Formation of porous PLGA scaffolds by a combining method of thermally induced phase separation and porogen leaching

Yang, Yanfang; Zhao, Jin; Zhao, Yunhui; Wen, Liang; Yuan, Xiaoyan; Fan, Yubo

doi:10.1002/app.28147

Cited by 75 publications

(50 citation statements)

References 32 publications

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“…The results indicate that the pore size of both PLGA (Figure 1(a)) and PLGA/β-TCP scaffolds (Figure 1(b)) was mainly distributed in the range of 100-350 μm. The porosity of the two scaffolds was approximately 90%, as determined by modified ethanol replacement method [5]. The SEM micrograph of the PLGA/β-TCP scaffold (Figure 1(d)) suggests that there is an even distribution of β-TCP on the pore wall of the scaffold.…”

Section: Plga and Plga/β-tcp Scaffolds Before And After Degradationmentioning

confidence: 92%

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Effect of degradation of PLGA and PLGA/β-TCP scaffolds on the growth of osteoblasts

et al. 2010

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Osteoblasts were cultured on porous scaffolds of poly(L-lactide-co-glycolide) (PLGA) and PLGA/β-tricalcium phosphate (β-TCP) to evaluate their cytocompatibility. The proliferation of the cells on both scaffolds was examined before and after in vitro degradation for 4, 8 and 12 weeks under static (shaking water bath) and dynamic (cyclic loading) conditions. Results indicate that porous PLGA and PLGA/β-TCP scaffolds have good biocompatibility and can be used as effective templates for guiding the growth of osteoblasts. The degradation of the scaffolds affects the proliferation of osteoblasts and the cell viability decreased with the degradation time.

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Section: Plga and Plga/β-tcp Scaffolds Before And After Degradationmentioning

confidence: 92%

“…(ii) Preparation of porous scaffolds. Porous PLGA and PLGA/β-TCP scaffolds were fabricated using a technique combining thermally-induced phase separation and porogen leaching [5]. Briefly, PLGA was dissolved in chloroform to form a homogeneous 0.15 g/mL solution and blended with sucrose particles (85 vol%).…”

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confidence: 99%

Effect of degradation of PLGA and PLGA/β-TCP scaffolds on the growth of osteoblasts

et al. 2010

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“…26 PLGA 85:15 (M w = 50,000-75,000 g/ mol) (Sigma-Aldrich) was dissolved in dichloromethane (Sigma-Aldrich). Sieved salt crystals (Fisher Scientific) (300-500 mm) were added to the PLGA solution and the PLGA/ salt paste was pressed into a mold and incubated for 6 h to allow the dichloromethane to evaporate.…”

Section: Preparation Of 3d Scaffoldsmentioning

confidence: 99%

Imaging of Poly(α-hydroxy-ester) Scaffolds with X-ray Phase-Contrast Microcomputed Tomography

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Somo

et al. 2012

Tissue Engineering Part C: Methods

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Porous scaffolds based on poly(a-hydroxy-esters) are under investigation in many tissue engineering applications. A biological response to these materials is driven, in part, by their three-dimensional (3D) structure. The ability to evaluate quantitatively the material structure in tissue-engineering applications is important for the continued development of these polymer-based approaches. X-ray imaging techniques based on phase contrast (PC) have shown a tremendous promise for a number of biomedical applications owing to their ability to provide a contrast based on alternative X-ray properties (refraction and scatter) in addition to X-ray absorption. In this research, poly(a-hydroxy-ester) scaffolds were synthesized and imaged by X-ray PC microcomputed tomography. The 3D images depicting the X-ray attenuation and phase-shifting properties were reconstructed from the measurement data. The scaffold structure could be imaged by X-ray PC in both cell culture conditions and within the tissue. The 3D images allowed for quantification of scaffold properties and automatic segmentation of scaffolds from the surrounding hard and soft tissues. These results provide evidence of the significant potential of techniques based on X-ray PC for imaging polymer scaffolds. A typical approach involves combinations of biomaterial scaffolds, soluble factors, and cells to promote vascularized tissue formation. Polymeric scaffolds often play a critical role in the success of a tissue engineering therapy, as they provide structural support and mechanical strength, and can directly influence tissue response based on physical, mechanical, and chemical signaling. The physical structure of these scaffolds is critical to their success, and quantitative three-dimensional (3D) imaging tools are needed that enable a more thorough analysis of tissue engineering scaffolds.Poly(a-hydroxy esters), including poly(lactic-co-glycolic acid) (PLGA) and poly(L-lactic acid) (PLLA), are some of the most widely investigated biomaterials and have been studied in a broad range of tissue engineering applications.2-5 A variety of techniques are available for generating scaffolds with an interconnected porous structure and tunable degradation kinetics. Success in a particular tissue engineering application requires choosing the appropriate polymer, porous structure, mechanical properties, and the degradation rate of these materials. Degradation of PLGA and PLLA in vitro has been extensively studied in an attempt to predict behavior in the body.6-13 However, tracking 3D tissue invasion and material degradation in vitro and in vivo without sample alteration is very important for assessing the efficacy of the tissue engineering strategy. Current methods employed in tissue engineering are invasive, require sample destruction, and provide only 2D images. Recently, a multiagency government working group (MultiAgency Tissue Engineering Science Interagency Working Group) identified imaging technologies for a 3D analysis of polymeric scaffolds and engineered tissues as a...

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“…Porosity of the scaffold was determined by a modified liquid replacement method [19,20]. The scaffold was put into a glass bottle which was connected with the vacuum system.…”

Section: Scaffold Characterizationmentioning

confidence: 99%

Fabrication of poly(lactic acid) scaffolds by a modified solvent casting/porogen leaching method

et al. 2010

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Abstract:A modified solvent casting/particulate leaching (SC/PL) method was developed to fabricate thick three-dimensional poly(lactic acid) (PLA) scaffolds with controlled pore size and porosity, and much shorter processing time. The pasty mixture of PLA/CHCl 3 /NaCl was sealed in a Buchner funnel by a piston with a suction flask connected. A vacuum pump was used to facilitate the volatilization of solvent. The scaffolds were obtained by subsequent removal of NaCl in deionized water. The pore size within the scaffold was in good accordance with the particle size of NaCl (< 210, 210~310, 310~420 and 420~500 μm), all of which had more than 89 % of porosity, 1.95 ± 0.2 MPa of compressive modulus and non-cytotoxic to L929 cells. To demonstrate the applicability of the SC/VV/PL method, thick cylindrical and tubular porous scaffolds have been produced in this study. Compared with the traditional SC/PL without vacuum volatilization, the modified method, namely, solvent casting/vacuum volatilization/particulate leaching (SC/VV/PL) method, provide a more efficient way to fabricate thick scaffolds.

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Formation of porous PLGA scaffolds by a combining method of thermally induced phase separation and porogen leaching

Cited by 75 publications

References 32 publications

Effect of degradation of PLGA and PLGA/β-TCP scaffolds on the growth of osteoblasts

Effect of degradation of PLGA and PLGA/β-TCP scaffolds on the growth of osteoblasts

Imaging of Poly(α-hydroxy-ester) Scaffolds with X-ray Phase-Contrast Microcomputed Tomography

Fabrication of poly(lactic acid) scaffolds by a modified solvent casting/porogen leaching method

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