Formation of lysozyme fibrils on a solid support

Ukraintsev, Egor; Kiselev, G. A.; Kudrinskii, A. A.; Лисичкин, Г. В.; Yaminskii, I. V.

doi:10.1134/s1560090407010022

Cited by 8 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell selectivity is thus not determined merely by FBS layer presence. More subtle differences must be considered for explaining the selective adsorption, such as protein denaturation on hydrophobic surfaces [ 33 , 34 ]. Detailed studies of surface morphology revealed clear differences in surface roughness, morphological features and phase images between the protein layers on H- and O-terminated diamond.…”

Section: Discussionmentioning

confidence: 99%

Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

Rezek

Michalíková

Ukraintsev

et al. 2009

Sensors

View full text Add to dashboard Cite

Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm2) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

Rezek

Michalíková

Ukraintsev

et al. 2009

Sensors

View full text Add to dashboard Cite

show abstract

“…It is probably due to intermolecular interaction between protein molecules. For example, interaction between lysozyme may lead to the bending of AFM cantilever 12 and even to formation of fibrils 13. FBS layer cannot bend diamond substrate, so the internal stress may lead to formation of the depletion region on the border.…”

Section: Discussionmentioning

confidence: 99%

Long‐term adsorption of fetal bovine serum on H/O‐terminated diamond studied in situ by atomic force microscopy

Ukraintsev

Rezek

Kromka

et al. 2009

Physica Status Solidi (b)

View full text Add to dashboard Cite

We investigate adhesion of fetal bovine serum (FBS) on diamond surfaces which have alternating H/O-terminated surface patterns of 30 mm width prepared by hydrogen and oxygen plasma. The samples are immersed into 15% FBS in McCoy's 5A supporting medium and characterized by in situ atomic force microscopy (AFM) during 6 days of adsorption. Within the first day, 2-4 nm primary layer is formed on both H-/O-terminated surfaces. After 6 days, we observe 17 AE 5 nm FBS layer on O-terminated surface and 35 AE 5 nm layer on H-terminated surface. Adhesion of the primary and secondary layer is weaker on H-terminated surface. We present a model of FBS protein layers on H-/O-terminated diamond and we discuss implications for a preferential cell growth on O-terminated diamond. We also show that the preferential cell growth is not affected by the long-term FBS pre-adsorption.

show abstract

“…The cell selectivity is thus not determined merely by FBS layer presence. More subtle differences must be considered for explaining the selective adsorption, such as protein denaturation on hydrophobic surfaces (Ukraintsev, 2007;Zeng, 1999). Detailed studies of surface morphology revealed clear differences in surface roughness, morphological features and phase images between the protein layers on H-and O-terminated diamond.…”

Section: Discussionmentioning

confidence: 99%

Selective Osteoblastic Cell Micro-Arrays on Diamond Films

Rezek¹,

Michalíková²,

Ukraintsev³

et al. 2009

Proceedings of the International Conference on Biomedical Electronics and Devices

View full text Add to dashboard Cite

Unique combination of chemical and biocompatible properties with semiconducting properties makes diamond an attractive material for merging solid state and biological systems. Microscopic chemical patterning of diamond films by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly on the diamond is further controlled and optimized by cell and protein (fetal bovine serum-FBS) concentration. The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged into arrays on O-terminated patterns. The best cell selectivity is achieved for the lowest cell concentrations of 2500 cells/cm 2. Higher cell concentrations enable to colonize unfavorable H-terminated regions due to mutual cell communication. Based on AFM, the proteins are present on both Hand O-terminated surfaces, however, pronounced differences in the thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and hence the cell selectivity. There is no cell selectivity when no protein is supplemented in the medium. These results may be applicable in tissue engineering, implants, bio-electronics, and biotechnology in general.

show abstract

Formation of lysozyme fibrils on a solid support

Cited by 8 publications

References 9 publications

Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

Long‐term adsorption of fetal bovine serum on H/O‐terminated diamond studied in situ by atomic force microscopy

Selective Osteoblastic Cell Micro-Arrays on Diamond Films

Contact Info

Product

Resources

About