“…The present study was intended to delineate the extent to which this enzyme series could contribute to the overall rate of generation of free radicals within the liver under basal conditions. The generation of reactive oxygen species was assayed by the use of a fluorescent probe, the utility of which has been documented previously [6]. The level of ROS production was determined in the presence and absence of cytochrome P450 substrates, and the effect of an inhibitor of mixed-function oxidase was also studied.…”