Formulae and Program to Determine Total Carotenoids and Chlorophylls A and B of Leaf Extracts in Different Solvents

Wellburn, A. R.; Lichtenthaler, Hartmut K.

doi:10.1007/978-94-017-6368-4_3

Cited by 337 publications

(209 citation statements)

References 2 publications

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“…Total percentage tissue nitrogen was determined using replicates of 0Á2 g of dry ground P. alpinum tissue and combusted via elemental analyser (Truspec CN, LECO Corporation, USA); nitrogen content is expressed as a percentage of tissue. To determine carotenoid pigments, 100 mg of tissue was homogenized in acetone and then diluted (ten times) in acetone, and the pigment content was determined spectrophotometrically as previously described (Wellburn and Lichtenthaler, 1984).…”

Section: Physiology Of P Alpinummentioning

confidence: 99%

Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,Polytrichastrum alpinum

Shortlidge

Eppley

Köhler

et al. 2016

Ann Bot

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Background and Aims The Western Antarctic Peninsula is one of the most rapidly warming regions on Earth, and many biotic communities inhabiting this dynamic region are responding to these well-documented climatic shifts. Yet some of the most prevalent organisms of terrestrial Antarctica, the mosses, and their responses to warming have been relatively overlooked and understudied. In this research, the impacts of 6 years of passive warming were investigated using open top chambers (OTCs), on moss communities of Fildes Peninsula, King George Island, Antarctica.Methods The effects of experimental passive warming on the morphology, sexual reproductive effort and stress physiology of a common dioicous Antarctic moss, Polytrichastrum alpinum, were tested, gaining the first speciesspecific mechanistic insight into moss responses to warming in the Antarctic. Additionally community analyses were conducted examining the impact of warming on overall moss percentage cover and sporophyte production in intact Antarctic moss communities.Key Results Our results show a generally greater percentage moss cover under warming conditions as well as increased gametangia production in P. alpinum. Distinct morphological and physiological shifts in P. alpinum were found under passive warming compared with those without warming: warmed mosses reduced investment in cellular stress defences, but invested more towards primary productivity and gametangia development.Conclusions Taken together, results from this study of mosses under passive warming imply that in ice-free moss-dominated regions, continued climate warming will probably have profound impacts on moss biology and colonization along the Western Antarctic Peninsula. Such findings highlight the fundamental role that mosses will play in influencing the terrestrialization of a warming Antarctica.

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Section: Physiology Of P Alpinummentioning

confidence: 99%

Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,Polytrichastrum alpinum

Shortlidge

Eppley

Köhler

et al. 2016

Ann Bot

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“…In the case of 80% acetone extraction, different coefficients should be used, as suggested in more recent works (Wellburn and Lichtentaler, 1983;Porra et al, 1989). The latter paper contains correction factors for values obtained by the Arnon method.…”

Section: I1mentioning

confidence: 99%

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

Bassi

Rigoni

Giacometti

1990

Photochem & Photobiology

158

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“…Chlorophyll and carotenoid were determined in 80% acetone, using the equations of Wellburn and Lichtenthaler (1984). Absorption spectra of chlorophyll a/b proteins were taken in 10 mM Hepes pH 7.6,0.06% dodecyl P-D-maltoside using a Perkin Elmer lambda 5 spectrophotometer.…”

Section: Other Methodsmentioning

confidence: 99%

Heterogenous lipid distribution among chlorophyll‐binding proteins of photosystem II in maize mesophyll chloroplasts

Trémolières¹,

Dainese

Bassi

1994

European Journal of Biochemistry

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Photosystem I1 membrane fractions from dark-adapted mesophyll chloroplasts of maize were solubilized in different concentrations of dodecyl P-D-maltoside. Chlorophyll-binding proteins from photosystem I1 were isolated either by ultracentrifugation on a sucrose gradient, or by flat bed isoelectric focusing and identified by gel electrophoresis analysis for their polypeptide composition. Lipid and fatty acid compositions were determined in complexes prepared by both methods and also in purified light-harvesting complex 11, in minor chlorophyll alb binding complexes 29, 26, 24, in photosystem I1 antennae (chlorophyll-protein complexes 43, 47) and in the photosystem I1 reaction centers chlorophyll-protein complexes. Comparative analysis of the results suggests that a true heterogeneity exists in the lipid class distribution among the different chlorophyll-protein complexes in this region of the photosynthetic membrane. Photosystem I1 core fractions prepared either by ultra-centrifugation on a sucrose gradient or by isoelectric focusing were found significantly enriched in monogalactosyldiacylglycerol ; fractionation of the photosystem I1 core in its components showed that it was the chlorophyll-protein complexes 43 and 47 which were mainly responsible for this enrichment. One of them, the chlorophyll-protein complex 47, was found containing monogalactos yldiacylglycerol and having a very high level of saturated fatty acids. The minor chlorophyll alb binding linkers (chlorophyll-protein complexes 24, 26 and 29) retain a largely higher amount of lipids than all other complexes and especially of highly unsaturated galactolipids.Concerning the main light-harvesting antenna (LHCII), it is demonstrated that phosphatidylglycerol is strongly linked to the complex if it cannot be detached at high detergent concentration, while many galactolipids (which nevertheless represent the major lipid classes) are lost. This main light-harvesting complex has been fractionated into several families by isoelectric focusing showing a marked difference in lipid and polypeptide composition. A spectacular increase in the phosphatidylglycerol content was observed in the fraction migrating near the anode and enriched in a 26-kDa polypeptide; but this result is difficult to interpret in physiological terms as it was shown that phosphatidylglycerol alone, because of its negative charge, also migrates toward the anode in isoelectric focusing.In photosynthesis, the two major functions of light-harvesting and electron transport occur in a thylakoid which is the predominant membrane in the plant leaf cell representing up to 80% of the total cellular membranes (Forde and Steer, 1976). Thylakoids are organized into two major functional domains. The appressed or granal membranes and the nonappressed or stromal membranes contain different complements of electron transport and binding complexes participating in the photosynthetic process. The lipid composition of the thylakoid is unique since it always contains four lipids never found in other cellular m...

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Formulae and Program to Determine Total Carotenoids and Chlorophylls A and B of Leaf Extracts in Different Solvents

Cited by 337 publications

References 2 publications

Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,Polytrichastrum alpinum

Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,Polytrichastrum alpinum

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

Heterogenous lipid distribution among chlorophyll‐binding proteins of photosystem II in maize mesophyll chloroplasts

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