Formulation and utilisation of spent anaerobic digestate fluids for the growth and product formation of single cell algal cultures in heterotrophic and autotrophic conditions

Silkina, Alla; Zacharof, Myrto-Panagiota; Hery, Guillaume; Nouvel, Thibaud; Lovitt, Robert W.

doi:10.1016/j.biortech.2017.05.133

Cited by 28 publications

(25 citation statements)

References 39 publications

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“…The initial salinity concentration for all experiments was 30 IU by adding seawater salt to the wastewater. The injected inoculum of culture (in exponential growth) was 10% of the total volume (150 L) with cell concentration~54.1 × 10 6 Cell mL −1 ; control medium was Cell-Hi F2P from Varicon aqua readymade media powder (http://www.variconaqua.com/products-services/algal-nutrients-andculture-media/) [23]. Waste-based media was prepared by diluting the waste stream.…”

Section: Experimental Designmentioning

confidence: 99%

“…The pretreatment of this waste consisted of dilution, mixing, sedimentation and sieving in a 200 L stainless steel vessel. The resulting effluents were then microfiltered using a pilot scale unit equipped with a ceramic membrane (pore size < 0.2 µm) [23]. The resulting particle-free effluents were used as feedstock for microalgae cultivation.…”

Section: Experimental Designmentioning

confidence: 99%

“…For this reason, Nannochloropsis oceanica (N. oceanica) was the model species in this study. This marine species can grow in seawater from the ocean [23] or waste brine water [9,24]. Brine (highly concentrated NaCl water) is the main residue produced during the desalination process through reverse osmosis.…”

Section: Introductionmentioning

confidence: 99%

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Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

et al. 2019

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Municipal and agricultural waste treatment is one of the key elements of reducing environmental impact with direct effects on the economy and society. Algal technology has been tested to enable effective recycling and valorisation of wastewater nutrients including carbon, nitrogen and phosphorus. An integrated evaluation and optimisation of the sustainability of an algal bio-refinery, including mass and energy balances, carbon, water and nutrient use and impact analysis, was assessed. A bio-refinery approach of waste remediation using algal cultivation was developed at Swansea University, focusing on nutrient recovery via algal biomass exploitation in pilot facilities. Mass cultivation (up to 1.5 m3) was developed with 99% of nitrogen and phosphorus uptake by microalgal cultures. Nannochloropsis oceanica was used as a biological model and grown on three waste sources. The compounds obtained from the biomass were evaluated for animal feed and as a potential source of energy. The bioremediation through algal biotechnology was examined and compared to alternative nutrient recovery passive and active methods in order to know the most efficient way of excess nutrient management. Conclusions emphasise the high potential of algal biotechnology for waste remediation and nutrients recovery, despite the need for further development and scalable applications of this new technology.

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

et al. 2019

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“…Membrane Technology (MT) is scalable with numerous arrangements and alternatives and easy to incorporate and integrate into waste treatment processes. It offers low operational cost (OPEX) compared to other competing technologies since there is no phase change required and minimal or no use of chemical additives [ 20 , 21 ]. Using this technology, waste can be recycled back to the production systems substituting for newly manufactured materials.…”

Section: Introductionmentioning

confidence: 99%

Comparing Nutrient Removal from Membrane Filtered and Unfiltered Domestic Wastewater Using Chlorella vulgaris

Mayhead

Silkina

Llewellyn

et al. 2018

Biology

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The nutrient removal efficiency of Chlorella vulgaris cultivated in domestic wastewater was investigated, along with the potential to use membrane filtration as a pre-treatment tool during the wastewater treatment process. Chlorella vulgaris was batch cultivated for 12 days in a bubble column system with two different wastewater treatments. Maximum uptake of 94.18% ammonium (NH4-N) and 97.69% ortho-phosphate (PO4-P) occurred in 0.2 μm membrane filtered primary wastewater. Membrane filtration enhanced the nutrient uptake performance of C. vulgaris by removing bacteria, protozoa, colloidal particles and suspended solids, thereby improving light availability for photosynthesis. The results of this study suggest that growing C. vulgaris in nutrient rich membrane filtered wastewater provides an option for domestic wastewater treatment to improve the quality of the final effluent.

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“…Conversely, anaerobic digestates from other feedstocks have shown to be less efficient as algae culture medium in comparison to commercial media (Silkina et al, 2017. Besides the higher amount of soluble phosphorous available, algal digestates have the advantage of a higher level of magnesium which is a main component of chlorophyll molecules and therefore an essential macronutrient for microalgae .…”

Section: Algal Growth In Liquid Anaerobic Digestatementioning

confidence: 99%

Development of a closed loop microalgal oil production system through anaerobic digestion and nutrient recycling

Gonzalez¹

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The world needs new energy sources that give adequate yields at low economic and environmental costs. Biofuels such as biodiesel and biogas are viable options to offset usage of traditional fossil fuels and reduce environmental damage. In this context, microalgae are promising organisms for biodiesel production due to their capacity to accumulate significant amounts of lipids. Additionally, anaerobic digestion is becoming an efficient and widely utilized system for the generation of renewable biogas; and microalgae are a suitable substrate for such purpose. To date, research on microalgal biofuels has been performed mainly at a pilot-scale due to economic considerations and, in some cases, negative energy balances of the processes or potentially unsustainable practices. For example, a large amount of mineral fertilizer is often used to mass-produce microalgae. More research is required to make biofuel production more sustainable and suitable at large scale. An attractive way to overcome these issues is to develop an integrated production system with minimal inputs to reduce the environmental cost of production by recycling nutrients. This can be achieved through the anaerobic digestion of algal biomass. The anaerobic digestion process not only produces biogas but also allows nutrient recycling for repeat algae cultivation. On the other hand, lipid extraction for biodiesel production leaves a residue that is a potential substrate for anaerobic digestion. Lipid-extracted biomass may have an advantage over pure algal cultures of a higher native digestibility due to cell disruption during the lipid extraction process. Hence, an integrated system of biodiesel and biogas production using anaerobic digestion effluent as a nutrient source for algae growth and lipid-extracted biomass as substrate for digestion may present a good option. This thesis focused on the optimization of a closed nutrient loop for oil and biogas production through anaerobic digestion of microalgae. The study was oriented towards the scalability of the system aiming to optimize the main aspects that currently jeopardize the application of the proposed biorefinery at large scale. To address these issues three main aspects were investigated: (1) Wet and solvent-free lipid extraction, (2) Osmotic shock as pre-treatment for lipid extraction and biogas production and (3) Nutrient recycling for algae growth. This dissertation has been structured as follows: Chapter 1 is a literature review of the system proposed. Chapter 2 evaluates the anaerobic digestion of the microalga Scenedesmus dimorphus under continuous conditions as well as the potential of the anaerobic digestate as culture medium for the same species. In chapter 3 the feasibility of a wet solvent-free lipid extraction procedure by using osmotic shock is evaluated as pre-treatment. The species Dunaliella salina and Chaetoceros muelleri were chosen for this purpose as they have very fragile cell wall. Besides, the methane Publications included in this thesis GONZÁLEZ-GONZÁLEZ, L. M., ELTANAHY, E...

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Formulation and utilisation of spent anaerobic digestate fluids for the growth and product formation of single cell algal cultures in heterotrophic and autotrophic conditions

Cited by 28 publications

References 39 publications

Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

Comparing Nutrient Removal from Membrane Filtered and Unfiltered Domestic Wastewater Using Chlorella vulgaris

Development of a closed loop microalgal oil production system through anaerobic digestion and nutrient recycling

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