Forsterite-hydroxyapatite composite scaffolds with photothermal antibacterial activity for bone repair

Abstract

Bone engineering scaffolds with antibacterial activity satisfy the repair of bacterial infected bone defects, which is an expected issue in clinical. In this work, 3D-printed polymer-derived forsterite scaffolds were proposed to be deposited with hydroxyapatite (HA) coating via a hydrothermal treatment, achieving the functions of photothermal-induced antibacterial ability and bioactivity. The results showed that polymer-derived forsterite scaffolds possessed the photothermal antibacterial ability to inhibit Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in vitro, owing to the photothermal effect of free carbon embedded in the scaffolds. The morphology of HA coating on forsterite scaffolds could be controlled through changing the hydrothermal temperature and the pH value of the reaction solution during hydrothermal treatment. Furthermore, HA coating did not influence the mechanical strength and photothermal effect of the scaffolds, but facilitated the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) on scaffolds. Hence, the HA-deposited forsterite scaffolds would be greatly promising for repairing bacterial infected bone defects.

3D printed bioactive calcium silicate ceramics as antibacterial scaffolds for hard tissue engineering

“…[58][59][60] Silicatebased 3D printed bioceramics (using forsterite, b-TCP, hardystonite, diopside) have revealed antibacterial properties against S. aureus, S. epidermidis and E. coli. [61][62][63][64][65] However, to the best of our knowledge, neither b-Ca 2 SiO 4 bioceramic fabricated by UV-LCD from a mixture of light-sensitive preceramic polymer resins nor its antibacterial activities have been reported to date. Building on the above concept, this study presents an innovative approach to the fabrication of porous b-Ca 2 SiO 4 scaffolds by UV-LCD, using two different preceramic resins.…”

Antimicrobial and Biodegradable 3D Printed Scaffolds for Orthopedic Infections

“…The antibacterial efficiencies of the scaffolds against S. aureus and E. coli were enhanced by 96.1% and 95.6%, respectively. 85 A customized photothermal MXene-based hydrogel scaffold (GelMA/-TCP/sodium alginate (Sr 2+ )/MXene (Ti 3 C 2 ) (GTAM) hydrogel) was developed by using 3D printing. Invitro analysis showed that the GTAM scaffolds were able to inhibit the growth of Gram-positive and -negative bacteria via NIR light.…”

“…The polymer-derived forsterite scaffolds were found to possess the photothermal in- vitro antibacterial capability under 808 nm laser irradiation to inhibit S. aureus and E. coli growth. The antibacterial efficiencies of the scaffolds against S. aureus and E. coli were enhanced by 96.1% and 95.6%, respectively …”

Bioceramic-based scaffolds with antibacterial function for bone tissue engineering: A review

“… Antibacterial mechanisms of scaffolds Bacteria used for antibacterial assays Ref. Surface charge Chitosan/zoledronic acid/nano hydroxyapatite scaffold; chitosan/zein/silica scaffold Positively charged surface disrupting the negatively charged membrane of bacteria; Covering bacterial cell wall to block transport; Penetrating bacterial cell wall to prevent DNA replication E. coli , S. aureus [ [100] , [101] , [102] ] Pressure (surface charge) Potassium-sodium niobate scaffold; (Ba,Ca) (Ti,Zr)O 3 scaffold Piezoelectric effects inducing surface charge; Surface charge generating micro-electric field and ROS around the material to kill bacteria E. coli , S. aureus [ 38 , 94 ] Photothermal effect Free carbon-containing forsterite scaffold; Forsterite-hydroxyapatite scaffold Photothermic effect generating ROS and increasing temperature to kill bacteria S. aureus , E. coli , MRSA [ 22 , 99 , 103 ] Magnetothermal effect Mg 2 SiO 4 –CoFe 2 O 4 scaffold Magnetothermal effect generating thermal energy and increasing temperature to kill bacteria S. aureus , E. coli [ 95 , 104 ] Sonodynamic effect Palacos (bone cement) scaffold Attaching to certain cellular components and inducing damage under ultrasound irradiation; Generating ROS inducing oxidative damage to the cell wall MRSA, S. aureus , E. Coli, P. aeruginosa [ [105] , [106] , [107] ] Photocatalysis GDY-modified TiO 2 nanofiber s...…”