Formulation optimization of itraconazole loaded PEGylated liposomes for parenteral administration by using design of experiments

Ćurić, Anamarija; Reul, Regina; Möschwitzer, Jan P.; Fricker, Gert

doi:10.1016/j.ijpharm.2013.03.029

Cited by 33 publications

(8 citation statements)

References 19 publications

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“…The traditional approach which involves changing one variable at a time, while keeping the others constant, requires a lot of time, energy and resources. As opposed to this classical technique, experimental design allows to simultaneously modulate several input variables 46. DOE is a powerful and useful tool for evaluating the relation between a set of input variables and the investigated experimental responses.…”

Section: Resultsmentioning

confidence: 99%

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Tefas

Sylvester

Tomuță

et al. 2017

DDDT

107

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The aim of this work was to use the quality-by-design (QbD) approach in the development of long-circulating liposomes co-loaded with curcumin (CUR) and doxorubicin (DOX) and to evaluate the cytotoxic potential of these liposomes in vitro using C26 murine colon carcinoma cell line. Based on a risk assessment, six parameters, namely the phospholipid, CUR and DOX concentrations, the phospholipid:cholesterol molar ratio, the temperature during the evaporation and hydration steps and the pH of the phosphate buffer, were identified as potential risk factors for the quality of the final product. The influence of these variables on the critical quality attributes of the co-loaded liposomal CUR and DOX was investigated: particle size, zeta potential, drug loading and entrapment efficiency. For this, a 26−2 factorial design was employed to establish a proper regression model and to generate the contour plots for the responses. The obtained data served to establish the design space for which different combinations of variables yielded liposomes with characteristics within predefined specifications. The validation of the model was carried out by preparing two liposomal formulations corresponding to the robust set point from within the design space and one outside the design space and calculating the percentage bias between the predicted and actual experimental results. The in vitro antiproliferative test showed that at higher CUR concentrations, the liposomes co-encapsulating CUR and DOX had a greater cytotoxic effect than DOX-loaded liposomes. Overall, this study showed that QbD is a useful instrument for controlling and optimizing the manufacturing process of liposomes co-loaded with CUR and DOX and that this nanoparticulate system possesses a great potential for use in colon cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Tefas

Sylvester

Tomuță

et al. 2017

DDDT

107

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“…Therefore, DoE provides relevant information with a minimal number of performed experiments (20). Based on the results of these experiments the optimal run conditions can be statistically determined and the outcome of unperformed experiments predicted with high accuracy (21). Pharmaceutical applications of DoE include the development and optimization of single-component (22,23) and multi-component formulations (24,25), as well as the development and validation of analytical methods (26,27).…”

mentioning

confidence: 99%

HPLC method development for fampridine using Analytical Quality by Design approach

et al. 2020

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Offering a systematic and multivariate analysis of the analytical procedure, development and validation of HPLC methods using Quality by Design approach are in the limelight of current research trends. A new, experimental design-aided HPLC method for fampridine was developed and preliminarily validated according to current in-force international guidelines for linearity, accuracy, robustness and precision.The method offers a high throughput sample analysis, with an elution time of 2.9 minutes, and signal detection without excipient interference performed at 262 nm. The method proved to be linear between 1–15 µg mL−1 (R2= 0.9996). The mean recovery was found to be 98.7 ± 1.9 % in the tested range of 2.5–7.5 µg mL−1. Low RSD values (< 1 %) were obtained for both model, intra- and inter-day precision. The limit of detection and limit of quantification were 0.24 and 0.78 µg mL−1, resp. The method proved to be applicable for active substance assay in a pharmaceutical dosage form.

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“…240,241 Various processes can be used to prepare liposomes, such as hydration of a thin lipid film [242][243][244] followed by agitation, [245][246][247][248] sonication, [249][250][251][252][253][254] extrusion, 251,[255][256][257][258] high-pressure homogenization, [259][260][261][262] or reverse-phase evaporation. [263][264][265][266] Liposomes may contain a single lipid bilayer or multiple bilayers around the inner aqueous compartment and are therefore classified as unilamellar and multilamellar, respectively.…”

Section: Liposomesmentioning

confidence: 99%

Nanotechnology-based drug delivery systems for the treatment of Alzheimer’s disease

Fonseca-Santos¹,

Chorilli²,

Gremião³

2015

IJN

247

160

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Alzheimer’s disease is a neurological disorder that results in cognitive and behavioral impairment. Conventional treatment strategies, such as acetylcholinesterase inhibitor drugs, often fail due to their poor solubility, lower bioavailability, and ineffective ability to cross the blood–brain barrier. Nanotechnological treatment methods, which involve the design, characterization, production, and application of nanoscale drug delivery systems, have been employed to optimize therapeutics. These nanotechnologies include polymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid carriers, microemulsion, nanoemulsion, and liquid crystals. Each of these are promising tools for the delivery of therapeutic devices to the brain via various routes of administration, particularly the intranasal route. The objective of this study is to present a systematic review of nanotechnology-based drug delivery systems for the treatment of Alzheimer’s disease.

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Formulation optimization of itraconazole loaded PEGylated liposomes for parenteral administration by using design of experiments

Cited by 33 publications

References 19 publications

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

HPLC method development for fampridine using Analytical Quality by Design approach

Nanotechnology-based drug delivery systems for the treatment of Alzheimer’s disease

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Formulation optimization of itraconazole loaded PEGylated liposomes for parenteral administration by using design of experiments

Cited by 33 publications

References 19 publications

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

HPLC method development for fampridine using Analytical Quality by Design approach

Nanotechnology-based drug delivery systems for the treatment of Alzheimer&rsquo;s disease

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Product

Resources

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Nanotechnology-based drug delivery systems for the treatment of Alzheimer’s disease