Formation of microsclerotia ofVerticillium dahliae Kleb. on various plant parts of two potato cultivars

Mol, L.; Schölte, K.

doi:10.1007/bf02357927

Cited by 31 publications

(32 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data corroborates the findings of Bhat and Subbarao (2001) that V. dahliae infection of broccoli was limited to the root tissue. A previous study showed that microsclerotia of V. dahliae formed in the aerial parts of several crops more frequently than in the belowground parts (Mol 1995). The study also showed that 81 % of microsclerotia formed on the aerial portions of spring rape (Brassica napus).…”

Section: Discussionmentioning

confidence: 79%

Crop rotation with broccoli suppresses Verticillium wilt of eggplant

et al. 2014

View full text Add to dashboard Cite

In this study, we assessed whether crop rotation with broccoli suppressed eggplant Verticillium wilt. In 6 trials of crop rotation in 5 fields from 2009 to 2013, broccoli rotation was coupled with postharvest residue incorporation before planting eggplant. Meta-analysis of the disease incidence data from all the trials revealed that the combined relative disease risk was 0.53 (95 % confidence interval 0.34-0.83). Thus, the disease incidence of eggplant in crop rotation with broccoli decreased to 53 % of that of eggplant without broccoli rotation. Browning of vascular tissue from Verticillium dahliae infection was observed in broccoli, but was limited to the roots and did not reach the aerial plant portion where V. dahliae forms microsclerotia. Estimation of V. dahliae population in soil using quantitative nested real-time polymerase chain reaction indicated that V. dahliae DNA copy number in soil samples tended to decrease owing to broccoli cultivation.These results suggest that broccoli root could act as a ''decoy'' that traps V. dahliae in soil. Further studies are necessary to elucidate the mechanisms of disease suppression; this is the first report that broccoli cultivation and subsequent incorporation of residue into the soil suppresses Verticillium wilt of eggplant.

show abstract

Section: Discussionmentioning

confidence: 79%

Crop rotation with broccoli suppresses Verticillium wilt of eggplant

et al. 2014

View full text Add to dashboard Cite

show abstract

“…As explained by Zhou et al (2006), the infection process of V. dahliae can be divided into two phases. Phase I begins with the germination of microsclerotia in response to plant root exudates (Mol and Scholte, 1995). The fungus enters the root through epidermis or wounds, crossing cortical root to gain access to immature xylem elements.…”

Section: Causal Agents Of Verticillium Wilt In Peppermentioning

confidence: 99%

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Goicoechea¹,

Garmendia²,

Sánchez‐Díaz³

et al. 2013

Span. j. agric. res.

View full text Add to dashboard Cite

Verticillium dahliae Kleb. is a vascular pathogen that alters water status and growth of pepper plants and causes drastic reductions in yield. Its control is difficult because it can survive in field soil for several years. The application of arbuscular mycorrhizal fungi (AMF) as bioprotector agents against V. dahliae is an alternative to the use of chemicals which, in addition, is more respectful with the environment. The establishment of the mutualistic association of plant roots and AMF involves a continuous cellular and molecular dialogue between both symbionts that includes the preactivation of plant defense responses that may enhance the resistance or tolerance of mycorrhizal plants to soil-borne pathogens. Some AMF can improve the resistance of Capsicum annuum L. against V. dahliae. This is especially relevant for pepper cultivars (i.e. cv. Piquillo) that exhibit high susceptibility to this pathogen. Compared with non-mycorrhizal plants, mycorrhizal pepper can exhibit more balanced antioxidant metabolism in leaves along the first month after pathogen inoculation, which may contribute to delay both the development of disease symptoms and the decrease of photosynthesis in Verticillium-inoculated plants with the subsequent benefit for yield. In stems, mycorrhizal pepper show earlier and higher deposition of lignin in xylem vessels than nonmycorrhizal plants, even in absence of the pathogen. Moreover, AMF can induce new isoforms of acidic chitinases and superoxide dismutase in roots. Mycorrhizal-specif ic induction of these enzymatic activities together with enhanced peroxidase and phenylalanine ammonia-lyase in roots may also be involved in the bioprotection of Verticillium-induced wilt in pepper by AMF.Additional key words: biological control, Capsicum annuum, mycorrhizal symbiosis, Verticillium dahliae Kleb. ResumenRevisión. Los hongos micorrícico arbusculares (MA) como agentes bioprotectores del pimiento frente a la seca inducida por Verticillium spp.Verticillium dahliae Kleb. altera el estado hídrico y el crecimiento de las plantas de pimiento y reduce la producción del fruto. El empleo de hongos micorrícico arbusculares (MA) como bioprotectores contra V. dahliae es una alternativa al uso de productos químicos más respetuosa para el medio ambiente. La asociación entre las raí-ces de las plantas y los hongos MA conlleva un diálogo celular y molecular entre ambos simbiontes, que incluye la preactivación de respuestas de defensa en la planta, lo cual puede incrementar la resistencia o tolerancia de las plantas micorrizadas hacia los patógenos edáficos. Algunos hongos MA incrementan la resistencia del pimiento (Capsicum annuum L.) contra V. dahliae. Esto es especialmente relevante cuando el cultivar (cv. Piquillo, por ejemplo) es muy susceptible hacia el patógeno. En comparación con las plantas no micorrizadas, las hojas de las plantas de pimiento micorrizadas muestran un metabolismo antioxidante más equilibrado a lo largo del primer mes tras la inoculación del patógeno, lo que puede retrasar el desc...

show abstract

“…After germination of microsclerotia, which is inducible by the root exudates of host plants (Mol and Scholte 1995), the fungal hyphae reach the root surface, penetrate the epidermal cells, transverse the root cortex and enter the immature xylem elements (Beckman 1987). Most of the time of its life cycle V. dahliae is constricted to the vascular system, which is a nutrient-limited environment containing low amounts of sugars, inorganic salts, and amino acids, to which the fungus is well adapted (Wood 1961;Dimond 1970;Dixon and Pegg 1972;Green 1981;Pegg 1981Pegg , 1985Van Alfen 1989).…”

Section: Introductionmentioning

confidence: 99%

Differential interactions of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques

et al. 2007

View full text Add to dashboard Cite

The differential interactions of V. longisporum (VL) and V. dahliae (VD) on the root surface and in the root and shoot vascular system of Brassica napus were studied by confocal laser scanning microscopy (CLSM), using GFP tagging and conventional fluorescence dyes, acid fuchsin and acridin orange. VL and VD transformants expressing sGFP were generated by Agrobacterium-mediated transformation. GFP signals were less homogenous and GFP tagging performed less satisfactory than the conventional fluorescence staining when both were studied with CLSM. Interactions of both pathogens were largely restricted to the root hair zone. At 24 h post-inoculation (hpi), hyphae of VL and VD were found intensely interwoven with the root hairs. Hyphae of VL followed the root hairs towards the root surface. At 36 hpi, VL hyphae started to cover the roots with a hyphal net strictly following the grooves of the junctions of the epidermal cells. VL started to penetrate the root epidermal cells without any conspicuous infection structures. Subsequently, hyphae grew intracellularly and intercellularly through the root cortex towards the central cylinder, without inducing any visible plant responses. Colonisation of the xylem vessels in the shoot with VL was restricted to individual vessels entirely filled with mycelium and conidia, while adjacent vessels remained completely unaffected. This may explain why no wilt symptoms occur in B. napus infected with VL. Elevated amounts of fungal DNA were detectable in the hypocotyls 14 days post-inoculation (dpi) and in the leaves 35 dpi. Root penetration was also observed for VD, however, with no directed root surface growth and mainly an intercellular invasion of the root tissue. In contrast to VL, VD started ample formation of conidia on the roots, and was unable to spread systemically into the shoots. VD did not form microsclerotia in the root tissue as widely observed for VL. This study confirms that VD is non-pathogenic on B. napus and demonstrates that non-host resistance against this fungus materializes in restriction of systemic spread rather than inhibition of penetration.

show abstract

Formation of microsclerotia ofVerticillium dahliae Kleb. on various plant parts of two potato cultivars

Cited by 31 publications

References 13 publications

Crop rotation with broccoli suppresses Verticillium wilt of eggplant

Crop rotation with broccoli suppresses Verticillium wilt of eggplant

Arbuscular mycorrhizal fungi (AMF) as bioprotector agents against wilt induced by Verticillium spp. in pepper: a review

Differential interactions of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques

Contact Info

Product

Resources

About