Formulation and evaluation of albumin microspheres and its enteric coating using a spray-dryer

Bejugam, Naveen K.; Uddin, Akm N.; Gayakwad, Sanjay G.; D’Souza, Martin J.

doi:10.1080/02652040802476187

Cited by 27 publications

(8 citation statements)

References 8 publications

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“…increase in PI of 63.99 ± 22.42%. According to Bejugam et al (2008), the particle size distributions of the enteric surface-coated particles are potentially influenced by the presence of other types of particles along with the targeted surface-coated particles. In other words, the enteric surface-coated dispersions may include small percentages of only drug nanoparticles, only Eudragit Õ L100-55 nanoparticles as well as drug-Eudragit Õ L100-55 nanoparticles.…”

Section: In Vitro Characterization Of Enteric Surface-coated Cubosomamentioning

confidence: 99%

“…In fact, the design of promising enteric surface-coated carriers has gained considerable attention in the last years (Chandy et al, 2002;Eerikäinen & Kauppinen, 2003;Bejugam et al 2008;Zhang et al, 2011;Hao et al, 2013). Yet, most of the reported methods suffer from many limitations including the lengthy production steps, the use of organic solvents, the need of special sophisticated equipment and/or the limited scaling up possibilities.…”

Section: Introductionmentioning

confidence: 99%

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Duodenum-triggered delivery of pravastatin sodium: II. Design, appraisal and pharmacokinetic assessments of enteric surface-decorated nanocubosomal dispersions

et al. 2016

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Context: Pravastatin sodium (PVS) is a freely water-soluble HMG-CoA inhibitor that suffers from instability at gastric pH, extensive first pass metabolism, short elimination half-life (1-3 h) and low oral bioavailability (18%). Objective: To overpower these drawbacks and to maximize drug absorption at its main site of absorption at the duodenum, enteric surface-coated PVS-loaded nanocubosomal dispersions were presented. Materials and methods: Glyceryl monooleate (GMO)-based dispersions were developed by the fragmentation or the liquid precursor methods using Pluronic Õ F127 or Cremophor Õ EL as surfactants. As a challenging entericcoating approach, the promising dispersions were surface-coated via lyophilization with Eudragit Õ L100-55; a duodenum-targeting polymer. The drug content, particle size, zeta potential, morphology and release studies of PVS-loaded dispersions were evaluated before and after surface-coating. Compared to an aqueous PVS solution, the pharmacokinetics of the best achieved system (E-F8) was evaluated (UPLC-MS/MS) in rats. Results: The enteric surfacecoated nanocubosomal dispersions were more or less spherical in shape and showed high drug-loading, negative zeta potential values and fine-tuned biphasic drug-release patterns characterized by retarded (2 h) and sustained (10 h) phases in pH 1.2 and pH 6.8, respectively. E-F8 system showed significantly (p50.05) higher oral bioavailability, delayed T max and prolonged MRT 0À1 following oral administration in rats. Conclusions: The duodenum-triggering potential and the controlled-release characteristics of the best achieved system for smart PVS delivery were revealed.

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Section: In Vitro Characterization Of Enteric Surface-coated Cubosomamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Duodenum-triggered delivery of pravastatin sodium: II. Design, appraisal and pharmacokinetic assessments of enteric surface-decorated nanocubosomal dispersions

et al. 2016

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“…28,36,37 Briefly, 500 µL of buffer A (50 mM Tris-HCl, pH 8.0, 10 mM EDTA) was added to 10 mg of magnetic microspheres and vortexed at regular interval for 30 minutes. After two hours of incubation at room temperature, 500 µL of buffer B (200 mM NaOH, 1% sodium dodecyl sulfate, 0.2 M ascorbic acid) was added to the suspension and vortexed.…”

Section: Determination Of Iron Oxide and Drug Content In Magnetic Micmentioning

confidence: 99%

Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy

Enriquez¹,

Rizvi²,

D’Souza³

et al. 2013

IJN

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Enhanced and targeted drug delivery using biodegradable microspheres is emerging as a promising approach for cancer therapy. The main objective of the present research was to formulate, characterize, and evaluate iron oxide (magnetic) containing a bovine serum albumin-based microsphere drug delivery system, capable of efficiently delivering sulforaphane, a histone deacetylase inhibitor, for an extended period of time in vivo. Magnetic microspheres were prepared by spray-drying and characterized for their physicochemical properties and dissolution profile. Further, they were evaluated for therapeutic efficacy in in vitro and in vivo systems. In vitro studies in B16 melanoma cells revealed that there was about 13%-16% more inhibition of cell viability when either 30 µM or 50 µM of sulforaphane was used with iron oxide in the polymeric carrier. Data from in vivo studies in C57BL/6 mice revealed that the magnetic microspheres (localized to the tumor site with the help of a strong magnet) inhibited 18% more tumor growth as compared with sulforaphane in solution. In addition, there was a 40% reduction in histone deacetylation levels in mice treated with iron oxide microspheres containing sulforaphane. Thus, magnetic microspheres are shown to be an effective drug delivery system for anticancer drugs.

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“…The biodegradable nanoparticles were prepared by the previously established method by our laboratory using the Buchi Mini Spray Dryer B-191 (9,10,13,27). Briefly, a 1% solution of sterile BSA in sterile water was prepared and kept for overnight crosslinking with glutaraldehyde.…”

Section: Preparation Of Nanoparticles Using Bsamentioning

confidence: 99%

“…We demonstrated that these vaccine-loaded nanoparticles are taken up by antigen-presenting cells and induced a robust innate immune response, a prerequisite for inducing adaptive immunity (9). The novel vaccine nanoparticle formulation has many advantages since it does not require chemical conjugation to a protein carrier, biodegradable, slowly release the antigen, highly immunogenic, inexpensive, easy to manufacture on large scale, highly stable, and does not require the cold chain in the logistics of vaccine delivery which reduce the cost and increase the availability (10)(11)(12). Furthermore, these vaccine nanoparticles designed for oral delivery via the mucosal route help induce an optimal immune response.…”

Section: Introductionmentioning

confidence: 99%

Induction of Death Receptor CD95 and Co-stimulatory Molecules CD80 and CD86 by Meningococcal Capsular Polysaccharide-Loaded Vaccine Nanoparticles

Ubale

Gala

Zughaier

et al. 2014

AAPS J

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ABSTRACT. Neisseria meningitidis is a leading cause of bacterial meningitis and sepsis, and its capsular polysaccharides (CPS) are a major virulence factor in meningococcal infections and form the basis for serogroup designation and protective vaccines. We formulated a novel nanovaccine containing meningococcal CPS as an antigen encapsulated in albumin-based nanoparticles (NPs) that does not require chemical conjugation to a protein carrier. These nanoparticles are taken up by antigen-presenting cells and act as antigen depot by slowly releasing the antigen. In this study, we determined the ability of CPS-loaded vaccine nanoparticles to induce co-stimulatory molecules, namely CD80, CD86, and CD95 that impact effective antigen presentation. Co-stimulatory molecule gene induction and surface expression on macrophages and dendritic cells pulsed with meningococcal CPS-loaded nanoparticles were investigated using gene array and flow cytometry methods. Meningococcal CPS-loaded NP significantly induced the surface protein expression of CD80 and CD86, markers of dendritic cell maturation, in human THP-1 macrophages and in murine dendritic cells DC2.4 in a dose-dependent manner. The massive upregulation was also observed at the gene expression. However, high dose of CPSloaded NP, but not empty NP, induced the expression of death receptor CD95 (Fas) leading to reduced TNF-α release and reduction in cell viability. The data suggest that high expression of CD95 may lead to death of antigen-presenting cells and consequently suboptimal immune responses to vaccine. The CPSloaded NP induces the expression of co-stimulatory molecules and acts as antigen depot and can spare antigen dose, highly desirable criteria for vaccine formulations.

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Formulation and evaluation of albumin microspheres and its enteric coating using a spray-dryer

Cited by 27 publications

References 8 publications

Duodenum-triggered delivery of pravastatin sodium: II. Design, appraisal and pharmacokinetic assessments of enteric surface-decorated nanocubosomal dispersions

Duodenum-triggered delivery of pravastatin sodium: II. Design, appraisal and pharmacokinetic assessments of enteric surface-decorated nanocubosomal dispersions

Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy

Induction of Death Receptor CD95 and Co-stimulatory Molecules CD80 and CD86 by Meningococcal Capsular Polysaccharide-Loaded Vaccine Nanoparticles

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