Formation of silver nanoparticles by human gut microbiota

Yin, Naiyi; Gao, Rui; Knowles, Brett M; Wang, Jiasheng; Wang, Pengfei; Sun, Guoxin; Cui, Yanshan

doi:10.1016/j.scitotenv.2018.09.312

Cited by 22 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…coli bacteria observed by TEM and show that cells contain additional membranous structures [42]; in Figure 8(b), we observe these additional structures. Yin et al have described using SEM AgNPs located on the cytoplasmic membrane and cell wall [70], similar results we observed in Figure 8(b).…”

supporting

confidence: 90%

Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

Alvarez-Cirerol

López-Torres

Rodríguez-León

et al. 2019

Journal of Nanomaterials

View full text Add to dashboard Cite

Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Syndrome (AHPNS) is a disease produced by gram-negative bacteria Vibrio parahaemolyticus (V. parahaemolyticus), which has caused declines in worldwide production of a white shrimp Litopenaeus vannamei (L. vannamei). In this work, we propose the implementation of silver nanoparticles (AgNPs) synthesized with Rumex hymenosepalus (Rh) extract as an alternative on V. parahaemolyticus control. AgNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED). AgNP mean sizes by DLS were 80.82±1.16 nm and sizes between 2 and 10 nm by TEM, with a zeta potential of −47.72±1.05 mV. This study evaluated AgNPs and Rh antimicrobial capacity on V. parahaemolyticus at different concentrations; the minimum inhibitory concentration (MIC) found was 25 μg/mL for AgNPs and 220 μg/mL for Rh. Additionally, were carried out time-kill curves and reactive oxygen species (ROS) generation for 1 and 4 MIC. Both concentrations (MIC) were tested for toxicity on Artemia nauplii from Artemia franciscana (A. franciscana), because nauplii were used as biocarriers for AgNPs and Rh extract on L. vannamei. Once the shrimp were treated, they were challenged with Vibrio infection and it was found that those who were treated with both agents showed greater survival than the control. V. parahaemolyticus and postlarval samples were taken from the bioassay and fixed and prepared for TEM and SEM in order to search NPs in internal structure of bacteria and the hepatopancreatic area of shrimps; AgNPs were detected in both cases. AgNPs and Rh extract show antibacterial properties on the infected shrimp with V. parahaemolyticus. The action mechanisms are interaction with the bacterial membrane and ROS generation; these effects are produced by both agents.

show abstract

supporting

confidence: 90%

Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

Alvarez-Cirerol

López-Torres

Rodríguez-León

et al. 2019

Journal of Nanomaterials

View full text Add to dashboard Cite

show abstract

“…As a result, those lactobacilli were highly susceptible to AgNMs while the opportunistic pathogens were not. [115] These studies have shown their antibacterial effects on gut bacteria; however, it is also reported that the Ag + could be reduced into Ag NMs because of the metal reductase produced by gut microbiota, [116] suggesting that gut microbiota can also act on nanomaterials and determine their bioactivity in living organism.…”

Section: Effects Of Nanomaterials On Gut Microbiotamentioning

confidence: 99%

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

Cui

Bao

Wang

et al. 2020

Small

View full text Add to dashboard Cite

The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201907665. Engineered nanomaterials (ENMs) are used in food additives, food packages, and therapeutic purposes owing to their useful properties, Therefore, human beings are orally exposed to exogenous nanomaterials frequently, which means the intestine is one of the primary targets of nanomaterials. Consequently, it is of great importance to understand the interaction between nanomaterials and the intestine. When nanomaterials enter into gut lumen, they inevitably interact with various components and thereby display different effects on the intestine based on their locations; these are known as location-oriented effects (LOE). The intestinal LOE confer a new biological-effect profile for nanomaterials, which is dependent on the involvement of the following biological processes: nanomucus interaction, nano-intestinal epithelial cells (IECs) interaction, nanoimmune interaction, and nano-microbiota interaction. A deep understanding ofNM-induced LOE will facilitate the design of safer NMs and the development of more efficient nanomedicine for intestine-related diseases. Herein, recent progress in this field is reviewed in order to better understand the LOE of nanomaterials. The distant effects of nanomaterials coupling with microbiota are also highlighted. Investigation of the interaction of nanomaterials with the intestine will stimulate other new research areas beyond intestinal nanotoxicity. www.advancedsciencenews.com

show abstract

“…fluorescens strain accumulates copper ions in quantities up to 500 mg/g of dry biomass [9], and the strain of P. mendocina sorbs copper in quantities up to 558 mg/g of dry biomass and cadmium to 0.75 mg/g of dry biomass [10]. P. pseudoalcaligenes reasonably well accumulates lead and copper ions, their number reach 271.7 mg/g and 46.8 mg/g of dry biomass, respectively [11,12].…”

Section: Resultsmentioning

confidence: 99%

A study on heavy metal sorption properties of intestinal microbiota in vitro

Sizentsov

Кван

et al. 2019

E3S Web Conf.

View full text Add to dashboard Cite

Water and soil pollution by heavy metals has led to their accumulation in the ground and waters, to a sharp decline of ecosystem biopotential, and to foodstuff contamination, resulting in internal pollutions in bodies of humans and animals with food, air, water. In this regard, the purpose of our study was to examine the impact of heavy metals on the growth of representatives of rats’ intestinal microbiota and to estimate sorption characteristics of these microorganisms. The research was conducted in three stages: i) a study of essential and xenobiotic elements’ biotoxicity; ii) their impact on the growth of the microbiota in periodic cultivation; and iii) studying bioaccumulative characteristics. In the research, we discovered that all investigated organisms had pronounced resistance to lead and iron, which was confirmed by the results of the evaluating the impact on the growth and the accumulating characteristics of the studied microorganisms (more than 50%). The strain of E. faecium has the most pronounced sorption characteristics – its level of accumulation amounted for iron –63.3%, lead – 61%, zinc – 38.4%, copper – 11.4%, and cadmium – 33.2%, minimum values were obtained in samples of L. acidophilus, which sorbed these elements in concentrations of 46.2%, 42.3%, 10.3%, 3.8%, and 5.3%, respectively.

show abstract

Formation of silver nanoparticles by human gut microbiota

Cited by 22 publications

References 34 publications

Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

A study on heavy metal sorption properties of intestinal microbiota in vitro

Contact Info

Product

Resources

About