Formation of taste aversion and preference in protein synthesis inhibition in rats

Serova, O. N.; Solov'ea, N. A.; Lagutina, L. V.; Obukhova, M. F.

doi:10.1007/bf02359410

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…Our previous studies demonstrated that acute microinfusion of BDNF in anesthetized adult rats 24 h before CTA training enhances the retention of this task (Castillo et al, 2006). Moreover, it has been observed that protein translation inhibition impairs CTA consolidation but not acquisition (Rosenblum et al, 1993; Serova et al, 1996; Berman and Dudai, 2001; Moguel-Gonzalez et al, 2008) and we recently reported that acute intracortical delivery of BDNF reverses the deficit in CTA memory, caused by inhibition of IC protein synthesis due to anisomycin administration (Moguel-Gonzalez et al, 2008). These evidences suggest that BDNF is an essential protein synthesis product for the establishment of the CTA–LTM.…”

Section: Introductionmentioning

confidence: 86%

Late protein synthesis-dependent phases in CTA long-term memory: BDNF requirement

Martínez-Moreno

Rodríguez-Durán

Escobar

2011

Front. Behav. Neurosci.

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It has been proposed that long-term memory (LTM) persistence requires a late protein synthesis-dependent phase, even many hours after memory acquisition. Brain-derived neurotrophic factor (BDNF) is an essential protein synthesis product that has emerged as one of the most potent molecular mediators for long-term synaptic plasticity. Studies in the rat hippocampus have been shown that BDNF is capable to rescue the late-phase of long-term potentiation as well as the hippocampus-related LTM when protein synthesis was inhibited. Our previous studies on the insular cortex (IC), a region of the temporal cortex implicated in the acquisition and storage of conditioned taste aversion (CTA), have demonstrated that intracortical delivery of BDNF reverses the deficit in CTA memory caused by the inhibition of IC protein synthesis due to anisomycin administration during early acquisition. In this work, we first analyze whether CTA memory storage is protein synthesis-dependent in different time windows. We observed that CTA memory become sensible to protein synthesis inhibition 5 and 7 h after acquisition. Then, we explore the effect of BDNF delivery (2 μg/2 μl per side) in the IC during those late protein synthesis-dependent phases. Our results show that BDNF reverses the CTA memory deficit produced by protein synthesis inhibition in both phases. These findings support the notion that recurrent rounds of consolidation-like events take place in the neocortex for maintenance of CTA memory trace and that BDNF is an essential component of these processes.

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Section: Introductionmentioning

confidence: 86%

Late protein synthesis-dependent phases in CTA long-term memory: BDNF requirement

Martínez-Moreno

Rodríguez-Durán

Escobar

2011

Front. Behav. Neurosci.

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“…Control injections consisted of sterile saline administered via the same routes and volumes as the treatment injections. Prior reports using the same dose and route of CXM administration have suggested that the PSI, although sufficient to disrupt the acquisition of CTA, is inhibited by only 43% in the rat brain (Serova et al, 1996).…”

Section: Methodsmentioning

confidence: 99%

“…The conditioned response (CR) resulting from this pairing of flavor with illness is manifested by the animal's subsequent avoidance of the CS. Of interest to the present study are those findings indicating that CTA is dependent on de novo protein synthesis in the brain (Berman & Dudai, 2001; Serova, Solov'ea, Lagutina, & Obukhova, 1996; Tucker & Gibbs, 1976, 1979; Tucker & Oei, 1982). CTA studies have demonstrated that PSI infusions into the gustatory cortex impair acquisition (Rosenblum, Meiri, & Dudai, 1993); infusions into the central nucleus of the amygdala impair consolidation (Bahar, Dorfman, & Dudai, 2004); and infusions into the basolateral nucleus (Bahar et al, 2004), insular cortex (Berman & Dudai, 2001), or the ventromedial prefrontal cortex (Akirav et al, 2006) disrupt extinction.…”

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confidence: 94%

Cycloheximide impairs reconsolidation of a contextually reactivated memory in a conditioned taste aversion paradigm.

Flint¹,

Marino²

2007

Behavioral Neuroscience

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Rats were used to examine the impact of systemic protein synthesis inhibition (PSI) on the reconsolidation of a contextually reactivated memory of conditioned taste aversion (CTA). Rats were administered intraperitoneal injections of saline or lithium chloride (LiCl; .15 M) following exposure to a novel sucrose solution in a unique context. Seven days later, rats were injected subcutaneously with saline or cycloheximide (CXM; 1 mg/kg) and returned to their home cage or placed into the CTA training context in the absence of the target conditioned stimulus to reactivate the training memory. At testing, LiCl-trained rats that had been given CXM at reactivation had significantly greater difference scores (sucrose-water) in comparison with LiCl/CXM rats that had not been given a reactivation treatment and LiCl/saline memory-reactivated rats. These results suggest that context re-exposure effectively reactivates memory of CTA training that may be weakened through PSI. Extinction tests revealed rapid attenuation of taste aversions in all of the LiCl-injected groups. The involvement of taste-potentiated aversions and the role of the context in taste aversion conditioning are discussed.

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“…There is evidence that long-term CTA memory requires protein synthesis. Administration of general protein synthesis inhibitors into the lateral ventricles (Tucker and Gibbs 1976;Serova et al 1995) or cerebral cortex (Rosenblum et al 1993) attenuates long-term expression of CTAs. Long-term CTA has been attenuated also by inhibiting the synthesis of specific proteins with antisense oligonucleotides: c-Fos or CREB within the amygdala (Lamprecht and Dudai 1996;Lamprecht et al 1997), and c-Fos in the brain stem after fourth-ventricular administration (Swank et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Rapid, Labile, and Protein Synthesis– Independent Short-Term Memory in Conditioned Taste Aversion

Houpt

Berlin²

1999

Learn. Mem.

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Short-term memory is a rapid, labile, and protein-synthesis-independent phase of memory. The existence of short-term memory in conditioned taste aversion (CTA) learning has not been demonstrated formally. To determine the earliest time at which a CTA is expressed, we measured intraoral intake of sucrose at 15 min, 1 hr, 6 hr, or 48 h after contingent pairing of an intraoral infusion of 5% sucrose (6.6 ml over 6 min) and toxic lithium chloride injection (76 mg/kg). Rats were implanted with intraoral catheters to allow presentation of taste solutions at arbitrary times. Intraoral intake was measured under conditions of long-delay, single-trial learning typical of CTA. Rats decreased intraoral intake of sucrose at 15 min after contingent pairing of sucrose and LiCl, but not after noncontingent LiCl or sucrose. Thus CTA learning can be expressed rapidly. To determine if short-term CTA memory is labile and decays in the absence of long-term memory, we measured intraoral intake of sucrose after pairing sucrose with low doses of LiCl. Rats received an intraoral infusion of 5% sucrose (6 ml/6 min); 30 min later LiCl was injected at three different doses (19, 38, or 76 mg/kg). A second intraoral infusion of sucrose was administered 15 min, 1 hr, 3 hr, 4.5 hr, 6 hr, or 48 hr later. The formation of long-term CTA memory was dependent on the dose of LiCl paired with sucrose during acquisition. Low doses of LiCl induced a CTA that decayed within 6 hr after pairing. Central administration of the protein synthesis inhibitor cycloheximide prior to LiCl injection blocked long-term CTA expression at 6 and 48 hr, but not short-term CTA expression at 1 hr. Thus, short-term memory for CTA learning exists that is acquired rapidly and independent of protein synthesis, but labile in the absence of long-term memory formation.

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Formation of taste aversion and preference in protein synthesis inhibition in rats

Cited by 10 publications

References 3 publications

Late protein synthesis-dependent phases in CTA long-term memory: BDNF requirement

Late protein synthesis-dependent phases in CTA long-term memory: BDNF requirement

Cycloheximide impairs reconsolidation of a contextually reactivated memory in a conditioned taste aversion paradigm.

Rapid, Labile, and Protein Synthesis– Independent Short-Term Memory in Conditioned Taste Aversion

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