Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma

Abstract

The present research work was proposed to develop a pH triggered in-situ gel formulation for the ocular delivery of acetazolamide to treat glaucoma. Carbopol-934, Carbopol 940, Hydroxypropyl methylcellulose (HPMC) K4M, and Sodium Alginate were chosen for the development of in-situ gel. Formulation with Carbopol 940 and sodium alginate was optimized by assessing the gelling capacity. The prepared in-situ gel formulations were thoroughly characterized for gelling time, gelation temperature, Fourier-transform infrared (FTIR) spectroscopy studies, rheological study, sterility testing, corneal drug permeation, ocular irritation test, and accelerated stability study. Ex-vivo corneal permeation study was performed using goat cornea. The result of transcorneal permeation of acetazolamide followed the Fickian diffusion process. The optimized formulations showed satisfactory gelling time (4.17-5.17 sec) and dissolution time (120 min). FTIR study confirms the compatibility between the polymers and acetazolamide. The sterility study showed a satisfactory result. After 72 h observation in the in-vivo rabbit eye irritation study, the eyes appeared normal. There were no significant changes in pH and drug content in the accelerated stability studies of the formulations.

Evaluation of the Physicochemical, Antimicrobial and in vivo Wound Healing Properties of Castor Oil-Loaded Nanogels

“…21,22 pH The pH values of 1% aqueous solutions of the nanogels were measured using a digital pH metre. 23,24 Homogeneity A little portion of the sample was felt between the thumb and the index finger to determine its homogeneity. 25 Ease of removal About 1 g of each nanogel formulation was spread on the skin and placed under running water to determine its ease of removal from the skin.…”