Formulation of Super Saturable-Self Micro Emulsifying Loaded Centella asiatica L. Extract and FTIR-based Fingerprinting Combinated Chemometrics Analysis

Abstract

In this study, self-emulsifying method was applied to formulate the Centella asiatica L. urban extract (CeCa). The presence of groups in the extract was established on the basis of FTIR-ATR spectra using a chemometric approach. Further, the super saturable-CeCa-self micro emulsifying (SS-CeCa-SME) extracts were formulated using an oleic acid carrier oil, Tween 80 as surfactant and propylene glycol as co-surfactant with nine variations of component concentration. The FTIR-ATR spectra obtained were evaluated using a chemometric approach. The chemometric analysis method uses principal component analysis (PCA) and cluster analysis (CA). The score plot provides precise classification results by forming three large groups. The results were obtained to become a reference in the optimization process and further development of pharmaceutical preparations.