Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus

Watanabe, Rihito; Kakizaki, Masatoshi; Ikehara, Yuzuru; Togayachi, Akira

doi:10.1111/neup.12302

Cited by 14 publications

(38 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mice were maintained according to guidelines set by Soka University. At scheduled time periods after inoculation, organs including the brains and spleens were removed from mice after exsanguination under deep anesthesia, and were embedded in Tissue Tek OCT (Sakura, Tokyo, Japan) and snap frozen to prepare frozen sections …”

Section: Methodsmentioning

confidence: 99%

“…The ECM includes an antigen recognized by Erasmus University Rotterdam‐thymic reticulum antibody 7 (ER‐TR7) . ER‐TR7‐antigen (ERag)‐positive fibers (ERfibs) associated with laminin and collagen III function as a conduit system for viral entry into the brain parenchyma from the meninges . Studying the initial phase of infection in our previous report, the formation of ECMs other than ERag were detected in the ventricular and paraventricular zone, and around the blood vessels, but it was not prominent in the brain parenchyma in vivo .…”

Section: Introductionmentioning

confidence: 93%

“…ER‐TR7‐antigen (ERag)‐positive fibers (ERfibs) associated with laminin and collagen III function as a conduit system for viral entry into the brain parenchyma from the meninges . Studying the initial phase of infection in our previous report, the formation of ECMs other than ERag were detected in the ventricular and paraventricular zone, and around the blood vessels, but it was not prominent in the brain parenchyma in vivo . Therefore, in this report, we studied ECM formation in the brain during the later phase of infection at 72 hpi.…”

Section: Introductionmentioning

confidence: 97%

“…At 48 hpi, the passage was found to be constructed by the extracellular matrix (ECM) between the ventricle and meninges at the cerebellopontine angle . The ECM includes an antigen recognized by Erasmus University Rotterdam‐thymic reticulum antibody 7 (ER‐TR7) .…”

Section: Introductionmentioning

confidence: 99%

“…The main construct of FRN is maintained by ERag‐producing fibroblastic reticular cells (FRCs) in the lymphiod organs . In the CNS, ERag is produced by almost all cell types that constitute the CNS, and the most prominent ERag‐producing cells in the brain parenchyma are astrocytes . However, it has yet to be elucidated what cell type in the brain parenchyma produces the components of ERfibs other than ERag.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Extracellular matrix in the CNS induced by neuropathogenic viral infection

Watanabe

Kakizaki

2017

Neuropathology

Self Cite

View full text Add to dashboard Cite

During the early phase of infection with an extremely neurovirulent murine coronavirus, cl-2, the ER-TR7 antigen (ERag)-positive fibers (ERfibs) associated with laminin and collagen III show a rapid increase in expression levels in the meninges, followed by an appearance of the antigens in the ventricle and brain parenchyma. Then, cl-2 invades the ventricle and ventricular wall along the newly assembled ERfibs after infection, using them as a pathway from the meninges, the initial site of infection. In the lymph nodes and spleen, ERag is mainly produced by fibroblastic reticular cells (FRCs), which play a key role in nursing the ERfibs to form a fibroblastic reticular network (FRN). The FRN functions as a conduit system to transfer antigens, cytokines or leukocytes in the lymphoid organs. In the brain parenchyma, astrocytes were found to produce the main components of mature ERfibs, such as collagen, laminin and ERag, which have been identified in the lymphoid organs. The producibility of these extracellular matrices (ECMs) by astrocytes was further confirmed by primary brain cultures, which disclosed the dissociation of laminin and ERag production, and the close association of ERag production with that of collagen, forming a fibrous structure. The pattern of ECM production in vitro indicated the process of forming mature ERfibs in the brain, that is, fibers made of collagen fibers and ERag are wrapped by laminin prepared as a sheet structure. In addition, the brain parenchymal cells that produce interferon β after infection in spite of their residence away from the sites of viral invasion were surrounded by ERfibs, which were closely associated with astrocytic fibers. These findings indicate that astrocytes play a central role in forming the astrocytic reticular network (ARN) in the brain parenchyma, as FRCs do to form FRN in the lymphoid organs.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Extracellular matrix in the CNS induced by neuropathogenic viral infection

Watanabe

Kakizaki

2017

Neuropathology

Self Cite

View full text Add to dashboard Cite

show abstract

New advances in CNS immunity against viral infection

Manglani

McGavern

2018

Current Opinion in Virology

View full text Add to dashboard Cite

The central nervous system (CNS) is an immunologically specialized organ where restrictive barrier structures protect the parenchyma from inflammation and infection. This protection is important in preventing damage to non-renewable resident cell populations, such as neurons, responsible for functions ranging from executive to autonomic. Despite these barriers, the CNS can be infected through several entry portals, giving rise to meningitis and encephalitis. Following infection, resident cells recruit peripherally derived immune cells to sites of viral infection. In this review, we discuss recent advances in immune recruitment and entry at barrier structures as well as current immunotherapeutic strategies for the treatment of persistent viral infections.

show abstract

Advances in Meningeal Immunity

Rua

McGavern

2018

Trends in Molecular Medicine

201

241

View full text Add to dashboard Cite

The central nervous system (CNS) is an immunologically specialized tissue protected by a blood-brain barrier. The CNS parenchyma is enveloped by a series of overlapping membranes that are collectively referred to as the meninges. The meninges provide an additional CNS barrier, harbor a diverse array of resident immune cells, and serve as a crucial interface with the periphery. Recent studies have significantly advanced our understanding of meningeal immunity, demonstrating how a complex immune landscape influences CNS functions under steady-state and inflammatory conditions. The location and activation state of meningeal immune cells can profoundly influence CNS homeostasis and contribute to neurological disorders, but these cells are also well equipped to protect the CNS from pathogens. In this review, we discuss advances in our understanding of the meningeal immune repertoire and provide insights into how this CNS barrier operates immunologically under conditions ranging from neurocognition to inflammatory diseases.

show abstract

Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus

Cited by 14 publications

References 63 publications

Extracellular matrix in the CNS induced by neuropathogenic viral infection

Extracellular matrix in the CNS induced by neuropathogenic viral infection

New advances in CNS immunity against viral infection

Advances in Meningeal Immunity

Contact Info

Product

Resources

About