Formation of Giant Vesicles from Different Kinds of Lipids Using the Electroformation Method

Fischer, Aline; Luisi, Pier Luigi; Oberholzer, Thomas; Walde, Peter

doi:10.1002/9780470511534.ch4

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…Permeability of the POPC membrane for glycerol, calculated from(12) for five recorded transfers of the POPC vesicle into an iso-osmolar solution of glycerol.…”

mentioning

confidence: 99%

“…While the electroformation method is appreciated for its superior homogeneity of vesicle sizes [11] and is widely recognized as a simple and reproducible technique which appears to be efficient for many types of different lipid mixtures [12], its key disadvantage is that it cannot be applied if the salt concentration exceeds 10 mM [13], or when the membrane is composed of charged amphiphiles [14]. Methods based on spontaneous swelling [15,16] were used for the preparation of GUVs in physiological conditions [17,18,19].…”

Section: Introductionmentioning

confidence: 99%

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Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Peterlin

Arrigler

2008

Colloids and Surfaces B: Biointerfaces

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A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152-160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an iso-osmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P = (2.09±0.82)·10−8 m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.

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“…Permeability of the POPC membrane for glycerol, calculated from(12) for five recorded transfers of the POPC vesicle into an iso-osmolar solution of glycerol.…”

mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Peterlin

Arrigler

2008

Colloids and Surfaces B: Biointerfaces

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“…Several preparation methods of giant liposomes have been developed [9]. The gentle hydration method is one of the conventional methods used to produce liposomes [10,11], and this method involves the spontaneous swelling of dried lipid films in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

Electroformation of giant liposomes in microfluidic channels

Kuribayashi¹,

Tresset²,

Coquet³

et al. 2006

Meas. Sci. Technol.

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We present an on-chip method to produce various types of giant liposomes using electroformation in microfluidic channels. These channels were sandwiched between glass slides coated with indium tin oxide (ITO) electrodes. Giant liposomes were formed inside the channels by applying an ac voltage. Important characteristics of the obtained liposomes were investigated quantitatively. We found that 54% of the liposomes produced by electroformation had diameters exceeding 10 µm and that 90% of the liposomes did not enclose extra liposomes inside themselves. Using two microfluidic channels, we found that giant liposomes with nano/micrometre- sized materials encapsulated were formed simultaneously on a chip.

show abstract

Formation of Giant Vesicles from Different Kinds of Lipids Using the Electroformation Method

Cited by 2 publications

References 23 publications

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Electroformation of giant liposomes in microfluidic channels

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