Formulating Poly(Lactide-Co-Glycolide) Particles for Plasmid DNA Delivery

Abbas, Aiman O.; Donovan, Maureen D.; Salem, Aliasger K.

doi:10.1002/jps.21215

Cited by 70 publications

(70 citation statements)

References 99 publications

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“…[1][2][3] Successful application of gene therapy is dependent on optimization of the delivery carrier. [2][3][4][5][6] Gene therapy can be achieved using viral or non-viral vectors. [7,8] Viral vectors generate significantly higher transgene expression than non-viral vectors.…”

Section: Introductionmentioning

confidence: 99%

“…These include the requirement of cell mitosis for most retroviruses, immunogenicity of adenoviruses, safety concerns with HIV-like viruses and packaging constraints of adenoassociated viruses (AAV). [4] Promising results with some viral vectors such as AAV have progressed into human clinical trials, but the long-term safety concerns of viral vectors still exist and therapeutic levels of expression remain transient in the clinic. [9,10] In contrast, nonviral vectors display substantially reduced immunogenicity.…”

Section: Introductionmentioning

confidence: 99%

“…Advantages to non-viral vectors include ease of scale-up, storage stability, and improved quality control. [4] One of the most efficient non-viral vectors in vitro and in vivo is the cationic polymer, polyethylenimine (PEI). [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] PEI is a water-soluble polymer in which the repeat unit of PEI is two carbon atoms followed by a nitrogen atom.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Intra¹,

Salem²

2008

Journal of Controlled Release

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125

119

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Polyethylenimine (PEI) is a cationic polymer that has shown significant potential for delivering genes in vitro and in vivo. Mixing cationic PEI with negatively charged plasmid DNA (pDNA) results in the spontaneous electrostatic formation of stable nanoparticle complexes. The structure of PEI can be branched or linear. In this study, we show that branched PEI has a stronger electrostatic interaction with pDNA than linear PEI, which accounts for greater compaction, higher zeta potentials and smaller nanoparticle sizes at equivalent pDNA concentrations. For both linear and branched PEI, increasing the concentration of pDNA mixed in the same volume and at the same nitrogen to phosphate (N:P) ratio results in larger average particle sizes. Increasing the N:P ratio increases luciferase activity generated by branched PEI-pDNA nanoparticles and linear PEI-pDNA nanoparticles in HEK293, COS7 and HeLa cell lines. Increasing the N:P ratio at which branched PEI-pDNA nanoparticles are prepared also increases luciferase expression in HepG2 cells but does not increase luciferase expression generated by linear PEI-pDNA nanoparticles. In all of the cell lines, branched PEI-pDNA nanoparticles prepared at N:P ratios of 10 and above generated significantly higher luciferase activity than linear PEI-pDNA nanoparticles. Luciferase activity was highest in the HEK293 cells and luciferase expression in each of the cell lines followed the order of HEK293

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Intra¹,

Salem²

2008

Journal of Controlled Release

Self Cite

125

119

View full text Add to dashboard Cite

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“…15 In recent years, scores of people pay close attention to DNA vaccines as an emerging vaccines, 16 which can induce perfect mucosal, and humoral immune responses. 17,18 …”

Section: Newcastle Disease Virus Vaccinementioning

confidence: 99%

Newcastle disease virus vaccine encapsulated in biodegradable nanoparticles for mucosal delivery of a human vaccine

Sun¹,

Zhang

Shi

et al. 2014

Human Vaccines & Immunotherapeutics

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A n overwhelming number of medicines on the market are oral medicine with the disadvantage of lower bioavailability universally. Newcastle disease (ND) has become a serious disease that threatens the poultry industries in many countries, and there are no treatments available for ND. The biodegradable materials could be surface modified and protect antigen or DNA from damage. Furthermore, nanoparticles are also a potential drug delivery with proper size. However, Newcastle disease virus (NDV) vaccines encapsulated in nanoparticles were widely used due to their proved a high safety and induced quicker and better mucosal and humoral immune responses. Here we review the results of mucosal immune delivery system for ND. Due to the safety, low toxicity, and better immunogenicity of the mucosal immune delivery system, our studies provide a clearly view that used the biodegradable materials to research and develop the human vaccines to save more patients' lives. These promising results provide a foundation for testing the approach in humans.

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“…Currently, DNA vaccines are administered in the form of either aqueous solutions or lyophilized powders. However, because of their high hydrophilicity and therefore a low distribution coefficient between oil and water phases, 9,10 it is difficult for DNA vaccines to transfer across cell membranes after intramuscular injection, limiting the amount of vaccine to be captured by antigen-presenting cells to induce immune responses. 11 Other factors that limit the clinical applications of these novel DNA vaccines include the difficulties associated with DNA stability and delivery, resulting in low levels of DNA vaccine expression and weak immune responses, [12][13][14] especially in large animal models.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine

Zhao¹,

Rong²,

Guo³

et al. 2015

IJN

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Layered double hydroxide (LDH)@SiO 2 nanoparticles were developed as a delivery carrier for the plasmid DNA expressing the Newcastle disease virus F gene. The LDH was hydrotalcite-like materials. The plasmid DNA encapsulated in the LDH@SiO 2 nanoparticles (pFDNA-LDH@SiO 2 -NPs) was prepared by the coprecipitation method, and the properties of pFDNA-LDH@SiO 2 -NPs were characterized by transmission electron microscopy, zeta potential analyzer, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The results demonstrated that the pFDNA-LDH@SiO 2 -NPs had a regular morphology and high stability with a mean diameter of 371.93 nm, loading capacity of 39.66%±0.45%, and a zeta potential of +31.63 mV. A release assay in vitro showed that up to 91.36% of the total plasmid DNA could be sustainably released from the pFDNA-LDH@SiO 2 -NPs within 288 hours. The LDH@SiO 2 nanoparticles had very low toxicity. Additionally, their high transfection efficiency in vitro was detected by fluorescent microscopy. Intranasal immunization of specific pathogen-free chickens with pFDNA-LDH@SiO 2 -NPs induced stronger cellular, humoral, and mucosal immune responses and achieved a greater sustained release effect than intramuscular naked plasmid DNA, and the protective efficacy after challenge with the strain F 48 E 9 with highly virulent (mean death time of chicken embryos ≤60 hours, intracerebral pathogenicity index in 1 -day-old chickens >1.6) was 100%. Based on the results, LDH@SiO 2 nanoparticles can be used as a delivery carrier for mucosal immunity of Newcastle disease DNA vaccine, and have great application potential in the future.

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Formulating Poly(Lactide-Co-Glycolide) Particles for Plasmid DNA Delivery

Cited by 70 publications

References 99 publications

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

Newcastle disease virus vaccine encapsulated in biodegradable nanoparticles for mucosal delivery of a human vaccine

Synthesis, characterization, and immune efficacy of layered double hydroxide@SiO2 nanoparticles with shell-core structure as a delivery carrier for Newcastle disease virus DNA vaccine

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