2011
DOI: 10.1002/cbic.201100537
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Formation of Giant Protein Vesicles by a Lipid Cosolvent Method

Abstract: This paper describes a method to create giant protein vesicles (GPVs) of ≥10 μm by solvent-driven fusion of large vesicles (0.1-0.2 μm) with reconstituted membrane proteins. We found that formation of GPVs proceeded from rotational mixing of protein-reconstituted large unilamellar vesicles (LUVs) with a lipid-containing solvent phase. We made GPVs by using n-decane and squalene as solvents, and applied generalized polarization (GP) imaging to monitor the polarity around the protein transmembrane region of aqua… Show more

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Cited by 12 publications
(12 citation statements)
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“…Moreover, it would be fascinating to see whether colonies can divide in branches, and perhaps grow again, or revert to isolated cells. The construction of colonies from GVs containing membrane proteins, [33] perhaps as a model of primitive hydrophobic polypeptides, would also be interesting.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, it would be fascinating to see whether colonies can divide in branches, and perhaps grow again, or revert to isolated cells. The construction of colonies from GVs containing membrane proteins, [33] perhaps as a model of primitive hydrophobic polypeptides, would also be interesting.…”
Section: Discussionmentioning
confidence: 99%
“…Both membrane proteins were homogeneously incorporated in the membranes and biologically active as demonstrated by Ca 2+ or H + pumping. Functionally active aquaporins have also been embedded into GUV membranes either by mixing protein-reconstituted liposomes of 0.1 to 0.2 μm diameter with a lipid-containing oil phase (lipid cosolvent method) [49] or by swelling of a tissue-like giant vesicle film [50]. For the first time, Dezi et al recently incorporated different transmembrane proteins like bacteriorhodopsin and the ferrichrome transport protein FhuA into GUVs with lipid mixtures representative of cellular plasma membranes [51].…”
Section: Reviewmentioning
confidence: 99%
“…Our current operation region is shown in the white box. To further enhance the performance, additional research will be needed to prepare AqpZembedded giant unilamellar vesicles [23] or choose better blends of ABA block copolymers so as to increase the AqpZ incorporation ratio and ultimately increase the vesicle permeability.…”
Section: Introductionmentioning
confidence: 99%