Formulation and testing of vancomycin loaded albumin microspheres prepared by spray-drying

Nettey, Henry; Haswani, Dinesh; Oettinger, Carl W.; D’Souza, Martin J.

doi:10.1080/02652040600776564

Cited by 26 publications

(17 citation statements)

References 11 publications

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“…Seven formulations (A to G, Table 1) containing different amounts of PEG ranging from 0.0-66.7% (w/w, relative to BSA) were prepared in 5% BSA (w/v) and cross-linked by glutraldehyde for 18 hrs at room temperature as described previously [1][2][3][4][5][6][7]. Excess glutraldehyde was neutralized with sodium bisulfite.…”

Section: Preparation Of Microspheresmentioning

confidence: 99%

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Novel approach for preparing nontoxic stealth microspheres for drug delivery

Angra

Rizvi

Oettinger³

et al. 2011

Eur. J. Chem.

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Section: Preparation Of Microspheresmentioning

confidence: 99%

“…Cross-linked albumin microspheres are a potential delivery method for slow release of drugs or antigens to the targeted sites [1][2][3][4][5][6][7]. Bovine serum albumin microspheres have been extensively investigated in controlled release drug delivery systems since introduced by Kramer [8].…”

Section: Introductionmentioning

confidence: 99%

Novel approach for preparing nontoxic stealth microspheres for drug delivery

Angra

Rizvi

Oettinger³

et al. 2011

Eur. J. Chem.

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“…rats were divided into fifteen groups randomly. [15,16] Collected blood samples were immediately centrifuged at 8000 Â g for 10 min to separate plasma and stored at -20 º C until assay. Samples were assayed for PM content by HPLC method given earlier.…”

Section: Pharmacokinetics Evaluation Of Pmmentioning

confidence: 99%

Bioanalytical method development, pharmacokinetics, and toxicity studies of paromomycin and paromomycin loaded in albumin microspheres

Khan

Sharma

Kumar

2012

Drug Testing and Analysis

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Intracellular location of leishmania parasite in macrophages protects them from both hosts defence system as well as from antibiotics like paromomycin (PM) acting against them, thus there is a need of a formulation targeting intracellular parasites. Considering this, PM-loaded albumin microspheres (PM-MS) were prepared to target PM to macrophages where leishmania parasites resides and evaluated for their safety profile. A new bioanalytical method for quantitative determination of PM in rat plasma was developed by pre-column derivatization with 9-fluorenylmethyl chloroformate. The developed bioanalytical method was validated and applied for pharmacokinetic studies of PM administered by intramuscular and intravenous routes as well as for developed PM-MS which were administered by intravenous route. Comparative acute and subacute toxicity studies were also carried out for these formulations. The developed method was found to be very sensitive with a quantification limit of 40 ng/ml. Pharmacokinetic studies demonstrated nearly 80% reduction in C(max) of PM when administered as PM-MS, compared to other formulations at equivalent dose. Toxicity studies indicated increased level of blood urea and blood urea nitrogen in PM intramuscular injection at 90 mg/kg dose, whereas at the same dose level PM-MS showed no symptoms of toxicity. Results obtained suggest that developed PM-MS formulation is a promising alternative to the presently marketed PM intramuscular injection for the treatment of visceral leishmaniasis.

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“…[12][13][14] Thermal cross-linking was carried out at 120 º C for different time periods (12 and 24 h). Two methods of cross-linking -(1) chemical cross-linking using glutaraldehyde, and (2) thermal cross-linking using heat treatment at high temperatureswere used in present experiments.…”

Section: Thermal/chemical Cross-linking Of Microspheresmentioning

confidence: 99%

Paromomycin‐loaded albumin microspheres: Efficacy and stability studies

Khan

Kumar

Singh

et al. 2012

Drug Testing and Analysis

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In the present work, paromomycin-loaded albumin microspheres (PM-MS) have been formulated for passive targeting of paromomycin (PM) to macrophages, for the treatment of visceral leishmaniasis (VL). PM-MS were prepared by spray-drying method with a mean particle size of ≈ 3 µm. Thermal and chemical cross-linking methods were used for controlling drug release from the prepared microspheres (MS). PM-MS were then tested for efficacy and stability studies. In efficacy study, in vitro promastigote assay was carried out to assess the susceptibility of promastigote to PM in the concentration range of 5.0-150 µg/ml; cytotoxicity assay was performed to determine possible toxicity of PM for the host cells (peritoneal macrophages) and intracellular amastigote assay was carried out to determine the efficacy of free PM (PM solution) and encapsulated PM (PM-MS). Results obtained indicated a significant increase in efficacy of PM-MS in comparison to PM solution at equivalent concentration. Subsequently, stability studies of prepared formulation was carried out at various temperature and humidity conditions, these studies provided stability of formulation at all tested conditions including accelerated conditions. Thus, it can be concluded that present work provides an optimized formulation with stability and enhanced efficacy.

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Formulation and testing of vancomycin loaded albumin microspheres prepared by spray-drying

Cited by 26 publications

References 11 publications

Novel approach for preparing nontoxic stealth microspheres for drug delivery

Novel approach for preparing nontoxic stealth microspheres for drug delivery

Bioanalytical method development, pharmacokinetics, and toxicity studies of paromomycin and paromomycin loaded in albumin microspheres

Paromomycin‐loaded albumin microspheres: Efficacy and stability studies

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