Formation of Giant Protein Vesicles by a Lipid Cosolvent Method

Hansen, Jesper S.; Vararattanavech, Ardcharaporn; Vissing, Thomas; Torres, Jaume; Emnéus, Jenny; Hélix-Nielsen, Claus

doi:10.1002/cbic.201100537

Cited by 12 publications

(12 citation statements)

References 34 publications

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“…Moreover, it would be fascinating to see whether colonies can divide in branches, and perhaps grow again, or revert to isolated cells. The construction of colonies from GVs containing membrane proteins, [33] perhaps as a model of primitive hydrophobic polypeptides, would also be interesting.…”

Section: Discussionmentioning

confidence: 99%

Giant Vesicles “Colonies”: A Model for Primitive Cell Communities

2012

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Current research on the origin of life typically focuses on the self-organisation of molecular components in individual cell-like compartments, thereby bringing about the emergence of self-sustaining minimal cells. This is justified by the fact that single cells are the minimal forms of life. No attempts have been made to investigate the cooperative mechanisms that could derive from the assembly of individual compartments. Here we present a novel experimental approach based on vesicles "colonies" as a model of primitive cell communities. Experiments show that several advantages could have favoured primitive cell colonies when compared with isolated primitive cells. In fact there are two novel unexpected features typical of vesicle colonies, namely solute capture and vesicle fusion, which can be seen as the basic physicochemical mechanisms at the origin of life.

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Section: Discussionmentioning

confidence: 99%

Giant Vesicles “Colonies”: A Model for Primitive Cell Communities

2012

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“…Both membrane proteins were homogeneously incorporated in the membranes and biologically active as demonstrated by Ca 2+ or H + pumping. Functionally active aquaporins have also been embedded into GUV membranes either by mixing protein-reconstituted liposomes of 0.1 to 0.2 μm diameter with a lipid-containing oil phase (lipid cosolvent method) [49] or by swelling of a tissue-like giant vesicle film [50]. For the first time, Dezi et al recently incorporated different transmembrane proteins like bacteriorhodopsin and the ferrichrome transport protein FhuA into GUVs with lipid mixtures representative of cellular plasma membranes [51].…”

Section: Reviewmentioning

confidence: 99%

Model systems for studying cell adhesion and biomimetic actin networks

Brüggemann¹,

Frohnmayer²,

Spatz³

2014

Beilstein J. Nanotechnol.

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SummaryMany cellular processes, such as migration, proliferation, wound healing and tumor progression are based on cell adhesion. Amongst different cell adhesion molecules, the integrin receptors play a very significant role. Over the past decades the function and signalling of various such integrins have been studied by incorporating the proteins into lipid membranes. These proteolipid structures lay the foundation for the development of artificial cells, which are able to adhere to substrates. To build biomimetic models for studying cell shape and spreading, actin networks can be incorporated into lipid vesicles, too. We here review the mechanisms of integrin-mediated cell adhesion and recent advances in the field of minimal cells towards synthetic adhesion. We focus on reconstituting integrins into lipid structures for mimicking cell adhesion and on the incorporation of actin networks and talin into model cells.

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“…Our current operation region is shown in the white box. To further enhance the performance, additional research will be needed to prepare AqpZembedded giant unilamellar vesicles [23] or choose better blends of ABA block copolymers so as to increase the AqpZ incorporation ratio and ultimately increase the vesicle permeability.…”

Section: Introductionmentioning

confidence: 99%