Formulation of nanoliposome-encapsulated bevacizumab (Avastin): Statistical optimization for enhanced drug encapsulation and properties evaluation

Malakouti-Nejad, Maryam; Bardania, Hassan; Aliakbari, Farhang; Baradaran‐Rafii, Alireza; Elahi, Elahe; Monti, Daniela; Morshedi, Dina

doi:10.1016/j.ijpharm.2020.119895

Cited by 17 publications

(13 citation statements)

References 78 publications

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“…Particle size is one of the most influencing factors guiding the behavior of nanomaterials, stability, release profiles, biological distribution, and cellular uptake. It has been stated that decreasing the size of nanoliposomes promotes their permeation through intercellular paths [ 19 ]. Additionally, the polydispersity index (PdI) of lipid-based nanovesicles may affect their stability and is considered a key factor in particle size distribution.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

et al. 2021

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The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil (Origanum vulgare L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration–sonication method; (2) nano-size characterization using various analytical and imaging techniques; and (3) antifungal efficacy of as-developed OEO nanoliposomes against Trichophyton rubrum (T. rubrum) by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to −36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against T. rubrum strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against T. rubrum.

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Section: Resultsmentioning

confidence: 99%

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

et al. 2021

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“…BCZ entrapment efficiency was close to 50% showing in vitro release at different proportions when surfactants were added to the medium [12.9%, sodium dodecyl sulfate; 19.4%, tween-20; 4.6%, triton X-100; and 20.6%, poly(ethylene glycol)-2000 (PEG-2000)]. Interestingly, encapsulated BCZ maintained structural stability against thermal stress (4, 24, 37, and 50 °C) [ 38 ].…”

Section: Development Of Nanoparticulate Drug Delivery Systems For Bczmentioning

confidence: 99%

“…The overexpression of VEGF has been reported as one of the contributors to several ocular diseases [ 109 ]. Aiming the intraocular drug delivery, BCZ carried in nanogel [ 63 ], liposomes [ 38 ] or albumin nanoparticles [ 57 ] demonstrated non-cytotoxicity against human-derived retinal pigment epithelial (ARPE-19) cells. The absence of a cytotoxic effect was also observed for liposomal BCZ against human umbilical vein endothelial cells (HUVEC) [ 38 ].…”

Section: Pharmacological Applicationsmentioning

confidence: 99%

“…In another study, a fatty acid with a Krafft point below 60 °C was used due to the thermosensitivity of BCZ [ 41 ]. The maintenance of BCZ structural characteristics after encapsulation in liposomes and under thermal stress was proved [ 38 ]. Trehalose was added to PLGA nanoparticles to maintain the physical and chemical stability of BCZ.…”

Section: Limitations and Advantages Of Bcz Nanocarriersmentioning

confidence: 99%

“…Therefore, these nanocarriers can decrease the multiple administrations by parenteral or intravitreal route, improving the patient compliance to treatment. Furthermore, nanoformulations with BCZ presented good tolerability [ 35 , 49 , 52 ] and hemocompatibility [ 38 , 54 , 65 , 71 ]. The intravitreal injection of nanoparticles with BCZ did not cause ocular toxicity in rabbits [ 44 ] neither in mice [ 45 ] or in rats [ 36 ].…”

Section: Limitations and Advantages Of Bcz Nanocarriersmentioning

confidence: 99%

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Organic Nanocarriers for Bevacizumab Delivery: An Overview of Development, Characterization and Applications

et al. 2021

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Bevacizumab (BCZ) is a recombinant humanized monoclonal antibody against the vascular endothelial growth factor, which is involved in the angiogenesis process. Pathologic angiogenesis is observed in several diseases including ophthalmic disorders and cancer. The multiple administrations of BCZ can cause adverse effects. In this way, the development of controlled release systems for BCZ delivery can promote the modification of drug pharmacokinetics and, consequently, decrease the dose, toxicity, and cost due to improved efficacy. This review highlights BCZ formulated in organic nanoparticles providing an overview of the physicochemical characterization and in vitro and in vivo biological evaluations. Moreover, the main advantages and limitations of the different approaches are discussed. Despite difficulties in working with antibodies, those nanocarriers provided advantages in BCZ protection against degradation guaranteeing bioactivity maintenance.

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Long-Acting Strategies for Antibody Drugs: Structural Modification, Controlling Release, and Changing the Administration Route

Wang,

Song,

et al. 2024

Eur J Drug Metab Pharmacokinet

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Formulation of nanoliposome-encapsulated bevacizumab (Avastin): Statistical optimization for enhanced drug encapsulation and properties evaluation

Cited by 17 publications

References 78 publications

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Organic Nanocarriers for Bevacizumab Delivery: An Overview of Development, Characterization and Applications

Long-Acting Strategies for Antibody Drugs: Structural Modification, Controlling Release, and Changing the Administration Route

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