Formation of Pseudomonas aeruginosa PAO1 biofilms in the presence of hydrogen peroxide. The effect of the aiiA gene

Plyuta, V. A.; Andreenko, J. V.; Kuznetsov, Alexander; Khmel, I. A.

doi:10.3103/s089141681304006x

Cited by 11 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional evidence for importance of QS system in bacterial biofilm formation is provided by the work, in which another stress factor (hydrogen peroxide) was shown to stimulate growth of P. aerugi nosa PAO1 biofilms. Moreover, this effect (similar to our experiments with azithromycin) was not observed in the presence of the plasmid pME6860, which con tains the heterologous gene aiiA encoding N acyl homoserine lactonase [24]. Finally, antibiotics may affect the regulatory systems involving cyclic digua nosine monophosphate (c di GMP), which partici pates in the biosynthesis of components of the polysaccaride biofilm matrix, as was shown for ami noglycosides in case of E. coli [25].…”

Section: Resultssupporting

confidence: 70%

Regulation of biofilm formation by Pseudomonas chlororaphis in an in vitro system

et al. 2015

Self Cite

View full text Add to dashboard Cite

The mutants of Pseudomonas chlororaphis 449 with completely or partially suppressed accumula tion of N acyl homoserine lactones exhibited the absence or a pronounced decrease of their capacity for stim ulation of biofilm growth in the presence of azithromycin. Biofilms of the wild type strain preformed in the presence of the stimulatory azithromycin concentrations exhibited more intense staining with a polysaccha ride specific dye 1,9 dimethyl methylene blue (DMMB) and were more resistant to heat shock. These find ings indicate accumulation of the structural matrix polysaccharides, which play a protective role under con ditions of thermal shock. Extremely low azithromycin concentrations (0.001-0.01 µg/mL) inhibit biofilm formation by P. chlororaphis 449 and P. chlororaphis 66 with suppression of the synthesis of DMMB staining polysaccharides.

show abstract

Section: Resultssupporting

confidence: 70%

Regulation of biofilm formation by Pseudomonas chlororaphis in an in vitro system

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Results and Discussionsupporting

confidence: 61%

“…The theoretical maximum concentration of HP electrochemically generated along the electrode surface was calculated and is equal to 18 μM (Section SI-13). The experimental results obtained from the external dosage of HP on biofilm formation at the OCP condition (Section SI-12) as well as previous studies 67,68 suggest that this concentration of ∼20 μM HP is still subinhibitory for both planktonic and sessile communities of P. aeruginosa PAO1. Electrochemically generated HP may have little detrimental effect on planktonic bacterial cells only at the very early stage of biofilm formation immediately after deposition of bacteria on the electrode surface.…”

Section: Environmental Science and Technologysupporting

confidence: 62%

“…In agreement with previous studies, the application of external HP had a negligible influence on biofilm formation or cell viability (Section SI-12). − Only at concentrations an order of magnitude higher than those observed in the batch mode experiments (20 μM) did we observe a small difference between biovolume and cell viability. For these experiments, the increased EPS production at the elevated dosage of HP (analyzed with a CLSM, Section SI-12) also suggests a physiological response of the biofilm to negate the harmful effect of HP. , A thorough discussion on HP generation in the flow cell is provided in Section SI-13.…”

Section: Results and Discussionmentioning

confidence: 72%

See 1 more Smart Citation

Influence of Electric Fields on Biofouling of Carbonaceous Electrodes

Pandit

Shanbhag²,

Mauter³

et al. 2017

Environ. Sci. Technol.

View full text Add to dashboard Cite

Biofouling commonly occurs on carbonaceous capacitive deionization electrodes in the process of treating natural waters. Although previous work reported the effect of electric fields on bacterial mortality for a variety of medical and engineered applications, the effect of electrode surface properties and the magnitude and polarity of applied electric fields on biofilm development has not been comprehensively investigated. This paper studies the formation of a Pseudomonas aeruginosa biofilm on a Papyex graphite (PA) and a carbon aerogel (CA) in the presence and the absence of an electric field. The experiments were conducted using a two-electrode flow cell with a voltage window of ±0.9 V. The CA was less susceptible to biofilm formation compared to the PA due to its lower surface roughness, lower hydrophobicity, and significant antimicrobial properties. For both positive and negative applied potentials, we observed an inverse relationship between biofilm formation and the magnitude of the applied potential. The effect is particularly strong for the CA electrodes and may be a result of cumulative effects between material toxicity and the stress experienced by cells at high applied potentials. Under the applied potentials for both electrodes, high production of endogenous reactive oxygen species (ROS) was indicative of bacterial stress. For both electrodes, the elevated specific ROS activity was lowest for the open circuit potential condition, elevated when cathodically and anodically polarized, and highest for the ±0.9 V cases. These high applied potentials are believed to affect the redox potential across the cell membrane and disrupt redox homeostasis, thereby inhibiting bacterial growth.

show abstract

“…The citrate-capped AgNPs having 10 nm average size display synergistic effect with aztreonam against the P. aeruginosa biofilms.The effect is due to an improved permeation of the antibiotic into biofilm matrix (Plyuta et al 2013;Habash et al 2014). Additionally, AgNPs are also known to generate reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH.…”

Section: Antimicrobial Activity Of Agnpsmentioning

confidence: 99%

Silver nanoparticles as antimicrobial therapeutics: current perspectives and future challenges

2018

View full text Add to dashboard Cite

Utility of silver metal in antimicrobial therapy is an accepted practice since ages that faded with time because of the identification of a few silver resistant strains in the contemporary era. A successive development of antibiotics soon followed. However, due to an indiscriminate and unregulated use coupled with poor legal control measures and a dearth of expertise in handling the critical episodes, the antibiotics era has already seen a steep decline in the past decades due to the evolution of multi-drug resistant 'superbugs' which pose a sizeable challenge to manage with. Due to limited options in the pipeline and no clear strategy in the forefront, the aspirations for novel, MDR focused drug discovery to target the 'superbugs' arose which once again led to the rise of AgNPs in antimicrobial research. In this review, we have focused on the green routes for the synthesis of AgNPs, the mode of microbial inhibition by AgNPs, synergistic effect of AgNPs with antibiotics and future challenges for the development of nano-silver-based therapeutics.

show abstract

Formation of Pseudomonas aeruginosa PAO1 biofilms in the presence of hydrogen peroxide. The effect of the aiiA gene

Cited by 11 publications

References 35 publications

Regulation of biofilm formation by Pseudomonas chlororaphis in an in vitro system

Regulation of biofilm formation by Pseudomonas chlororaphis in an in vitro system

Influence of Electric Fields on Biofouling of Carbonaceous Electrodes

Silver nanoparticles as antimicrobial therapeutics: current perspectives and future challenges

Contact Info

Product

Resources

About