Formation of the Ca2+-activated photoprotein obelin from apo-obelin and mRNA inside human neutrophils

Campbell, Anthony K.; Patel, Anish; Razavi, Zia; McCapra, Frank

doi:10.1042/bj2520143

Cited by 24 publications

(5 citation statements)

References 30 publications

(34 reference statements)

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“…These pioneering studies provided the basis for the design and development, only few years later, of novel methods for incorporating the obelin apoprotein and mRNA isolated from the hydroid, Obelia, into the cytoplasm of intact human neutrophils. This approach avoided the consumption of Ca 2+ ‐activated photoproteins during cell activation or injury and provided a means to monitor protein synthesis in living cells .…”

Section: Application Of Light‐emitting Proteins To Bioanalysismentioning

confidence: 99%

Bioluminescence imaging: a shining future for cardiac regeneration

Roura

Gálvez‐Montón

Bayés‐Genís

2013

J Cellular Molecular Medi

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Advances in bioanalytical techniques have become crucial for both basic research and medical practice. One example, bioluminescence imaging (BLI), is based on the application of natural reactants with light-emitting capabilities (photoproteins and luciferases) isolated from a widespread group of organisms. The main challenges in cardiac regeneration remain unresolved, but a vast number of studies have harnessed BLI with the discovery of aequorin and green fluorescent proteins. First described in the luminous hydromedusan Aequorea victoria in the early 1960s, bioluminescent proteins have greatly contributed to the design and initiation of ongoing cell-based clinical trials on cardiovascular diseases. In conjunction with advances in reporter gene technology, BLI provides valuable information about the location and functional status of regenerative cells implanted into numerous animal models of disease. The purpose of this review was to present the great potential of BLI, among other existing imaging modalities, to refine effectiveness and underlying mechanisms of cardiac cell therapy. We recount the first discovery of natural primary compounds with light-emitting capabilities, and follow their applications to bioanalysis. We also illustrate insights and perspectives on BLI to illuminate current efforts in cardiac regeneration, where the future is bright.

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Section: Application Of Light‐emitting Proteins To Bioanalysismentioning

confidence: 99%

Bioluminescence imaging: a shining future for cardiac regeneration

Roura

Gálvez‐Montón

Bayés‐Genís

2013

J Cellular Molecular Medi

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“…Essential to this story has been the measurement of free Ca 2+ in live cells. The photoproteins aequorin and obelin, and then fluors such as fura2, have revolutionized cell biology by enabling free Ca 2+ signals to be measured and imaged in living cells, whole organs, and even whole organisms (Campbell et al . 1988; Sala‐Newby et al .…”

Section: Molecular Biodiversity In Actionmentioning

confidence: 99%

Save those molecules! Molecular biodiversity and life*

Campbell

2003

Journal of Applied Ecology

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Summary1. Biodiversity involves diversity of species, genetics and habitats. But there is a fourth source of biodiversity -molecular biodiversity -without which evolution cannot occur, either in the origin of a new species, its survival and development, or its eventual extinction. 2 . Molecular biodiversity is distinct from genetic diversity, though both ultimately depend on inheritable DNA. It occurs within one individual, between individuals of the same species, between related species, within and between phyla and ecosystems, and throughout evolution. There is also a crucial evolutionary role for 'hidden' molecular biodiversity in 'bad' genes. These highlight what Darwin and Wallace missed, the origin of a biological process, or a species. 3. Genetic engineering of bioluminescence, coupled with molecular imaging, has given us a wonderful technology for lighting up the molecular biodiversity of individual living cells, and even whole organisms. This has highlighted a major challenge -when is a biological process analogue or digital? 4. Synthesis and applications . The care of our planet, and ecology in the 21st century, depend on a new thinking based on molecular biodiversity. My go-home message is that ecologists must grasp the opportunities presented by advances in molecular and cellular biology. But, although molecules are at the centre of modern biology and medicine, science begins and ends with a curiosity about the whole -the cell, the organ or the individual organism, within a particular ecosystem.

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“…The coelenterazine concentration was determined by the extinction coefficient in methanol, 8900 cm Ϫ1 M Ϫ1 at 434 nm (14). The activation mixture was incubated for 16 h at 4°C in the dark.…”

Section: Activation Of Apoobelinmentioning

confidence: 99%