Formoterol and budesonide inhibit rhinovirus infection and cytokine production in primary cultures of human tracheal epithelial cells

Yamaya, Mutsuo; Nishimura, Hidekazu; Nadine, Lusamba; Kubo, Hiroshi; Nagatomi, Ryoichi

doi:10.1016/j.resinv.2014.03.004

Cited by 30 publications

(65 citation statements)

References 49 publications

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“…2001a; Yamaya et al. 2014b). Briefly, for the HTE and HNE cell cultures , surface epithelial cells of the human tracheal and nasal mucosa were isolated by exposure to 0.05% protease and cultured in plastic tubes with round bottoms (16 mm in diameter and 125 mm in length; Becton Dickinson, Franklin Lakes, NJ) or on coverslips in Petri dishes coated with human placental collagen as previously described (Suzuki et al.…”

Section: Methodsmentioning

confidence: 99%

“…2001a; Yamaya et al. 2014b). Virus‐containing supernatants were added to the replicate HEF cells in the wells (200 μ L/well) of the 96‐well plates.…”

Section: Methodsmentioning

confidence: 99%

“…2001a; Yamaya et al. 2014b). The rates of RV14 release into the supernatants are expressed as the tissue culture infective dose (TCID 50 ) units/mL (Suzuki et al.…”

Section: Methodsmentioning

confidence: 99%

“…Human embryonic fibroblast (HEF) cells (HFL‐III cells, Riken Bio Resource Center Cell Bank, Cell No: RCB0523; Tsukuba, Japan) were cultured as previously described (Yamaya et al. 2014b).…”

Section: Methodsmentioning

confidence: 99%

“…The RV14 (a major group RV) stock was prepared from a patient with a common cold by infecting HEF cells as previously described (Yamaya et al. 2014b).…”

Section: Methodsmentioning

confidence: 99%

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The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells

et al. 2015

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The anti-inflammatory effects of macrolides may be associated with a reduced frequency of exacerbation of chronic obstructive pulmonary disease (COPD). However, because the long-term use of antibiotics may promote the growth of drug-resistant bacteria, the development of a treatment to prevent COPD exacerbation with macrolides that do not exert anti-bacterial effects is necessary. Additionally, the inhibitory effects of nonantibiotic macrolides on the replication of rhinovirus (RV), which is the major cause of COPD exacerbation, have not been demonstrated. Primary cultures of human tracheal epithelial cells and nasal epithelial cells were pretreated with the nonantibiotic macrolide EM900 for 72 h prior to infection with a major group RV type 14 rhinovirus (RV14) and were further treated with EM900 after infection. Treatment with EM900 before and after infection reduced RV14 titers in the supernatants and viral RNA within the cells. Moreover, cytokine levels, including interleukin (IL)-1β and IL-6, were reduced in the supernatants following RV14 infection. Treatment with EM900 before and after infection also reduced the mRNA and protein expression of intercellular adhesion molecule-1 (ICAM-1), which is the receptor for RV14, after infection and reduced the activation of the nuclear factor kappa-B protein p50 in nuclear extracts after infection. Pretreatment with EM900 reduced the number and fluorescence intensity of the acidic endosomes through which RV RNA enters the cytoplasm. Thus, pretreatment with EM900 may inhibit RV infection by reducing the ICAM-1 levels and acidic endosomes and thus modulate the airway inflammation associated with RV infections.

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Section: Methodsmentioning

confidence: 99%

“…2001a; Yamaya et al. 2014b). Virus‐containing supernatants were added to the replicate HEF cells in the wells (200 μ L/well) of the 96‐well plates.…”

Section: Methodsmentioning

confidence: 99%

“…2001a; Yamaya et al. 2014b). The rates of RV14 release into the supernatants are expressed as the tissue culture infective dose (TCID 50 ) units/mL (Suzuki et al.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The RV14 (a major group RV) stock was prepared from a patient with a common cold by infecting HEF cells as previously described (Yamaya et al. 2014b).…”

Section: Methodsmentioning

confidence: 99%

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The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells

et al. 2015

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Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM‐1 levels and endosomal acidification and is inhibited by L‐carbocisteine

Yamaya¹,

Nomura²,

Arakawa³

et al. 2016

Immunity Inflam & Disease

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Increased viral replication and cytokine production may be associated with the pathogenesis of asthma attacks in rhinovirus (RV) infections. However, the association between increased RV replication and enhanced expression of intercellular adhesion molecule‐1 (ICAM‐1), a receptor for a major RV group, in airway epithelial cells has remained unclear. Furthermore, the inhibitory effects of mucolytics, which have clinical benefits in asthmatic subjects, are uncertain. Human nasal epithelial (HNE) cells were infected with type 14 rhinovirus (RV14), a major RV group. RV14 titers and cytokine concentrations, including interleukin (IL)‐6 and IL‐8, in supernatants, RV14 RNA replication and susceptibility to RV14 infection were higher in HNE cells obtained from subjects in the allergic group (allergic subjects) than in those from subjects in the non‐allergic group (non‐allergic subjects). ICAM‐1 expression and the number and fluorescence intensity of acidic endosomes from which RV14 RNA enters the cytoplasm were higher in HNE cells from allergic subjects, though substantial amounts of interferon (IFN)‐γ and IFN‐λ were not detected in the supernatant. The abundance of p50 and p65 subunits of transcription factor nuclear factor kappa B (NF‐κB) in nuclear extracts of the cells from allergic subjects was higher compared to non‐allergic subjects, and an inhibitor of NF‐κB, caffeic acid phenethyl ester, reduced the fluorescence intensity of acidic endosomes as well as RV titers and RNA. Furthermore, a mucolytic agent, L‐carbocisteine, reduced RV14 titers and RNA levels, cytokine release, ICAM‐1 expression, the fluorescence intensity of acidic endosomes, and NF‐κB activation. The increased RV14 replication observed in HNE cells from allergic subjects might be partly associated with enhanced ICAM‐1 expression and decreased endosomal pH through NF‐κB activation. L‐Carbocisteine inhibits RV14 infection by reducing ICAM‐1 and acidic endosomes and may, therefore, modulate airway inflammation caused by RV infection in allergic subjects.

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Allergie und der respiratorische Infekt

Stockert¹

2020

Allergieprävention

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Formoterol and budesonide inhibit rhinovirus infection and cytokine production in primary cultures of human tracheal epithelial cells

Cited by 30 publications

References 49 publications

The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells

The non-antibiotic macrolide EM900 inhibits rhinovirus infection and cytokine production in human airway epithelial cells

Increased rhinovirus replication in nasal mucosa cells in allergic subjects is associated with increased ICAM‐1 levels and endosomal acidification and is inhibited by L‐carbocisteine

Allergie und der respiratorische Infekt

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