Formulation of highly volatile pheromone of the white grub beetle Dasylepida ishigakiensis (Coleoptera: Scarabaeidae) to develop monitoring traps

Wakamura, Sadao; Yasui, Hiroe; Mochizuki, Fumiaki; Fukumoto, Takehiko; Arakaki, Norio; Nagayama, Atsushi; Uesato, Takumi; Miyagi, Akiko; Oroku, Hiroaki; Tanaka, Seiji; Tokuda, Makoto; Fukaya, Midori; Akino, Toshiharu; Hirai, Yoshiro; Shiga, Masakazu

doi:10.1303/aez.2009.579

Cited by 19 publications

(15 citation statements)

References 13 publications

(11 reference statements)

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“…pheromones by adding components to sunflower oil impregnated onto cellulose acetate cigarette filters. 22 Considering that PE could be used to dispense highly volatile pheromones for pest monitoring, 47 we used sealed PE vials as dispensers for the pheromone of A. lucorum, adding sunflower oil as a solvent. During our previous tests, sunflower oil performed well in stabilizing 4-OHE, which is known for its instability.…”

Section: Discussionmentioning

confidence: 99%

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Tao

Mei

Zhang

et al. 2020

Pest Management Science

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BACKGROUND The plant bug, Apolygus lucorum Meyer‐Dür, has begun a resurgence and has become a key pest in cotton in northern China, with the wide‐scale adoption of transgenic Bt cotton. We attempted to develop a new approach to the control of this plant bug by identifying and utilizing its sex pheromone. RESULTS Extracts from A. lucorum adults and nymphs were analyzed using gas chromatography–mass spectrometry (GC–MS) and gas chromatography–electroantennogram detection (GC–EAD) to identify the sex pheromone components. (E)‐4‐Oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate were the major sex pheromone components from females and were also electrophysiology‐active towards male antennae. For males, (E)‐4‐oxo‐2‐hexenal and hexyl butyrate were the major sex pheromone components. These three components, however, were not detected in nymphs. Field tests showed that binary blends of (E)‐4‐oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate at a ratio of 3:2 resulted in trapping the greatest numbers of A. lucorum males. A long‐term dispenser was developed by loading the pheromone components dissolved in sunflower oil into polyethylene vials. Further field evaluation showed that the polyethylene dispensers attracted significantly more bugs even when deployed in the field for 5 weeks. CONCLUSION We identified the sex pheromone of A. lucorum and developed a type of high‐efficiency and long‐term lure. Our results could expand knowledge of the pheromones of plant bugs, and provide novel technologies to monitor and control this pest. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Tao

Mei

Zhang

et al. 2020

Pest Management Science

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“…Adult males of D. ishigakiensis were captured by funnel vane traps (diameter 15 cm, height 39 cm; Trécé Inc., Salinas, California) with polyethylene tube lures (length 1.0–10.0 cm) containing approximately 12 mg cm −1 of ( R )‐2‐butanol (Shin‐Etsu Chemical Co., Ltd, Japan) in sugarcane fields on Miyako Island (24°47′N, 125°17′E), Okinawa, Japan, in February 2008 (Wakamura et al , 2009b) and immediately transported to the Tsukuba laboratory in Ibaraki, Japan. Upon arrival, groups of ten males were put into plastic containers (diameter 13 cm, depth 7 cm) with sterilized moist humus and maintained under an LD 12 : 12 h photocycle [lights on 08.30 h Japan Standard Time (JST)] at 16 °C for at least 3 days prior to experiments.…”

Section: Methodsmentioning

confidence: 99%

A two-step mechanism controls the timing of behaviour leading to emergence from soil in adult males of the scarab beetle Dasylepida ishigakiensis

Tokuda

Tanaka

Maeno

et al. 2010

Physiological Entomology

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Adults of the white grub beetle Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scarabaeidae) emerge from the soil around dusk for mating on subtropical islands. The present study examines the factors controlling the emergence of males in the laboratory. There are two steps involved. Standby behaviour (i.e. insect head appears at the soil surface) can be observed for several hours before the beetles actually emerge for mating. The standby behaviour is facilitated by warm conditions, although the proportion of standby individuals is influenced not only by the temperature on that day, but also by that on the previous day. Experiments in which beetles are exposed to photoperiod and thermoperiod combinations, in and out of phase, show that temperature is more important in inducing standby and emerging behaviour than light alone. For the second step, factors such as temperature, light and the presence of the female sex pheromone determine whether males will leave the standby position and emerge onto the ground. The female sex pheromone stimulates standby beetles to exhibit emerging and wing vibration behaviours, although the effect depends on when it is presented to beetles. Beetles burrow back into the soil; this behaviour is influenced by illumination and time of the day but not by temperature. The results suggest that D. ishigakiensis possesses a sophisticated mechanism controlling male emergence from the soil.

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“…Adult D. ishigakiensis used for experiments were captured in sugarcane fields on Miyako Island (24°47′N; 125°17′E), Okinawa, Japan. Virgin males were obtained using funnel traps with ( R )‐2‐butanol, which is the primary component of the female sex pheromone (Wakamura et al , 2009a, b). They were maintained together in plastic containers (35 × 25 × 11 cm) with moist leaf mould.…”

Section: Methodsmentioning

confidence: 99%

Factors influencing adult emergence from soil and the vertical distribution of burrowing scarab beetles Dasylepida ishigakiensis

Harano

Tanaka

Tokuda

et al. 2010

Physiological Entomology

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The present study investigates the emergence of adult white grub beetles Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scrabaeidae) from soil as well as their burrowing behaviours. 'Standby behaviour' (i.e. adults come to the soil surface where they expose their heads) is shown in the field and, along with emergence behaviour, is entrained by LD photocycles. These 24-h rhythms persist after transfer to continuous light conditions for 2 days. By contrast, beetles transferred from LD photocycles to continuous dark conditions fail to show standby behaviour; thus, it appears to be manifested only in the presence of illumination. Under dark conditions, beetles emerge completely from the soil directly at the time when standby behaviour is otherwise expected to occur. Emerged adults then burrow back into the soil before dawn. Virgin and mated males, as well as virgin females, which are expected to emerge from the soil for mating on later evenings, burrow to a relatively shallow depth (<2 cm), whereas mated females burrow deeper (2-10 cm). Soil properties such as moisture, grain size, topography and temperature influence the burrowing behaviour and the depths that the beetles reach.

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Formulation of highly volatile pheromone of the white grub beetle Dasylepida ishigakiensis (Coleoptera: Scarabaeidae) to develop monitoring traps

Cited by 19 publications

References 13 publications

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

A two-step mechanism controls the timing of behaviour leading to emergence from soil in adult males of the scarab beetle Dasylepida ishigakiensis

Factors influencing adult emergence from soil and the vertical distribution of burrowing scarab beetles Dasylepida ishigakiensis

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