Formation of Tethered Bilayer Lipid Membranes on Gold Surfaces:  QCM-Z and AFM Study

Dorvel, Brian; Keizer, Henk M.; Fine, Daniel H.; Vuorinen, Jorma; Dodabalapur, A.; Duran, Randolph S.

doi:10.1021/la0610396

Cited by 43 publications

(36 citation statements)

References 51 publications

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“…The results obtained in this study are quite different to those published by Dorvel et al 32 They showed a biphasic kinetic curve for the adsorption and reorganization of the DPhyTL into a self-assembled monolayer. The QCM monitoring of DPhyTL deposition at 300 M ͑ϳ1 ϫ 10 −4 mg/ ml͒ on gold showed a linear frequency shift, which reached Ϫ15 Hz after 5 h, followed by a stronger frequency decrease from 8 to 21 h until a plateau at a frequency shift of ⌬f ϳ −50 Hz was reached.…”

Section: Monolayer Assemblycontrasting

confidence: 99%

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Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

et al. 2009

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Tethered bilayer lipid membranes are promising biomimetic architectures. Their formation has been investigated using four different surface sensitive techniques, including optical, acoustic, and electrical methods. The lipid bilayers are built in a two-step procedure; the proximal layer is formed by self-assembly and is then completed to a bilayer by fusion with small vesicles. The different technical approaches revealed specific aspects of the layer formation processes, namely, first a fast adsorption process followed by a longer rearrangement period. Similar phenomena have been observed for the vesicle fusion process. The results allow for a more controlled assembly protocol for the preparation of highly insulating lipid membranes.

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Section: Monolayer Assemblycontrasting

confidence: 99%

“…Dorvel et al reported on the fusion of anchorlipid vesicles in the formation of the monolayer, which is not very probable, since the lipids are completely soluble in ethanol. 32 Furthermore, the combination of electrical and optical techniques offers more insight into the processes.…”

Section: Introductionmentioning

confidence: 99%

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

et al. 2009

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“…Several sensing platforms that are capable of hosting transmembrane proteins within BLM based devices have been proposed, including: patch clamp arrays (Mathes, 2006;Dale et al, 2007;Lau et al, 2006;Wee-Liat Ong et al, 2007;Li et al, 2006;Fertig et al, 2003), patterns of tethered BLMs (Taylor et al, 2007;Han et al, Orth et al, 2003;Andersson et al, 2007;Dorvel et al, 2007;Pera and Fritz, 2007), BLMs formed by deposition of giant unilamellar vesicles over micro-and nano-pores (Mach et al, 2008;Schimdt et al, 2000) and arrays of BLMs, both suspended and hydrogelsupported (Maurer et al, 2007;Castellana and Cremer, 2006;Le Pioufle et al, 2008;Suzuki et al, 2004;Wilk et al, 2007;Baaken et al, 2008). Each of these platforms offer both advantages and disadvantages: for instance, tethered bilayers allow for geometrical patterning of analysis sites, similar to protein printing technology, but the membrane fluidity, and consequently the conformational structure of any reconstituted ion channels, can be altered (Deverall et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Microfluidic array platform for simultaneous lipid bilayer membrane formation

Zagnoni

Sandison

Morgan

2009

Biosensors and Bioelectronics

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A Self‐assembly Route for Double Bilayer Lipid Membrane Formation

Han¹,

Achalkumar²,

Cheetham³

et al. 2010

ChemPhysChem

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Solid supported lipid membranes provide a simple biomimetic model system that is suitable for studying a wide range of membrane related phenomena and importantly permits the use of a number of surface analytical techniques [1][2][3][4][5][6][7][8] from AFM to impedance spectroscopy to be used in characterising such processes. Additionally, it is also believed that such systems could find application for drug screening, biosensing or in protein separation/crystallisation. [9] In nature there are however a number of situations in which double bilayers naturally occur, for example in mitochondria [10] or complexes which span two lipid bilayers at gap junctions, [11] and for this reason it would be desirable to be able to create double bilayer mimics as an extension of the supported bilayer field. It has been previously demonstrated that one can create such structures using the Langmuir-Blodgett technique, [12] however, this has the drawback that it is not compatible with the incorporation of transmembrane proteins and the film is created by transfer through the air-water interface. Hence approaches based on self-assembly from vesicles would provide a significant advance in the type of applications that double bilayers could be used for. Murray et al. have recently demonstrated, a previously observed phenomenon, that a second bilayer can be assembled on top of a streptavidin protein film, attached to a first bilayer.[13] Further, Chung et al. have also shown that giant unilamellar vesicle (GUV) rupture can lead to the formation of a second bilayer tethered to a first bilayer using complementary DNA sequences.[14] Similarly, GUV rupture onto a lipid bilayer to form model intermembrane junctions has been reported by Kaizuka and Groves; [15] and Tabaei et al. have demonstrated a method whereby DNA duplexes were used to tether multiple diskshaped lipid "bicelles" to a bilayer.[16] While these systems could not be used for studying double bilayer phenomena directly, they demonstrate that the principle of achieving double bilayers via self-assembly is feasible. Here we describe a new method to form double bilayer lipid membranes (dBLMs) on solid supports using NHS/EDC [17] chemistry [hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid sodium salt (NHS) and N-Ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) Figure 1]. In this approach, two biomembranes, one of which contains the amine-functionalised cholesterol derivative 1 and the other which contains the CO 2 H-funtionalised cholesterol derivative 2 are covalently joined together by an NHS/EDC mediated reaction [18,19] as shown schematically in Figure 1. Details of the synthesis and characterization of the cholesterol derivatives 1 and 2 are given in the Supporting Information. These dBLMs can be formed both on functionalized Au and on silicon oxide surfaces. Here we describe their formation on a silicon oxide surfaces, monitored using a combination of fluorescence microscopy and atomic force spectroscopy.Vesicles containing Egg PC, reagent 2, DOTAP, and the re...

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Formation of Tethered Bilayer Lipid Membranes on Gold Surfaces: QCM-Z and AFM Study

Cited by 43 publications

References 51 publications

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Microfluidic array platform for simultaneous lipid bilayer membrane formation

A Self‐assembly Route for Double Bilayer Lipid Membrane Formation

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