Formation of Fruiting Body of Omphalotus japonicus by Sawdust Cultivation

Abstract

Omphalotus japonicus is known to as a poisonous mushroom in East Asia, but Illudin S, bioluminescent compound, has been recently reported for an anticancer effect. The fungus is quite rare in natural condition in Korea. Therefore, we tried to cultivate in the mushroom on the sawdust cultivation to provide sufficient amount of mushroom for pharmaceutical study for the future. The mushroom could be harvested from all sawdust media used in this study, and the sawdust of Pinus densiflora was considered to be the best for mushroom cultivation with considering mushroom productivity and cultivation period. The mushroom was produced 43~80 g fresh weight per 600 g sawdust medium in average, and could be harvested one to three times during cultivation period.

Cloning and Expression Analysis of Bioluminescence Genes in Omphalotus guepiniiformis Reveal Stress-Dependent Regulation of Bioluminescence

“…Taking together our data and previous reports on various responses of fungal bioluminescence to different environmental conditions [ 15–22 ], luminous fungi seem to actively regulate bioluminescence via diverse molecular mechanisms to cope with environmental stresses. Because not only diverse factors affecting bioluminescence intensity but also high-quality genome information and the conditions for artificial cultivation of fruiting body of O. guepiniiformis have been described [ 12 , 41 ], it is possible to investigate the effects of various factors on bioluminescence at the molecular level throughout the whole life cycle, from vegetative mycelium to fruiting body and spores. Therefore, O. guepiniiformis can be a model species for studying fungal bioluminescence and fungal signal transduction under various conditions.…”