Formation of Teeth

Reichenmiller, Katharina; Klein, Christian

doi:10.1002/9783527619443.ch55

Cited by 3 publications

(3 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Inside the spheres, mineral particles could be clearly distinguished from the surrounding extracellular matrix by means of backscattered electron contrast (“material contrast”) [ 18 ]. The morphology as well as the detected vesicular constrictions of mineral particles from the cells in the present study is comparable with the origin and form of cellular-induced minerals regarding the odontoblastic mineralization front in vivo [ 42 ]. Previous findings regarding mineral formation related with odontoblasts were mostly visualized using histological, immunohistochemical and light microscopic analysis [ 43 ].…”

Section: Discussionsupporting

confidence: 81%

Odontoblast-like differentiation and mineral formation of pulpsphere derived cells on human root canal dentin in vitro

et al. 2017

View full text Add to dashboard Cite

BackgroundThe revitalization or regeneration of the dental pulp is a preferable goal in current endodontic research. In this study, human dental pulp cell (DPC) spheres were applied to human root canal samples to evaluate their potential adoption for physiological tissue-like regeneration of the dental root canal by odontoblastic differentiation as well as cell-induced mineral formation.MethodsDPC were cultivated into three-dimensional cell spheres and seeded on human root canal specimens. The evaluation of sphere formation, tissue-like behavior and differentiation as well as mineral formation of the cells was carried out with the aid of optical light microscopy, immunohistochemical staining and scanning electron microscopy (SEM).ResultsSpheres and cells migrated out of the spheres showed an intense cell-cell- and cell-dentin-contact with the formation of extra cellular matrix. In addition, the ingrowth of cell processes into dentinal tubules and the interaction of cell processes with the tubule walls were detected by SEM-imaging. Immunohistochemical staining of the odontoblast specific matrix proteins, dentin matrix protein-1, and dentin sialoprotein revealed an odontoblast-like cell differentiation in contact with the dentin surface. This differentiation was confirmed by SEM-imaging of cells with an odontoblast specific phenotype and cell induced mineral formation.ConclusionsThe results of the present study reveal the high potential of pulp cells organized in spheres for dental tissue engineering. The odontoblast-like differentiation and the cell induced mineral formation display the possibility of a complete or partial “dentinal filling” of the root canal and the opportunity to combine this method with other current strategies.

show abstract

Section: Discussionsupporting

confidence: 81%

Odontoblast-like differentiation and mineral formation of pulpsphere derived cells on human root canal dentin in vitro

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Taj po jed no sta vlje ni pri stup mo že se pri me ni ti i na na sta ja nje zu ba, iako je si tu a ci ja mno go slo že ni ja jer se zu bi ne sa sto je od jed nog, već od tri mi ne ra li zo va na tki va (den tin, gleđ, ce ment) i jed nog ne mi ne ra li zo va nog (zub na pul pa). Iako su struk tu ra i sa stav, a ti me i funk ci ja gle đi (95% neo r gan ske kom po nente, 4% vo de i 1% or gan ske ma tri ce), den ti na (70% neo r ganske kom po nen te, 10% vo de i 20% or gan ske ma tri ce) i ce men ta (61% neo r gan ske kom po nen te, 12% vo de i 27% or gan ske matri ce ) raz li či ti, pro ce si nji ho vog na stan ka slič ni su i uklju ču ju osnov ne pro ce se ta lo že nja -nu kle a ci ju, rast kri sta la i zre nje [1].…”

Section: Fizičko-hemijska Svojstva Kalcijumovih Fosfataunclassified

“…This simplified approach can be applied to the formation of teeth, although the situation is more complex because teeth do not consist of one but of three mineralized (dentin, enamel, cement) and one non-mineralized tissue (dental pulp). Although structure, composition and function of enamel (95% inorganic components, 4% water and 1% of the organic matrix), dentin (70% inorganic components, 10% water and 20% organic matrix) and cement (61% inorganic components 12% water and 27% organic matrix) is different, the process of their formation is similar and include basic processes of deposition -nucleation, crystal growth and maturation [1].…”

Section: Introductionmentioning

confidence: 99%

Physico-chemical properties of calcium phosphates

Babić-Ivančić

Sikirić

2012

SERBIAN DENT J

View full text Add to dashboard Cite

Calcium phosphates have important role in biological and pathological mineralization. While only one of calcium phosphates, carbonate apatite, represents the main mineral component of teeth and bones, octacalcium phosphate, calcium hydrogenphosphate dihydrate and beta-tricalcium phosphate occur in pathological deposits. From the stand-point of chemists, processes of biological and pathological mineralization could be considered as deposition of inorganic phase within organic matrix, i.e. formation of inorganic-organic composites. Although this approach is very simplified at first glance, it allows clarification of important issues related to biomineralization (e.g. what is the role of individual components of organic matrix in the emerging solid tissue), and design and preparation of new materials for hard tissue regeneration (e.g. process of transformation after implantation). The importance of investigation about calcium phosphates will be presented through the overview of basic physico-chemical reactions related to the formation and transformation of biologically relevant calcium phosphates and their interaction with various organic additives in the laboratory

show abstract

Synthesis of Collagen‐Like Sequential Polypeptides Containing O‐Phospho‐L‐Hydroxyproline and Preparation of Electrospun Composite Fibers for Possible Dental Application

Ohkawa

Hayashi

Kameyama

et al. 2008

Macromolecular Bioscience

View full text Add to dashboard Cite

A synthetic route is described for collagen-like polypeptides constructed from O-phospho-L-hydroxyproline [Hyp(PO(3)H(2))] residues. Using the synthetic polypeptides and a natural protein, gelatin, fine fibers and their network structures (ESNWs) were prepared via electrospinning. The composite ESNWs can induce the mineralization of calcium phosphate. The phosphoryl groups of the Hyp(PO(3)H(2)) residues affect both the crystalline phase and amount of the calcium phosphate, depending on the chemical structure in the repeating sequence. The composite ESNWs can be developed as a biocompatible replacement of the extracellular matrix of hard tissues, and thus can be applied as dental materials for restoration of dental cavities or as a sealant for pits and fissures.

show abstract

Formation of Teeth

Cited by 3 publications

References 59 publications

Odontoblast-like differentiation and mineral formation of pulpsphere derived cells on human root canal dentin in vitro

Odontoblast-like differentiation and mineral formation of pulpsphere derived cells on human root canal dentin in vitro

Physico-chemical properties of calcium phosphates

Synthesis of Collagen‐Like Sequential Polypeptides Containing O‐Phospho‐L‐Hydroxyproline and Preparation of Electrospun Composite Fibers for Possible Dental Application

Contact Info

Product

Resources

About