Formulation of Herbal Gel of Antirrhinum majus Extract and Evaluation of its Anti- Propionibacterium acne Effects

Abstract

Background:Antirrhinum majus contains aurone with excellent antibacterial and antifungal activities. In addition, visible light activates the endogenous porphyrins of Propionibacterium acne, which results in bacterial death. Therefore, considering the above-mentioned facts, the aim of the present study was to prepare a topical herbal gel of A. majus hydroalcoholic extract and to evaluate its antiacne effects with or without blue light combination as an activator of the porphyrins.Materials and Methods:Antibacterial activity of the shoot or petal extracts was evaluated by disc diffusion method and the minimum inhibitory concentration (MIC) was calculated. Various gel formulations were developed by the Experimental Design software. The obtained gel formulations were prepared and tested for pharmaceutical parameters including organoleptic features, pH, viscosity, drug content, and release studies. Finally, the antibacterial activity was evaluated against (P. acnes) with or without blue light.Results:The MIC of the extracts showed to be 0.25 μg/ml. Evaluation of the gel formulation showed acceptable properties of the best formulation in comparison to a gel in the market. Pharmaceutical parameters were also in accordance with the standard parameters of the marketed gel. Furthermore, statistical analyses showed significant antibacterial effect for gel when compared to negative control. However, combination of blue light with gel did not show any significant difference on the observed antibacterial effect.Conclusion:Because of the statistically significant in vitro antiacne effects of the formulated gel, further clinical studies for evaluation of the healing effects of the prepared gel formulation on acne lesions must be performed.

The In vitro Biological Activity of Biosynthesized Silver Nanoparticles Produced Using Mangifera indica Stem Bark Extract and Properties of Its Pharmaceutical Gel Formulation

“…For the formulated gels, the results of the pH showed that the gel systems were within acceptable pH range. The gels showed a pH range of 6.8-7.1 and this is in accordance with Shahtalebi et al [44]. The effect of pH on skin infections have been studied and it has been observed that at low pH values of 4.7, Staphylococcus epidermidis shows growth, while Staphylococcus aureus is suppressed at a pH value of 7 [45].…”

BÀO CHẾ GEL RỬA MẶT TỪ KHỔ QUA (Momordica charantia L)

“…Gel rửa mặt được coi là kém chất lượng nếu lượng mực bị cô đặc trong bọt, các chất bẩn nên tồn tại trong phần nước vì nếu bụi bẩn còn sót lại trong phần bọt sẽ rất khó tẩy rửa và sẽ bám lại trên da, không làm sạch được da mặt. Yêu cầu không có mực trong bọt [9].…”

In Vivo Phytotherapy in BALB/c Athymic Nude Mice: Hair Growth Promotion using <i>Ficus religiosa</i> L. and <i>Morus alba</i> L.

“…The plants samples were identified by Botanical Survey of India (BSI), Western Regional Center, Pune, Maharashtra, India. 5% gel was prepared from aqueous fractions of Ficus religiosa L. and Morus Alba L. in combination according to modified protocol based on procedure described previously 33 . Briefly, the individual fractions were dissolved in 4 ml of distilled water followed by addition of 4% propylene glycol and 3% ethanol.…”