Formation of Nanoemulsions by Low‐Energy Methods and Their Use as Templates for the Preparation of Polymeric Nanoparticles

Calderó, Gabriela; Solans, Conxita

doi:10.1002/9783527628698.hgc090

Cited by 1 publication

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“…Nano-emulsions were prepared by the phase inversion composition (PIC) method (Patil and Panyam, 2009;Calderó and Solans, 2012). 4 g of nano-emulsions were formed by stepwise addition of 0.16 M electrolyte solution, (a phosphate buffered saline, PBS solution, which components are indicated in Section 2.1) to mixtures of surfactant and oil at 25 ºC.…”

Section: Nano-emulsion Preparationmentioning

confidence: 99%

“…(Patil and Panyam, 2009). Nano-emulsion templating represents a versatile and simple technology for the fabrication of nanoparticles for different biomedical uses (Calderó et al, 2011;Landfester, 2001; Calderó and Solans, 2012) by which the above mentioned disadvantages can be avoided. Nano-emulsions are emulsions with droplet sizes typically between 20 and 200 nm Solans and Solè, 2012) that can be controlled by appropriate selection of system components, composition and process parameters.…”

Section: Introductionmentioning

confidence: 99%

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Novel non-viral gene delivery systems composed of carbosilane dendron functionalized nanoparticles prepared from nano-emulsions as non-viral carriers for antisense oligonucleotides

Fornaguera

Grijalvo

Galán

et al. 2015

International Journal of Pharmaceutics

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The development of novel and efficient delivery systems is often the limiting step in fields such as antisense therapies. In this context, poly(d,l-lactide-co-glycolide) acid (PLGA) nanoparticles have been obtained by a versatile and simple technology based on nano-emulsion templating and low-energy emulsification methods, performed in mild conditions, providing good size control. O/W polymeric nano-emulsions were prepared by the phase inversion composition method at 25°C using the aqueous solution/polysorbate80/[4 wt% PLGA in ethyl acetate] system. Nano-emulsions formed at oil-to-surfactant (O/S) ratios between 10/90-90/10 and aqueous contents above 70 wt%. Nano-emulsion with 90 wt% of aqueous solution and O/S ratio of 70/30 was chosen for further studies, since they showed the appropriate characteristics to be used as nanoparticle template: hydrodynamic radii lower than 50 nm and enough kinetic stability. Nanoparticles, prepared from nano-emulsions by solvent evaporation, showed spherical shape, sizes about 40 nm, negative surface charges and high stability. The as-prepared nanoparticles were functionalized with carbosilane cationic dendrons through a carbodiimide-mediated reaction achieving positively charged surfaces. Antisense oligonucleotides were electrostatically attached to nanoparticles surface to perform gene-silencing studies. These complexes were non-haemolytic and non-cytotoxic at the concentrations required. The ability of the complexes to impart cellular uptake was also promising. Therefore, these novel nanoparticulate complexes might be considered as potential non-viral carriers in antisense therapy.

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Section: Nano-emulsion Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel non-viral gene delivery systems composed of carbosilane dendron functionalized nanoparticles prepared from nano-emulsions as non-viral carriers for antisense oligonucleotides

Fornaguera

Grijalvo

Galán

et al. 2015

International Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

show abstract

Formation of Nanoemulsions by Low‐Energy Methods and Their Use as Templates for the Preparation of Polymeric Nanoparticles

Abstract: The sections in this article are Introduction Use of Nano‐emulsions as Templates for the Preparation of Polymeric Nanoparticles

Cited by 1 publication

References 73 publications

Novel non-viral gene delivery systems composed of carbosilane dendron functionalized nanoparticles prepared from nano-emulsions as non-viral carriers for antisense oligonucleotides

Novel non-viral gene delivery systems composed of carbosilane dendron functionalized nanoparticles prepared from nano-emulsions as non-viral carriers for antisense oligonucleotides

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