Formation of magnetoliposomes using self-assembling 1,4-dihydropyridine derivative and maghemite γ-Fe2O3 nanoparticles

Abstract

This work demonstrates studies of ferromagnetic nanoparticle functionalization by a lipid-like compound, cationic pyridine amphiphile derived from 1,4-dihydropyridine, 1,1'-{[3,5-bis(dodecyloxycarbonyl)-4-phenyl-1,4-dihydropyridine-2,6-diyl]dimethanediyl}dipyridi-nium dibromide, and determination of optimal conditions for the production of magnetoliposomes. The following methodologies, such as sonication, spontaneous swelling, and reverse-phase evaporation, were used for the preparation of liposomes. Ferrofluid containing γ-Fe 2 O 3 nanoparticles with a positively charged surface was used for the preparation of magnetoliposomes. The obtained soft matter objects were studied by optical microscopy, transmission electron microscopy, and dynamic light scattering techniques. The optimal conditions for the production of magnetoliposomes were found by spontaneous swelling and reverse-phase evaporation methods using ferrofluid with positively charged γ-Fe 2 O 3 nanoparticles and cationic 1,4-dihydropyridine derivative.

Evaluation of Physicochemical Properties of Amphiphilic 1,4-Dihydropyridines and Preparation of Magnetoliposomes

“…Preparation of magnetoliposomes in this study is a method to evaluate the influence of the 1,4-DHP structure on ML formation. The reversephase evaporation (REV) method along with the use of 1,4-DHP amphiphiles has proved its applicability to produce MLs [41][42][43]. The obtained results may add knowledge for the further comprehension of the structure-activity and liposome parameter relationships of these tested compounds.…”

Liposome-polymer complex for drug delivery system and vaccine stabilization

“…Drug release from MNPs has both a time-and concentrationdependent pattern [55,56,57,58,59,60]. Magnetic nanoparticles can be encapsulated into liposomes using the thin-film hydration method [61,62] or reverse-phase evaporation method [63,64,65]. Hydrophobic magnetic nanoparticles will be integrated into the liposome bilayer membrane, while hydrophilic magnetic nanoparticles will be integrated into the liposome core.…”

Multilobed Magnetic Liposomes Enable Remotely Controlled Collection, Transport, and Delivery of Membrane-Soluble Cargos to Vesicles and Cells

“…Recently, it has been proposed to enhance the functional properties of liposomes by forming composites with various types of magnetic nanoparticles, the so-called magnetoliposomes. − These composites are promising, e.g., in the field of theranostics where they could enable simultaneous bioimaging by MRI (magnetic resonance imaging), thermosensitive release, and tissue-specific drug delivery controlled by an external magnetic field. − The attempts to incorporate magnetic nanoparticles into the liposomal structures are mostly based on (i) encapsulation into the aqueous lumen, (ii) direct integration into the lipidic shell, or (iii) adsorption to the external surface of liposomes. − …”