Formation of Persistent Hyperplastic Primary Vitreous in Ephrin-A5<sup>−/−</sup>Mice

Son, Alexander I.; Sheleg, Michal; Cooper, Margaret A.; Sun, Yunjuan; Kleiman, Norman J.; Zhou, Ran

doi:10.1167/iovs.13-12706

Cited by 20 publications

(15 citation statements)

References 83 publications

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“…However, since previously, we have reported that ephrin-A5 −/− mice developed ocular abnormalities [55, 56], we wanted to confirm that the lack of aggression is not due to the inability of the mice to see the intruder. Indeed, ephrin-A5 −/− mice, despite the vision impairments, investigated the intruder when introduced into their home cage, suggesting that they were able to sense the presence of another animal in their cage.…”

Section: Discussionmentioning

confidence: 99%

Ephrin-A5 regulates inter-male aggression in mice

Sheleg

Yochum

Richardson

et al. 2015

Behavioural Brain Research

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The Eph family of receptor tyrosine kinases play key roles in both the patterning of the developing nervous system and neural plasticity in the mature brain. To determine functions of ephrin-A5, a GPI-linked ligand to the Eph receptors, in animal behavior regulations, we examined effects of its inactivation on male mouse aggression. When tested in the resident-intruder paradigm for offensive aggression, ephrin-A5-mutant animals (ephrin-A5−/−) exhibited severe reduction in conspecific aggression compared to wild-type controls. On the contrary, defensive aggression in the form of target biting was higher in ephrin-A5−/− mice, indicating that the mutant mice are capable of attacking behavior. In addition, given the critical role of olfaction in aggressive behavior, we examined the ability of the ephrin-A5−/− mice to smell and found no differences between the mutant and control animals. Testosterone levels in the mutant mice were also found to be within the normal range. Taken together, our data reveal a new role of ephrin-A5 in the regulation of aggressive behavior in mice.

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Section: Discussionmentioning

confidence: 99%

Ephrin-A5 regulates inter-male aggression in mice

Sheleg

Yochum

Richardson

et al. 2015

Behavioural Brain Research

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“…PFV has been reported in other mammalian species, particularly in dogs [9–11] and in genetic mouse models [12–20]. Studies with these animals have helped immensely with our current understanding of the disease.…”

Section: Persistent Fetal Vasculature (Pfv)mentioning

confidence: 99%

βA3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye

Zigler

Valapala

Shang

et al. 2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background Persistent fetal vasculature (PFV) is a human disease in which the fetal vasculature of the eye fails to regress normally. The fetal, or hyaloid, vasculature nourishes the lens and retina during ocular development, subsequently regressing after formation of the retinal vessels. PFV causes serious congenital pathologies and is responsible for as much as 5% of blindness in the United States. Scope of review The causes of PFV are poorly understood, however there are a number of animal models in which aspects of the disease are present. One such model results from mutation or elimination of the gene (Cryba1) encoding βA3/A1-crystallin. In this review we focus on the possible mechanisms whereby loss of functional βA3/A1-crystallin might lead to PFV. Major conclusions Cryba1 is abundantly expressed in the lens, but is also expressed in certain other ocular cells, including astrocytes. In animal models lacking βA3/A1-crystallin, astrocyte numbers are increased and they migrate abnormally from the retina to ensheath the persistent hyaloid artery. Evidence is presented that the absence of functional βA3/A1-crystallin causes failure of the normal acidification of endolysosomal compartments in the astrocytes, leading to impairment of certain critical signaling pathways, including mTOR and Notch/STAT3. General significance The findings suggest that impaired endolysosomal signaling in ocular astrocytes can cause PFV disease, by adversely affecting the vascular remodeling processes essential to ocular development, including regression of the fetal vasculature. This article is part of a Special Issue entitled Crystallin Biochemistry in Health and Disease.

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“…Two issues in the commercialization of AO retinal imaging techniques are cost and the space required for such instruments. Efforts have been made to make AO-assisted instruments such as the AOSLO [ 22 – 24 ], the AO-OCT [ 22 , 23 , 25 ] and the AO fundus camera [ 25 ] more compact and therefore easier to adopt in the clinic. Such efforts indicate that the development of more compact systems is beneficial for the wider use of AO retinal imaging technologies.…”

Section: Introductionmentioning

confidence: 99%

Compact, modular and in-plane AOSLO for high-resolution retinal imaging

Young¹,

Morris²,

Saunter³

et al. 2018

Biomed. Opt. Express

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The adaptive optics scanning laser ophthalmoscope (AOSLO) was first developed in 2002 and since then the technology has been adopted in several laboratories around the world, for both clinical and psychophysical research. There have been a few major design implementations of the AOSLO. The first used on-axis tilted spherical mirrors in a planar arrangement, and the second minimized the build up of astigmatism present in the first design by using a non-planar arrangement. Other designs have avoided astigmatism by using custom-made toroidal mirrors or by using lenses on-axis, rather than mirrors.We present a new design implementation for an AOSLO that maintains a planar optical alignment without the build up astigmatism using compact, reconfigurable modules based on an Offner relay system. We additionally use an off-the-shelf digital oscilloscope for data capture and custom-written Python code for generating and analyzing the retinal images. This design results in a compact system that is simple to align and, being composed of modular relays, has the potential for additional components to be added. We show that this system maintains diffraction-limited image quality across the field of view and that cones are clearly resolved in the central retina. The modular relay design is generally applicable to any system requiring one or more components in the pupil conjugate plane. This is likely to be useful for any point-scanned system, such as a standard scanning laser ophthalmoscope or non-ophthalmic confocal imaging system.

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Formation of Persistent Hyperplastic Primary Vitreous in Ephrin-A5^−/−Mice

Abstract: Ephrin-A5 is a critical factor that regulates primary vitreous regression.

Cited by 20 publications

References 83 publications

Ephrin-A5 regulates inter-male aggression in mice

Ephrin-A5 regulates inter-male aggression in mice

βA3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye

Compact, modular and in-plane AOSLO for high-resolution retinal imaging

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Formation of Persistent Hyperplastic Primary Vitreous in Ephrin-A5−/−Mice

Abstract: Ephrin-A5 is a critical factor that regulates primary vitreous regression.

Cited by 20 publications

References 83 publications

Ephrin-A5 regulates inter-male aggression in mice

Ephrin-A5 regulates inter-male aggression in mice

βA3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye

Compact, modular and in-plane AOSLO for high-resolution retinal imaging

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Product

Resources

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Formation of Persistent Hyperplastic Primary Vitreous in Ephrin-A5^−/−Mice