FORMULASI DAN UJI EFEKTIVITAS ANTIOKSIDAN SEDIAAN KRIM EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L. ) DENGAN MENGGUNAKAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

Abstract

Belimbing wuluh leaves (Averrhoa bilimbi L.) Contains flavonoids, phenols, alkaloids, tannins and coumarin. The aim of this research is formulated ethanol extract of Belimbing Wuluh leaves in the form of cream preparations, as well as test the effectiveness of antioxidants using the DPPH method. Extract was made by maceration method using ethanol 96% formulated into a cream with a concentration of 3%, 5%, 7%. The results of the cream preparations evaluation before and after cycling test meet the requirements of organoleptis, homogeneity, pH, adhesiveness, coverage. The test results of antioxidant activity with the DPPH method using the most effective UV-Vis spectrophotometer as the antioxidant cream of the leaves extract Belimbing Wuluh is 7% has a value of IC50 = 0.118 ppm and vitamin C as comparator has a value of IC50 = 0.0327 ppm. From this research can be concluded that Belimbing Wuluh leaves ethanol extract cream can be formulated as a stable cream and has a very strong antioxidant activity. Keywords: : Belimbing Wuluh Leaves,cream, antioxidant,DPPH ABSTRAK Daun Belimbing Wuluh (Averrhoa bilimbi L.) mengandung senyawa flavonoid, fenol, alkaloid, tanin dan kumarin. Tujuan penelitian ini membuat formulasi ekstrak etanol daun Belimbing Wuluh dalam bentuk sediaan krim, serta menguji efektivitas antioksidan menggunakan metode DPPH. Pembuatan ekstrak dilakukan dengan metode maserasi menggunakan etanol 96 % diformulasi menjadi krim dengan konsentrasi 3%, 5% ,7%. Hasil evaluasi sediaan krim sebelum dan sesudah cycling test memenuhi persyaratan organoleptis, homogenitas, pH, daya lekat, daya sebar. Hasil uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometer UV-Vis yang paling efektif sebagai krim antioksidan dari ekstrak daun Belimbing Wuluh adalah 7 % memiliki nilai IC50 = 0,118 ppm dan vitamin C sebagai pembanding memiliki nilai IC50 = 0,0327 ppm. Dari penelitian ini dapat disimpulkan bahwa krim ekstrak etanol daun Belimbing Wuluh dapat diformulasikan sebagai sediaan krim yang stabil dan memiliki aktivitas antioksidan yang sangat kuat. Kata kunci : Daun Belimbing Wuluh, Krim, Antioksidan, DPPH

Antioxidant serum gel formulation with a combination of secretome from mesenchymal stem cells and rosemary oil

“…Incubate in a dark room for 30 minutes at room temperature. Take absorbance readings at the maximum wavelength from the measurements that have been made [14] .…”

“…These results indicate that all antioxidant gel pH values range within the normal skin pH range. A pH value that is too acidic can irritate the skin, while a pH that is too alkaline can cause dry and flaky skin [14] .…”

“…Where x is the sample concentration, and y is the % inhibition. The IC50 value is the concentration of antioxidant compounds, which causes a 50% decrease from the initial DPPH concentration [14,15].…”

“…Viscosity is a test that determines the value of the resistance of a liquid to flow. The higher the value, the higher the level of viscosity of the substance; the more significant the preparation's resistance to flow, affecting the preparation's dispersion and adhesion [14]. The viscosity can also affect the mobility of the active ingredients in the base.…”

Pengaruh Waktu Ekstraksi dengan Metode Microwave Assisted Extraction (MAE) terhadap Aktivitas Antioksidan Ekstrak Daun Belimbing Wuluh (Averrhoa Bilimbi L.)

“…Aziz et al, (2014) menyatakan ekstrak daun belimbing wuluh dapat berpotensi sangat besar sebagai antibakteri dan antikanker. Ariem et al, (2020) melaporkan daun belimbing wuluh mempunyai nilai IC50 lebih rendah dari buah belimbing wuluh secara berturut-turut sebesar 11,86 ppm dan 44,01 ppm. Wijayakusuma, (2006) melaporkan senyawa bioaktif yang didapati pada daun belimbing wuluh meliputi fenol, flavonoid, tanin dan sulfur.…”