Formation of histamine and tyramine by lactic acid bacteria in decarboxylase assay medium

Abstract

A modified decarboxylase assay medium (DCA medium) was used for studying the production of biogenic amines by Leuconostoc oenos DSM 20252 and two strains of Lactobacillus buchneri (Lb14 and St2A). The DCA medium contained histidine, lysine, ornithine and tyrosine as precursors of the respective biogenic amines. Under the experimental conditions both strains of Lact. buchneri produced > 90% of the maximum amount of histamine within 24 h. Only tyramine was produced by Leuc. oenos DSM 20252. accountine for 88% of the maximum theoretical amount within 24 h.

Biogenic amines in red wine: The impact of technological processing of grape and wine / BIOGENI AMINI V RDEČEM VINU: VPLIV TEHNOLOŠKE PREDELAVE GROZDJA IN VINA

“…Number of tyramine-producing LAB in wine that had undergone MLF were identified and isolated by Moreno-Arribas et al (2000) and all of them belong to Lactobacilli. As the literature suggest, no tyramine-producing O. oeni strain has yet been reported except of one strain (O. oeni DSM 2025) that was shown to be able to produce tyramine in a defined growth medium (Choudhury et al, 1990). This is confirmed by Sebastian et al (2011) who observed formation of BA for all 57 strains of Lactobacillus brevis.…”

Factors influencing biogenic amine production by a strain of Oenococcus oeni in a model system

“…In strains belonging to this species isolated from wine, tyrosine decarboxylation was not detected by Delfini (1989), Lonvaud-Funel andJoyeux (1994), Guerrini, Mangani, Granchi, and Vincenzini (2002) and Moreno-Arribas, Polo, Jorganes, and Mun˜oz (2002). Nevertheless, tyramine is the BA more commonly produced by LAB (Bover-Cid & Holzapfel, 1999;Silla-Santos, 1996) and O. oeni DSM 20252 was able to produce tyramine in fermented carrots and synthetic medium (Choudhury, Hansen, Engesser, Hammes, & Holzhapfel, 1990).…”

Biogenic Amines in Wine: Understanding the Headache

“…O. oeni seems to have a low distribution of the metabolic ability to produce tyramine (Moreno-Arribas et al, 2000;Geurrini et al, 2002). As far as literature suggested in 2003, no tyramine producing O. oeni strain had yet been reported (Moreno-Arribas et al, 2003), with the exception of a strain (O. oeni DSM 2025) that was shown to be able to produce tyramine in a laboratory medium (Choudhury et al, 1990). Ornithine decarboxylase Marcobal et al (2004) reported that a putrescine producing strain of O. oeni (IOEB 8419) was isolated from ropy red wine by Coton et al (1999) and was suspected of having ornithine decarboxylase (ODC) activity.…”

“…Modifications of the screening plate medium have been made by various researchers to make it more suitable for the growth of lactic acid bacteria and activity of decarboxylase enzymes. Improvements have also led to greater sensitivity and reliability of the screening plate method, and it has shown good correlation with other chemical analytical methods (Choudhury, 1990;Maijala, 1993;Bover-Cid & Holzapfel, 1999). Yet, it is still advisable to confirm the results of simple screening methods by another analytical method (such as HPLC), because false positive results can occur due to the formation of other alkaline compounds (such as ammonia), and failure to detect amine production has also been reported (Moreno-Arribas et al, 2003).…”