Formulation of Glycyrrhizic Acid-based Nanocomplexes for Enhanced Anti-cancer and Anti-inflammatory Effects of Curcumin

Song, Jae Bok; Kim, Jun Yeong; You, Gayeon; Kang, Yoon Young; Yang, Jun; Mok, Hyejung

doi:10.1007/s12257-021-0198-7

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…GA is the main active component of Glycyrrhiza uralensis Fisch (Khan et al., 2018). Numerous studies have pointed out that GA can treat cancers such as colon cancer (Zuo et al., 2022), gastric cancer (Qiao, 2022), glioblastoma (Li et al., 2014), and breast cancer (Song et al., 2022). However, the mechanism of the anti‐melanoma activity of GA is still vague.…”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin‐induced apoptosis of melanoma cells

Bian,

Niu,

et al. 2024

Chem Biol Drug Des

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This research was designed to prospect the mechanism and impact of glycyrrhizic acid (GA) on DNA damage repair and cisplatin (CP)‐induced apoptosis of melanoma cells. First, human melanoma cell SK‐MEL‐28 was stimulated using GA for 24, 48, and 72 h. Then, the optimal treatment time and dosage were selected. After that, cell counting kit‐8 (CCK‐8) was employed for testing the cell viability, flow cytometry for the apoptosis, comet assay for the DNA damage of cells, and western blot for the cleaved‐Caspase3, Caspase3, Bcl‐2, and γH2AX protein expression levels. The experimental outcomes exhibited that as the GA concentration climbed up, the SK‐MEL‐28 cell viability dropped largely, while the apoptosis level raised significantly, especially at the concentration of 100 μm. In addition, compared with GA or CPtreatment only, CP combined with GA notably suppressed the viability of melanoma cells and promoted cell apoptosis at the cytological level. At the protein level, the combined treatment notably downregulated the Bcl‐2 and Caspase3 expression levels, while significantly upregulated the cleaved‐Caspase3 and γH2AX expression levels. Besides, CP + GA treatment promoted DNA damage at the DNA molecular level. Collectively, both GA and CP can inhibit DNA damage repair and enhance the apoptosis of SK‐MEL‐28 cells, and the synergistic treatment of both exhibits better efficacy.

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Section: Discussionmentioning

confidence: 99%

Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin‐induced apoptosis of melanoma cells

Bian,

Niu,

et al. 2024

Chem Biol Drug Des

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“…[19][20][21] The liquiritin apioside (Figure 1G) in Radix Glycyrrhizae has antitussive and expectorant activities. 22 It can be deduced from the above materials that the active compounds of XBSD influence its efficacy. On the other hand, it is worth noting that glycyrrhizic acid is reported as a surfactant that can self-assembles into micelles in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembled Nanoparticles from Xie-Bai-San Decoction: Isolation, Characterization and Enhancing Oral Bioavailability

Nie,

Liu,

Lan

et al. 2024

IJN

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Background: Natural nanoparticles have been found to exist in traditional Chinese medicine (TCM) decoctions. However, whether natural nanoparticles can influence the oral bioavailability of active compounds has not been elucidated. Using Xie-Bai-San decoction (XBSD) as an example, the purpose of this study was to isolate, characterize and elucidate the mechanism of the nanoparticles (N-XBSD) in XBSD, and further to explore whether the bioavailability of the main active compounds could be enhanced by N-XBSD. Methods: N-XBSD were isolated from XBSD, and investigated its characterization and study of its formation mechanism, and evaluation of its ability to enhance bioavailability of active compounds. Results: The N-XBSD was successfully isolated with the average particle size of 104.53 nm, PDI of 0.27 and zeta potential of −5.14 mV. Meanwhile, all the eight active compounds were most presented in N-XBSD. Kukoamine B could self-assemble with mulberroside A or liquiritin to form nanoparticles, respectively. And the FT-IR and HRMS results indicated the possible binding of the ammonium group of kukoamine B with the phenolic hydroxyl group of mulberroside A or liquiritin, respectively. The established UPLC-MS/MS method was accurate and reliable and met the quantitative requirements. The pharmacokinetic behaviors of the N-XBSD and decoction were similar in rats. Most notably, compared to that of free drugs, the C max , AUC 0-∞ , AUC 0-t , T 1/2 and MRT 0-∞ values of index compounds were the higher in N-XBSD, with a slower plasma clearance rate in rats. Conclusion:The major active compounds of XBSD were mainly distributed in N-XBSD, and N-XBSD was formed through selfassembly among active compounds. N-XBSD could obviously promote the bioavailability of active compounds, indicating natural nanoparticles of decoctions play an important role in therapeutic effects.

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“…As a result, there is increasing attention to polysaccharides in the field of molecular therapeutics. Prior studies have demonstrated that water-soluble polysaccharides extracted from Chinese herbal medicines provide medicinal benefits such as immune regulation, antioxidation, antitumor activity, liver protection, and anticoagulation (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory and antioxidant effects of Glycyrrhiza uralensis polysaccharide in Lohmann Brown chickens

et al. 2022

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Glycyrrhiza polysaccharide extract 1 (GPS-1) is a bioactive component isolated from Glycyrrhiza uralensis, also known as Chinese licorice. It appears to be pharmacologically active as an antibacterial, antiviral, and anti-tumor agent. GPS-1 has also been shown to buffer liver health and regulate the immune system. Moreover, GPS-1 is low cost and easy to extract. More study was needed to elucidate the biochemical pathways underlying the immunomodulatory and antioxidant benefits observed in Glycyrrhiza polysaccharide extract 1 (GPS-1). in vitro experiments on chicken lymphocytes and dendritic cells (DCs) show that GPS-1 significantly promotes the proliferation of immune cells and is linked to lymphocytes' secretion of IL-12, IFN-γ, and TNF-α by. DC secretion of NO, IL-2, IL-1β, IFN-γ, TNF-α, and IL-12p70 was also increased significantly. Additionally, GPS-1 also displayed a significant antioxidant effect in vitro, able to scavenge DPPH, hydrogen peroxide, ABTS, and other free radicals like superoxide anions. Separately, GPS-1 was tested in vivo in combination with the Newcastle disease virus (NDV) – attenuated vaccine. 120 Lohmann Brown chickens were vaccinated, while another 30 became the unvaccinated blank control (BC) group. For three consecutive days 1 mL of GPS-1 was administered at doses of 19.53 μg/mL, 9.77 μg/mL, or 4.88 μg/mL to the ND-vaccinated birds, except for the vaccine control (VC), where n = 30 per group. In vivo results show that GPS-1 combined with Newcastle disease (ND) vaccine had the best efficacy at significantly increasing chickens' body weight and ND serum antibody titer, enhancing their secretion of IL-2 and IFN- γ, and promoting the development of immune organs. The results also indicate that GPS-1 was able increase the proliferation of in vitro immune cells and elevate their cytokine secretion, which enhances the body's immune response. GPS-1 also clearly has the potential to be used as an immune adjuvant alongside ND vaccination.

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Formulation of Glycyrrhizic Acid-based Nanocomplexes for Enhanced Anti-cancer and Anti-inflammatory Effects of Curcumin

Cited by 11 publications

References 36 publications

Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin‐induced apoptosis of melanoma cells

Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin‐induced apoptosis of melanoma cells

Self-Assembled Nanoparticles from Xie-Bai-San Decoction: Isolation, Characterization and Enhancing Oral Bioavailability

Immunomodulatory and antioxidant effects of Glycyrrhiza uralensis polysaccharide in Lohmann Brown chickens

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