays

Abstract: 625 00 Brno (Tschechische Republik) [ + + ]D iese Autoren haben zu gleichen Teilen zu der Arbeit beigetragen.

Cited by 6 publications

References 179 publications

Quantitative Single‐Molecule Electrochemiluminescence Bioassay

Quantitative Single‐Molecule Electrochemiluminescence Bioassay

A Universal Boronate‐Affinity Crosslinking‐Amplified Dynamic Light Scattering Immunoassay for Point‐of‐Care Glycoprotein Detection

A Universal Boronate‐Affinity Crosslinking‐Amplified Dynamic Light Scattering Immunoassay for Point‐of‐Care Glycoprotein Detection

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