Formation of hydrogen peroxide by isolated cell walls from horseradish (Armoracia lapathifolia Gilib.)

Abstract

Isolated cell-wall suspensions from horseradish in the presence of 5×10(-4) M MnCl2 catalyze the production of hydrogen peroxide at the expense of either NADPH or NADH. This reaction is inhibited by scavengers of the superoxide free radical ion such as ascorbate or dihydroxyphenols or by superoxide dismutase, and stimulated by monophenols such as p-coumaric acid. On comparison with isolated (commercial) horseradish peroxidase it becomes evident that (a) cell-wall-bound peroxidase(s) is (are) responsible for the production of hydrogenperoxide, involving the superoxide free radical ion as an intermediate of the complex reaction chain.

Role of Peroxidase in Lignification of Tobacco Cells

“…With coniferyl alcohol as cofactor, only one pH optimum at 4.5 was found. These findings are in contrast to those obtained earlier in which H202 formation was determined by the ['4CJglyoxylate method (2,9). However, with this method in which the nonenzymic oxidation of glyoxylate is coupled to the enzymic oxidation of NADH, the measured pH optimum is not that of NADH oxidation alone (9).…”

“…The polymerization of these phenols is catalyzed by peroxidase in the presence of H202 (2,3). The formation of H202 is also catalyzed by peroxidase resulting from an oxidation ofNADH (2,7). We have studied the role ofdifferent isoenzyme groups of tobacco peroxidase in both reactions (6,9).…”

Differential Activity of Peroxidase Isozymes in Response to Wounding, Gypsy Moth, and Plant Hormones in Northern Red Oak (Quercus rubra L.)

“…PODs comprise one important class of PR proteins (PR-9) implicated in these "defense responses," in which an important role is to catalyze the formation of phenolic radicals at the expense of H 2 O 2 (Gaspar et al, 1985). PODs may also oxidize phenolic monomers to form lignin (Siegel, 1954;Mäder et al, 1980;Grisebach, 1981), function in H 2 O 2 production (Elstner and Heupel, 1976;Mäder et al, 1980), and metabolize indole acetic acid (Endo, 1968;Mato et al, 1988). Each plant species typically displays a unique pattern and number of soluble and wall-bound isozymes that may respond differentially to environmental stimuli.…”

The Role of Hydrogen Peroxide-Producing and Hydrogen Peroxide-Consuming Peroxidases in the Leaf Apoplast of Cowpea in Manganese Tolerance

“…In the plant apoplast, they were attributed to act as hydrogen peroxide (H 2 O 2 )-consuming and phenol-oxidizing enzymes, thus leading to secondary cell wall and lignin formation (Polle et al, 1994;Sato et al, 1995;Christensen et al, 1998;Kärkönen et al, 2002). Peroxidases using NADH as electron donor were also termed NADH oxidases and have been suggested to play an important role in the formation of H 2 O 2 needed for lignification (Elstner and Heupel, 1976;Gross et al, 1977;Halliwell, 1978;Mäder et al, 1980;Mäder and Amberg-Fisher, 1982;Bolwell et al, 1995). Mn and phenolic compounds presumably control the activity of the NADH peroxidase (Elstner and Heupel, 1976;Gross et al, 1977;Halliwell, 1978;Mäder and Fü ssl, 1982;Stich and Ebermann, 1984;Pedreñ o et al, 1987).…”

“…Peroxidases using NADH as electron donor were also termed NADH oxidases and have been suggested to play an important role in the formation of H 2 O 2 needed for lignification (Elstner and Heupel, 1976;Gross et al, 1977;Halliwell, 1978;Mäder et al, 1980;Mäder and Amberg-Fisher, 1982;Bolwell et al, 1995). Mn and phenolic compounds presumably control the activity of the NADH peroxidase (Elstner and Heupel, 1976;Gross et al, 1977;Halliwell, 1978;Mäder and Fü ssl, 1982;Stich and Ebermann, 1984;Pedreñ o et al, 1987). Proteins purified from the apoplastic washing fluid (AWF) of leaves from the Mnsensitive cowpea cv TVu 91 showed NADH-peroxidase activity, which was strongly enhanced at high leaf Mn concentrations (Fecht-Christoffers et al, 2003a).…”