Formulation development, optimization and evaluation of aloe vera gel for wound healing

Khan, Abdul Wadood; Kotta, Sabna; Ansari, S. H.; Sharma, Rakesh Kumar; Kumar, Amit; Ali, Javed

doi:10.4103/0973-1296.117849

Cited by 75 publications

(33 citation statements)

References 11 publications

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“…It is the one of oldest medicinal substances that was used in wound healing [12]. AV has a potentially significant anti-inflammatory effect, therefore it could be used in treating: gingivitis [12][13][14], skin wound [15][16][17] and first to second degree burns [18,19]. Topical application of AV has been demonstrated to have a promising effect on the wound healing process [15].…”

Section: Doi: 107243/2055-091x-2-3mentioning

confidence: 99%

Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study

Hamid¹,

Soliman²

2015

J Histol Histopathol

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Background: Skin burn is common universal problem that may lead to ugly scarring, serious handicapping. Availability of natural biological treatment to improve its outcome becomes concern of many researchers. Aim of study: To explore the effect of aloe vera on the angiogenesis process during healing of a fullthickness experimental skin burn. Materials and methods: Seventy rats were divided into three groups; control group (A), group B and group C. Group B was exposed to full-thickness (FT) skin burn. Group C was exposed to FT skin burn with application of local aloe vera (AV) gel. Each of group B and C was divided into 3 subgroups from which skin specimens were taken at 4,8,12 days. Skin specimenswere prepared for histological and immunohistochemical study by hematoxlyin and eosin (H&E), Masson`s trichrome (MT) and alpha smooth muscle actin (α-SMA). All data were measured morphometrically and statistically analyzed. Results: After 4 days from inducing FT burn, the initial necrosis and inflammation gradually replaced by increased granulation tissue (GT) on 8 th and 12 th day skin specimens. The collagen deposition of GT increased progressively to make a coarse dense bundles, meanwhile the newly formed capillaries (NFC) of GT were surrounded by pericytes that showed extensive expression of α-SMA early on 4 th and till 8 th day specimens and decreased on 12 th day specimens. AV-treated groups showed relative decrease of α-SMA detection especially in 8 th and 12 th day specimens together with a significant decrease in the inflammatory infiltrate in all phases as well as deposition of more mature and finer collagen fibers compared with burn per se specimens. Conclusion: In full-thickness skin burn, aloe vera shows a beneficial effect by reducing the inflammation significantly and providing a more mature granulation tissue which could accelerate healing and might produce a sound well-remodeled scar.

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Section: Doi: 107243/2055-091x-2-3mentioning

confidence: 99%

Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study

Hamid¹,

Soliman²

2015

J Histol Histopathol

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“…The decrease in the wound area was monitored at specific time intervals. The wound contractions were measured by using the standard method of tracing a wounded margin on a tracing paper[ 38 ] and calculating the percentage reduction in the wound area. Area of the wound at day zero was considered as 100% to calculate the percentage reduction in the wound area.…”

Section: Methodsmentioning

confidence: 99%

Optimization, characterization, and efficacy evaluation of 2% chitosan scaffold for tissue engineering and wound healing

et al. 2016

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Objective:To develop a chitosan-based scaffold and carry out a complete comprehensive study encompassing optimization of exact chitosan strength, product characterization, toxicity evaluation, in vitro validation in cell culture experiments, and finally in vivo efficacy in animal excision wound model.Materials and Methods:Developed chitosan scaffolds (CSs) were optimized for tissue engineering and wound healing efficacy by means of microstructure, toxicity, and biocompatibility evaluation.Results:Scanning electron microscope (SEM) studies revealed that porosity of CS decreased with increase in chitosan concentration. Chemical stability and integrity of scaffolds were confirmed by Fourier transform infrared studies. Highest swelling percentage (SP) of 500% was observed in 2%, while lowest (200%) was observed in 1% CS. Reabsorption and noncytotoxic property of optimized scaffold were established by enzymatic degradation and MTT assay. Enzymatic degradation suggested 20–45% of weight loss (WL) within 14 days of incubation. Cytotoxicity analysis showed that scaffolds were noncytotoxic against normal human dermal fibroblast human dermal fibroblast cell lines. Significant cellular adherence over the scaffold surface with normal cellular morphology was confirmed using SEM analysis. In vivo efficacy evaluation was carried out by means of reduction in wound size on Sprague-Dawley rats. Sprague-Dawley rats treated with optimized scaffold showed ~ 100% wound healing in comparison to ~80% healing in betadine-treated animals within 14 days. Histological examination depicted advance re-epithelization with better organization of collagen bundle in wound area treated with 2% CS in comparison to conventional treatment or no treatment.Conclusion:This study, thus, reveals that 2% CSs were found to have a great potential in wound healing.

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“…Wound healing is a complex and dynamic process that involves a cascade of cellular reactions to restore cellular structures and tissue layers. Aloe vera gel has been known to accelerate wound healing [122,123,124], and protect and soothe the skin tissues [125]. The glucomannan, a water soluble polysaccharide in aloe vera, affects fibroblast growth factor and stimulates the activity and proliferation of these cells and, finally improves collagen production and secretion [126].…”

Section: Aloe Vera Based Gels and Filmsmentioning

confidence: 99%

Aloe Vera for Tissue Engineering Applications

Rahman

Carter

Bhattarai

2017

JFB

154

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Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

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Formulation development, optimization and evaluation of aloe vera gel for wound healing

Cited by 75 publications

References 11 publications

Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study

Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study

Optimization, characterization, and efficacy evaluation of 2% chitosan scaffold for tissue engineering and wound healing

Aloe Vera for Tissue Engineering Applications

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