Formation of Tubulovesicular Carriers from Endosomes and Their Fusion to the trans-Golgi Network

Hierro, Aitor; Gershlick, David C.; Rojas, Adriana L.; Bonifacino, Juan S.

doi:10.1016/bs.ircmb.2015.05.005

Cited by 15 publications

(12 citation statements)

References 275 publications

(306 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Silencing of any of the GARP subunits inhibits the retrograde transport of MPRs, TGN46 and the B subunit of Shiga toxin (Perez-Victoria et al, 2008). More recent evidence also suggests the involvement of additional tethering complexes in the endosome-to-Golgi transport pathway, such as the COG and Dsl1 complexes (Arasaki et al, 2013;Hierro et al, 2015;Laufman et al, 2011).…”

Section: Tethering At the Tgnmentioning

confidence: 99%

Bidirectional traffic between the Golgi and the endosomes – machineries and regulation

Progida

Bakke

2016

Journal of Cell Science

View full text Add to dashboard Cite

The bidirectional transport between the Golgi complex and the endocytic pathway has to be finely regulated in order to ensure the proper delivery of newly synthetized lysosomal enzymes and the return of sorting receptors from degradative compartments. The high complexity of these routes has led to experimental difficulties in properly dissecting and separating the different pathways. As a consequence, several models have been proposed during the past decades. However, recent advances in our understanding of endosomal dynamics have helped to unify these different views. We provide here an overview of the current insights into the transport routes between Golgi and endosomes in mammalian cells. The focus of the Commentary is on the key molecules involved in the trafficking pathways between these intracellular compartments, such as Rab proteins and sorting receptors, and their regulation. A proper understanding of the bidirectional traffic between the Golgi complex and the endolysosomal system is of uttermost importance, as several studies have demonstrated that mutations in the factors involved in these transport pathways result in various pathologies, in particular lysosome-associated diseases and diverse neurological disorders, such as Alzheimer's and Parkinson's disease.

show abstract

Section: Tethering At the Tgnmentioning

confidence: 99%

Bidirectional traffic between the Golgi and the endosomes – machineries and regulation

Progida

Bakke

2016

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…Whereas other intercompartmental transport steps involve small, coated vesicles, endosomal retrieval is largely mediated by tubular-vesicular carriers that bud from EEs and fuse with either recycling endosomes (REs) or the TGN ( Gallon and Cullen, 2015 ; Hierro et al ., 2015 ). Both budding and fusion of the carriers depend on complex molecular machineries that are recruited from the cytosol onto membranes.…”

Section: Introductionmentioning

confidence: 99%

“…Both budding and fusion of the carriers depend on complex molecular machineries that are recruited from the cytosol onto membranes. A core component of the endosomal budding machinery is the retromer complex, which comprises a VPS26-VPS29-VPS35 heterotrimer and various combinations of sorting nexin (SNX) proteins, including a SNX1/2-SNX5/6 heterodimer, SNX3, and/or SNX27 ( Gallon and Cullen, 2015 ; Hierro et al ., 2015 ). Various other components, such as membrane phosphatidylinositol 3-phosphate (PtdIns3P; Burda et al ., 2002 ), the small GTPase Rab7 ( Rojas et al ., 2008 ; Seaman et al ., 2009 ), the Rab-GAP TBC1D5 ( Seaman et al ., 2009 ), the WASH complex ( Gomez and Billadeau, 2009 ), and dynactin ( Hong et al ., 2009 ; Wassmer et al ., 2009 ), interact with retromer to mediate different steps in the process of tubule formation and translocation.…”

Section: Introductionmentioning

confidence: 99%

TSSC1 is novel component of the endosomal retrieval machinery

Gershlick

Schindler

Chen

et al. 2016

MBoC

Self Cite

View full text Add to dashboard Cite

show abstract

“…Retrograde trafficking can be initiated at different levels of the endosome (early, late, and recycling endosomes) in a process that is termed retrograde sorting (10,11). Exit of cargo molecules is mediated by different retrograde-sorting proteins for delivery to the TGN and Golgi apparatus, where a diverse set of tethering factors act on acceptor membranes (12,13).…”

mentioning

confidence: 99%

MicroRNA 199a-5p Attenuates Retrograde Transport and Protects against Toxin-Induced Inhibition of Protein Biosynthesis

Aranda

Rathjen

Johannes

et al. 2018

Molecular and Cellular Biology

View full text Add to dashboard Cite

Retrograde transport (RT) allows cells the retrieval of receptors and other cellular cargoes to the Golgi contributing to the maintenance of cellular homeostasis. This transport route is also commonly used by several bacterial toxins to exert their deleterious actions on eukaryotic cells. While the retrograde transport process has been well characterized, the contribution of microRNAs (miRNAs) in regulating this cellular transport mechanism remains unknown. Here, we identified that the intronic miRNA family, , coordinate genes regulating RT and endosome trafficking. We demonstrate that miR-199a-5p attenuates the expression of Vps26A, Rab9B and M6PR, thereby controlling RT from endosomes to Trans Golgi network (TGN). Importantly, we found that overexpression of Vps26A construct resistant to miR-199a-5p inhibitory action abrogates the effect of miR-199a-5p on RT. Finally, we demonstrate that miR-199-5p overexpression attenuates Shiga toxin (STx-1)-mediated inhibition of protein biosynthesis. In summary, our work identifies the first non-coding RNA that influences RT and reduces protein biosynthesis inhibition caused by bacterial toxins.

show abstract

Formation of Tubulovesicular Carriers from Endosomes and Their Fusion to the trans-Golgi Network

Cited by 15 publications

References 275 publications

Bidirectional traffic between the Golgi and the endosomes – machineries and regulation

Bidirectional traffic between the Golgi and the endosomes – machineries and regulation

TSSC1 is novel component of the endosomal retrieval machinery

MicroRNA 199a-5p Attenuates Retrograde Transport and Protects against Toxin-Induced Inhibition of Protein Biosynthesis

Contact Info

Product

Resources

About