1981
DOI: 10.1073/pnas.78.4.2278
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Formycin 5'-triphosphate, a fluorescent analog of ATP, as a substrate for adenylate cyclase.

Abstract: Formycin 5'-triphosphate (FoTP), a fluorescent analog ofATP, is shown to be a substrate for the membrane-bound adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.11 from rat osteosarcoma cells. The formation of the adenylate cyclase reaction product, 3',5'-cyclic formycin monophosphate (cFoMP), was followed by the conventional radioimmunoassay (RIA) procedure used to detect cAMP and by an assay procedure in which the reaction product was separated from the substrate by reverse-phase high… Show more

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Cited by 14 publications
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