Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing

Pillai, Sreenadh Sasidharan; Yukawa, Hiroshi; Onoshima, Daisuke; Biju, Vasudevanpillai; Baba, Yoshinobu

doi:10.2116/analsci.33.137

Cited by 15 publications

(11 citation statements)

References 64 publications

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“…The analysis of correlation of MMP-2 and TIMP-3 expression with clinicopathological data can effectively explain the pathogenesis of related diseases. Previous findings have shown that MMP-2 can affect tumor angiogenesis, suggesting that MMP-2 is associated with the occurrence and development of a variety of tumors ( 18 , 19 ). Liu et al ( 20 ) found that MMP-2 is highly expressed in a variety of solid tumors, indicating that MMP-2 is closely related to the occurrence and metastasis of solid tumors.…”

Section: Discussionmentioning

confidence: 99%

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

2018

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The expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in giant-cell tumor of bone (GCT), and the correlation of their expression with the clinicopathologic features and prognosis were investigated. A total of 70 GCT patients treated in our hospital from September, 2013 to September, 2015, were selected, and the tumor and para-carcinoma tissues were obtained by surgery. The expression levels of MMP-2 and TIMP-3 in GCT and para-carcinoma tissues were detected via semi-quantitative polymerase chain reaction (PCR) and western blot analysis, as well as immunohistochemical staining. Moreover, the clinicopathological data of the GCT patients were collected to study the correlation of MMP-2 and TIMP-3 with clinicopathological features and prognosis of GCT. The results of semi-quantitative PCR and western blot analysis revealed that the expression level of MMP-2 in tissues of the 70 GCT patients was significantly higher than that in para-carcinoma tissues, and the difference was statistically significant (P<0.01), while the expression level of TIMP-3 was obviously lower than that in para-carcinoma tissues (P<0.01). The results of immunohistochemical staining revealed that the positive expression rate of MMP-2 was 57.6% in GCT tissues and 18.9% in para-carcinoma tissues, while that of TIMP-3 was 63.2% in GCT tissues and 13.8% in para-carcinoma tissues, and the differences were statistically significant (P<0.01). The expression levels of MMP-2 and TIMP-3 were correlated with the diameter of tumor, clinical staging, lymph node metastasis and relapse of GCT (P<0.01), but were not correlated with the age and sex of GCT patients (P>0.05). There was a negative correlation between MMP-2 and TIMP-3 expression levels (r=−0.258, P<0.05). The expression levels of MMP-2 and TIMP-3 are closely related to the clinicopathological features and prognosis of patients, which can be used as one of the clinical examination indexes of GCT and also provide new insights for the clinical treatment of GCT.

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Section: Discussionmentioning

confidence: 99%

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

2018

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“…The sensing of several organic and biological compounds using photoluminescent QDs has also been reported. 74,75,77,81,[84][85][86] In the case of a temperature sensor using photoluminescent QDs, 74,75,87 a typical QD-based physical sensor, the sensing principle does not involve the surface sensitivity of QDs, but changes in the Eg of QDs with any temperature variation.…”

Section: Optical Ozone Gas Sensing In Ambient Air By Reversible Ozonementioning

confidence: 99%

Development of Technologies for Sensing Ozone in Ambient Air

2018

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Ozone (O) gas is widely used as a strong oxidizing agent for many purposes, such as the decomposition/removal of organic contaminants and photoresist, and the deodorization/disinfection of air and water. However, ozone is highly toxic to the human body when the air concentration exceeds about 1 ppm. Therefore, there is increasing demand for simple, sensitive, reliable, and cost-effective techniques for sensing ozone gas. This article describes the features, advantages, and disadvantages of the available, practical techniques for sensing ozone gas in ambient air. The advantages of optical gas sensors as next-generation sensors is specifically introduced. The features of photoluminescent, semiconductor nanoparticles (quantum dots, QDs) as bright phosphors with the potential for various applications is further explored. Lastly, recent research results demonstrating the ozone sensitivity of photoluminescent CdSe-based core-shell quantum dots are presented. These results strongly suggest that optical ozone sensing using photoluminescent quantum dots is a promising technique.

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“…So far, FRET fluorescent probes have been widely reported in recent years (16)(17)(18)(19)(20). FRET fluorescent probes not only have mature applications in the detection of metal ions (21), ROS (22) and RNS (23), but also can be used to sense biological macromolecules (e.g., proteins (24)(25)(26)(27), nucleic acids (28,29)), and even be used for the visualization of changes in the cell microenvironment and the monitoring of pathophysiological processes (30)(31)(32)(33)(34). There is no doubt that FRET probes are of great significance to biological research.…”

Section: Introductionmentioning

confidence: 99%

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†

Zhu

Ouyang

et al. 2022

Photochem & Photobiology

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Fluorescent probes based on fluorescence resonance energy transfer (FRET) are highly promising for diverse bioapplications. The key to constructing FRET probes is to confine the donor and acceptor within a sufficiently close distance. However, the commonly used covalent linkage often requires elaborate design and complex organic synthesis, and sometimes causes changes in the fluorescence properties of the donor and acceptor. Inspired by the binding between small molecules and protein in nature, herein, we propose a proteinmediated strategy to fabricate FRET probe. In such proteinmediated FRET (P-FRET) probe, protein acts as a carrier to simultaneously confine donor and acceptor in its cavity. As a proof of concept, we use bovine serum albumin (BSA) as a model protein, coumarin derivative as a donor and hydroxyl radical (ÁOH)-responsive dye fluorescein as an acceptor. Through a series of investigations, including binding parameters, fluorescence properties and detection performance, we prove that the construction of P-FRET probe is simple and feasible and the detection is sensitive. Our P-FRET strategy will provide new insights for the design of FRET probes.

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Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing

Cited by 15 publications

References 64 publications

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

Development of Technologies for Sensing Ozone in Ambient Air

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†

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Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing

Cited by 15 publications

References 64 publications

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

Expression of MMP-2 and TIMP‑3 with incidence and prognosis of giant-cell tumor of the bone

Development of Technologies for Sensing Ozone in Ambient Air

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection†

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Product

Resources

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Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†