Formation of polar metabolites from aldrin by pea and bean root preparations

McKinney, James D.; Mehendale, Harihara M.

doi:10.1021/jf60190a012

Cited by 10 publications

(1 citation statement)

References 6 publications

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“…Aldrin and dieldrin was analyzed by a gas-chromatograph fitted with an electron capture detector as previously described (17). Protein concentrations were determined using the biuret method or the method of Lowry et al (13).…”

Section: Extraction and Analysesmentioning

confidence: 99%

Aldrin Epoxidase Activity in Developing Rabbit Lung

Mehendale

1980

Pediatr Res

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SummaryPulmonary aldrin epoxidase activity was determined in 2-, 4, 6 , &, I t , and ldweek-old male rabbits using isolated perfused lung preparations and in vim incubation techniques. In the perfusion experiments, it was determined that aldrin epoxidase activity increased with age up to 12 weeks of age. Although there was an increase in the weight of lungs with age, this did not appear to be related to the increase in aldrin epoxidation. In vim experiments carried out using the same substrate to tissue ratio failed to exhibit an age-related increase in the aldrin epoxidase activity. Extrapolated values for aldrin epoxidation from in vibw experiments yielded much higher values than realized from perfusion experiments. Accessibility of the substrate to the enzymatic sites, saturation of the accessible e v e system, and limiting availability of the cofactor necessary for the oxidative reaction remain as the possible mechanisms for the discrepancy between perfusion and in vim incubation studies. SpeculationPulmonary aldrin epoxidase activity determined using isolated perfused lung preparations does not correlate with in vim results obtained from incubations fortified with cofactors. However, the ratio of the two may become a useful predictive factor to obtain realistic contribution of an organ to the metabolism of xenobiotics.

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Section: Extraction and Analysesmentioning

confidence: 99%

Aldrin Epoxidase Activity in Developing Rabbit Lung

Mehendale

1980

Pediatr Res

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Environmental applications of mass spectrometry

Alford

1975

Biol. Mass Spectrom.

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Publications from 1969 through 1974 reporting organic and spark source mass spectrometric studies on environmental samples have been compiled. Emphasis is placed on pollutants identified, but some methods and techniques are discussed. The 396 reports cited vary from those reporting hundreds of identified pollutants to those describing the analysis of only one sample containing one or a few pollutants. Reports concerning forensic, synthetic and standard samples, and analyses for drug residues. metabolites and degradation products are not included

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